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The map label for this gene is bcsZ [H]

Identifier: 157368399

GI number: 157368399

Start: 163917

End: 165029

Strand: Direct

Name: bcsZ [H]

Synonym: Spro_0150

Alternate gene names: 157368399

Gene position: 163917-165029 (Clockwise)

Preceding gene: 157368398

Following gene: 157368400

Centisome position: 3.01

GC content: 58.76

Gene sequence:

>1113_bases
ATGCCTGCGGCATTGAAGCGTCTGACCTTCGGCATGTTGCTACTGTGCTCATTCAGCGCGGTAGCCACCTGCGAGTGGCC
AGCCTGGCAACAGTACAAACAGTTTTATATCAGCGAGCAGGGGCGGGTGATCGATCCGAGTAGCCCGAATCGAATCACGA
CCTCGGAAGGCCAGAGTTATGGCCTGTTCTTCGCGCTGGTGGCCAACGATCGACCTGCTTTCGACCAACTGCTGACGTGG
ACGGAAAATAATCTGGCGGCGGGCGATCTCAGCGCCCATTTGCCGGCCTGGCTATGGGGTGAGGACGATAAAAAGCAGTG
GACGGTGCTGGACAGTAATTCGGCCTCCGATTCCGATCTGTGGATTGCCTATAACCTGCTGGAGGCGGGGCGCCTGTGGA
AAAGCCGTCGCTATCAAACTCTCGGTACCCTGCTGCTGCAGCGGATTGCCCGTGAAGAAGTGGCTGATATTCCCGGCCTG
GGGCTGATGTTGTTACCGGGTAAGGTGGGGTTTGTGGCGGAAGATCGCTGGCGGCTCAACCCAAGCTATCTGCCGCCGCA
ACTGTTGGCGCGTTTTGCCGCGTTGAACGGCCCATGGCGTGCGATGCAGCAAACTAATCAGCGGCTATGGCTGGAGACGG
CCCCTTACGGCTTCTTGCCGGACTGGGTGGTGTGGCAGGTGGGCAAAGGCTGGCAACCGGACACCGTCAAGCCGAACATC
GGCAGTTACGACGCCATCCGGGCCTATTTGTGGGCCGGTATGCTGGCAGACGACGATCAACACAAGGCGGCGCTGCTCAA
GCAGTTGCAACCTATGGCACAGCTCACCGTTCAACAGGGCGTGCCGCCGGAGAAAACCGACACCGCCAGCGGCAAAACCA
TGGGTAACGGCCCGGTAGGTTTTTCTGCCTCGATGTTGCCGATGCTGGCCAATCAACCCGAGGCGTTACAGGTACAACGC
CAACGCATTAAGCAAAACCCGCCGGGCGACAACGCCTATTTCAGCGCCTCGCTGGCGCTGTTTGGCCAGGGATGGGATCA
ACAACGTTATCGTTTTAATCGGCAGGGTGAACTTCAACCCTCCTGGGGCGGCCAATGCGTAACTTCCGAATAA

Upstream 100 bases:

>100_bases
TTGCGGTCGTGGTGGTGGTGATCCTGGCGCTGTTGTTGTGGCGTGGTTTGAAAGCCTTCAGCCGTCGTCGCCTGTCGCCT
GAAGATCGGGACTAATCGCT

Downstream 100 bases:

>100_bases
ACTGGCTGGGTCTGGTGCCTTTAAGTCTGGCGATGTTGCCACAGGCGCAGGGGGCAGAAACCGTCGCGCCGGAACAGTGG
CTGCTGGAGCAGGTACGGGT

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPAALKRLTFGMLLLCSFSAVATCEWPAWQQYKQFYISEQGRVIDPSSPNRITTSEGQSYGLFFALVANDRPAFDQLLTW
TENNLAAGDLSAHLPAWLWGEDDKKQWTVLDSNSASDSDLWIAYNLLEAGRLWKSRRYQTLGTLLLQRIAREEVADIPGL
GLMLLPGKVGFVAEDRWRLNPSYLPPQLLARFAALNGPWRAMQQTNQRLWLETAPYGFLPDWVVWQVGKGWQPDTVKPNI
GSYDAIRAYLWAGMLADDDQHKAALLKQLQPMAQLTVQQGVPPEKTDTASGKTMGNGPVGFSASMLPMLANQPEALQVQR
QRIKQNPPGDNAYFSASLALFGQGWDQQRYRFNRQGELQPSWGGQCVTSE

Sequences:

>Translated_370_residues
MPAALKRLTFGMLLLCSFSAVATCEWPAWQQYKQFYISEQGRVIDPSSPNRITTSEGQSYGLFFALVANDRPAFDQLLTW
TENNLAAGDLSAHLPAWLWGEDDKKQWTVLDSNSASDSDLWIAYNLLEAGRLWKSRRYQTLGTLLLQRIAREEVADIPGL
GLMLLPGKVGFVAEDRWRLNPSYLPPQLLARFAALNGPWRAMQQTNQRLWLETAPYGFLPDWVVWQVGKGWQPDTVKPNI
GSYDAIRAYLWAGMLADDDQHKAALLKQLQPMAQLTVQQGVPPEKTDTASGKTMGNGPVGFSASMLPMLANQPEALQVQR
QRIKQNPPGDNAYFSASLALFGQGWDQQRYRFNRQGELQPSWGGQCVTSE
>Mature_369_residues
PAALKRLTFGMLLLCSFSAVATCEWPAWQQYKQFYISEQGRVIDPSSPNRITTSEGQSYGLFFALVANDRPAFDQLLTWT
ENNLAAGDLSAHLPAWLWGEDDKKQWTVLDSNSASDSDLWIAYNLLEAGRLWKSRRYQTLGTLLLQRIAREEVADIPGLG
LMLLPGKVGFVAEDRWRLNPSYLPPQLLARFAALNGPWRAMQQTNQRLWLETAPYGFLPDWVVWQVGKGWQPDTVKPNIG
SYDAIRAYLWAGMLADDDQHKAALLKQLQPMAQLTVQQGVPPEKTDTASGKTMGNGPVGFSASMLPMLANQPEALQVQRQ
RIKQNPPGDNAYFSASLALFGQGWDQQRYRFNRQGELQPSWGGQCVTSE

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=359, Percent_Identity=59.6100278551532, Blast_Score=453, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41525; Mature: 41393

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAALKRLTFGMLLLCSFSAVATCEWPAWQQYKQFYISEQGRVIDPSSPNRITTSEGQSY
CCHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCEECCCCCCEEECCCCCCC
GLFFALVANDRPAFDQLLTWTENNLAAGDLSAHLPAWLWGEDDKKQWTVLDSNSASDSDL
EEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCE
WIAYNLLEAGRLWKSRRYQTLGTLLLQRIAREEVADIPGLGLMLLPGKVGFVAEDRWRLN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEECC
PSYLPPQLLARFAALNGPWRAMQQTNQRLWLETAPYGFLPDWVVWQVGKGWQPDTVKPNI
CCCCCHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCHHHEECCCCCCCCCCCCCC
GSYDAIRAYLWAGMLADDDQHKAALLKQLQPMAQLTVQQGVPPEKTDTASGKTMGNGPVG
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
FSASMLPMLANQPEALQVQRQRIKQNPPGDNAYFSASLALFGQGWDQQRYRFNRQGELQP
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCC
SWGGQCVTSE
CCCCEEECCC
>Mature Secondary Structure 
PAALKRLTFGMLLLCSFSAVATCEWPAWQQYKQFYISEQGRVIDPSSPNRITTSEGQSY
CHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCEECCCCCCEEECCCCCCC
GLFFALVANDRPAFDQLLTWTENNLAAGDLSAHLPAWLWGEDDKKQWTVLDSNSASDSDL
EEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCCCEEEEEECCCCCCCCE
WIAYNLLEAGRLWKSRRYQTLGTLLLQRIAREEVADIPGLGLMLLPGKVGFVAEDRWRLN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEECC
PSYLPPQLLARFAALNGPWRAMQQTNQRLWLETAPYGFLPDWVVWQVGKGWQPDTVKPNI
CCCCCHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCHHHEECCCCCCCCCCCCCC
GSYDAIRAYLWAGMLADDDQHKAALLKQLQPMAQLTVQQGVPPEKTDTASGKTMGNGPVG
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
FSASMLPMLANQPEALQVQRQRIKQNPPGDNAYFSASLALFGQGWDQQRYRFNRQGELQP
CCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCC
SWGGQCVTSE
CCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]