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The map label for this gene is yiaO [H]

Identifier: 157368372

GI number: 157368372

Start: 131907

End: 132920

Strand: Reverse

Name: yiaO [H]

Synonym: Spro_0123

Alternate gene names: 157368372

Gene position: 132920-131907 (Counterclockwise)

Preceding gene: 157368374

Following gene: 157368371

Centisome position: 2.44

GC content: 56.11

Gene sequence:

>1014_bases
ATGAAATCACTGACATGCCGTTTAACGCCGCGTCTGTTTATCACGCTGTTATTTATGCTGTTTATCTTGCCACTGCGCGC
CAATGCCGCCGAAGTCAGCCTGAGGGTATATTCATCGCTACCCGACGATGGTTACTCCGCGCACGCCATCTGGTTTCACC
GTTTCAAACAAAATCTGGACCAGCGCCTGCCGGGCAAAATCGCCCTTAATTACTTCCCTAACGGCATGCTTGGTAAAGAG
GCCGATGCGGTCCAACAGGTACGTATCGGCGCGATCGACATGACTATCTCCGGGACTTCCATCTGGGGCACGCTGGTGCC
GGAAATCGGCGTGCTGGATCTGGGCTATCTGTTTGACGATAACCAGCAACTGGGTCAGGCGCTGGATGGCCAGGCGGGCC
AACTGCTGAGCGCCCTGCTGGCCAAACAAAGCAATATCAAGGTGCTGGGCTATGGTTTCAGCCTGGGCGCACGCAACATT
TACAGTAAAAAACCGCTGCAGTCACCGCAGGACCTCAACCGGATGAAAGTACGCGTACTGCCGGCCCCCAACTTTATCGC
CACGCTAAAAAGCATGGGTGCCACGGCGATCCCAATGCCCGGCGGTGAGGTTTACTCCGCACTGCAAATGGGAGTGATCG
ACGGTGTGGAACATGACGCCGCCACGGTCTACACCAGCAAGTATTACGAGATTGCCAAATACGCCACGCTGACCCGCCAT
ATCTACAACCCGATTGTCGCCACCATCAGCCAGGCGGCGTTCCAACGCATCCCGCAACCGCTGCAAGCCGACTTCTTGGC
GGCAGCCAGCGAAGCAACGGTTTATGAACGCAAAATCGCAGCGGAAAAAGAAATTCAGGCGATGGACAAACTGAAAGCCG
CCGGTGTGATCTTTACCGAAACCGACCGCGACTATTTCCGCCGGCAAACGCTGCCGCTACAGGCGGCATTTAGCGGGAAA
TACCCACAGGCCAAAGCGGTGCTGGATGCCATTCATGCCACAGCGCAGCCCTAA

Upstream 100 bases:

>100_bases
AAGTATCGTACCCGTTCCCCCTGTCCCTACAGCGTTACCCACAGGGGAAATCATCTTCACCACCAGCATCGCAGGCTGTC
TGCGATCGGGAGCCAGGCTG

Downstream 100 bases:

>100_bases
CGCACGCCTGGCGGAGGATTTGCTATGACCCTTCCTGTAACCCTTGAAATGAGCGTAAAAAAAGTGGCCATCCTGACGCA
GTTAAGCCGCGGTCTGACCC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKSLTCRLTPRLFITLLFMLFILPLRANAAEVSLRVYSSLPDDGYSAHAIWFHRFKQNLDQRLPGKIALNYFPNGMLGKE
ADAVQQVRIGAIDMTISGTSIWGTLVPEIGVLDLGYLFDDNQQLGQALDGQAGQLLSALLAKQSNIKVLGYGFSLGARNI
YSKKPLQSPQDLNRMKVRVLPAPNFIATLKSMGATAIPMPGGEVYSALQMGVIDGVEHDAATVYTSKYYEIAKYATLTRH
IYNPIVATISQAAFQRIPQPLQADFLAAASEATVYERKIAAEKEIQAMDKLKAAGVIFTETDRDYFRRQTLPLQAAFSGK
YPQAKAVLDAIHATAQP

Sequences:

>Translated_337_residues
MKSLTCRLTPRLFITLLFMLFILPLRANAAEVSLRVYSSLPDDGYSAHAIWFHRFKQNLDQRLPGKIALNYFPNGMLGKE
ADAVQQVRIGAIDMTISGTSIWGTLVPEIGVLDLGYLFDDNQQLGQALDGQAGQLLSALLAKQSNIKVLGYGFSLGARNI
YSKKPLQSPQDLNRMKVRVLPAPNFIATLKSMGATAIPMPGGEVYSALQMGVIDGVEHDAATVYTSKYYEIAKYATLTRH
IYNPIVATISQAAFQRIPQPLQADFLAAASEATVYERKIAAEKEIQAMDKLKAAGVIFTETDRDYFRRQTLPLQAAFSGK
YPQAKAVLDAIHATAQP
>Mature_337_residues
MKSLTCRLTPRLFITLLFMLFILPLRANAAEVSLRVYSSLPDDGYSAHAIWFHRFKQNLDQRLPGKIALNYFPNGMLGKE
ADAVQQVRIGAIDMTISGTSIWGTLVPEIGVLDLGYLFDDNQQLGQALDGQAGQLLSALLAKQSNIKVLGYGFSLGARNI
YSKKPLQSPQDLNRMKVRVLPAPNFIATLKSMGATAIPMPGGEVYSALQMGVIDGVEHDAATVYTSKYYEIAKYATLTRH
IYNPIVATISQAAFQRIPQPLQADFLAAASEATVYERKIAAEKEIQAMDKLKAAGVIFTETDRDYFRRQTLPLQAAFSGK
YPQAKAVLDAIHATAQP

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=255, Percent_Identity=36.078431372549, Blast_Score=168, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37046; Mature: 37046

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTCRLTPRLFITLLFMLFILPLRANAAEVSLRVYSSLPDDGYSAHAIWFHRFKQNLD
CCCCEEEECHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHH
QRLPGKIALNYFPNGMLGKEADAVQQVRIGAIDMTISGTSIWGTLVPEIGVLDLGYLFDD
HHCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEEEEECCHHHHHHCCCCCCEEEHEEECC
NQQLGQALDGQAGQLLSALLAKQSNIKVLGYGFSLGARNIYSKKPLQSPQDLNRMKVRVL
CHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
PAPNFIATLKSMGATAIPMPGGEVYSALQMGVIDGVEHDAATVYTSKYYEIAKYATLTRH
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHHHHH
IYNPIVATISQAAFQRIPQPLQADFLAAASEATVYERKIAAEKEIQAMDKLKAAGVIFTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TDRDYFRRQTLPLQAAFSGKYPQAKAVLDAIHATAQP
CCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSLTCRLTPRLFITLLFMLFILPLRANAAEVSLRVYSSLPDDGYSAHAIWFHRFKQNLD
CCCCEEEECHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHH
QRLPGKIALNYFPNGMLGKEADAVQQVRIGAIDMTISGTSIWGTLVPEIGVLDLGYLFDD
HHCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEEEEECCHHHHHHCCCCCCEEEHEEECC
NQQLGQALDGQAGQLLSALLAKQSNIKVLGYGFSLGARNIYSKKPLQSPQDLNRMKVRVL
CHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEE
PAPNFIATLKSMGATAIPMPGGEVYSALQMGVIDGVEHDAATVYTSKYYEIAKYATLTRH
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHHHHH
IYNPIVATISQAAFQRIPQPLQADFLAAASEATVYERKIAAEKEIQAMDKLKAAGVIFTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
TDRDYFRRQTLPLQAAFSGKYPQAKAVLDAIHATAQP
CCHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]