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The map label for this gene is ibpB

Identifier: 157368298

GI number: 157368298

Start: 49568

End: 49996

Strand: Direct

Name: ibpB

Synonym: Spro_0049

Alternate gene names: 157368298

Gene position: 49568-49996 (Clockwise)

Preceding gene: 157368297

Following gene: 157368299

Centisome position: 0.91

GC content: 55.24

Gene sequence:

>429_bases
ATGCGCAATTACGACTTATCCCCACTACTGCGTCAGTGGATCGGCTTTGACAAATTGGCCAGTTCAATGGGCGGCCAGGA
GCCGCAAGGGTTCCCGCCATACAACATCGAGAAAAGCGACGATAACCACTACCGCATTTCTCTGGCGCTGGCCGGCTTCA
AACAAAGCGAACTGGATATTGAAGTCGAAGGCCCACGTCTGACCGTGCGCGGTAAGCCGACACCGTCGGAAAAACAGGTG
GAATATCTGCATCAGGGGTTGGTGTGCAAAGAGTTCGCACTGACCTTCACGCTGGCTGAGCATTTGCAGGTTTCCGAGGC
CCAGTTTGAAAATGGCCTGCTGCATATCGATCTGGTGCGTCAGGTACCGGAAGCCCTGCAGCCACAGCGGATCGCCATCG
GTACTACGCCAGGGCTGGAAGCCAAATAA

Upstream 100 bases:

>100_bases
TCATCGGCGGAATGAACCGCTGGTGCAATGAATGTTAGTTAACTGCCTGCCGTCAGGGGGCGATTAAACCTCATCTCGCT
TCATAGAAGGAGTTAATACT

Downstream 100 bases:

>100_bases
CCGACTGAGAAACAGTTAAATTTTCAGGGGGAAGTCCTAACGGGCTTCCCCCTTTGCTTTCTGTGGCGCAAGCCACAGTC
ACCCTTTTCTGCCTGTGCCA

Product: heat shock chaperone IbpB

Products: NA

Alternate protein names: 16 kDa heat shock protein B

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MRNYDLSPLLRQWIGFDKLASSMGGQEPQGFPPYNIEKSDDNHYRISLALAGFKQSELDIEVEGPRLTVRGKPTPSEKQV
EYLHQGLVCKEFALTFTLAEHLQVSEAQFENGLLHIDLVRQVPEALQPQRIAIGTTPGLEAK

Sequences:

>Translated_142_residues
MRNYDLSPLLRQWIGFDKLASSMGGQEPQGFPPYNIEKSDDNHYRISLALAGFKQSELDIEVEGPRLTVRGKPTPSEKQV
EYLHQGLVCKEFALTFTLAEHLQVSEAQFENGLLHIDLVRQVPEALQPQRIAIGTTPGLEAK
>Mature_142_residues
MRNYDLSPLLRQWIGFDKLASSMGGQEPQGFPPYNIEKSDDNHYRISLALAGFKQSELDIEVEGPRLTVRGKPTPSEKQV
EYLHQGLVCKEFALTFTLAEHLQVSEAQFENGLLHIDLVRQVPEALQPQRIAIGTTPGLEAK

Specific function: Associates with aggregated proteins, together with IbpA, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently ref

COG id: COG0071

COG function: function code O; Molecular chaperone (small heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small heat shock protein (HSP20) family

Homologues:

Organism=Escherichia coli, GI87082316, Length=142, Percent_Identity=60.5633802816901, Blast_Score=185, Evalue=9e-49,
Organism=Escherichia coli, GI1790122, Length=137, Percent_Identity=47.4452554744526, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: 686 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): IBPB_SERP5 (A8G7R9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476287.1
- ProteinModelPortal:   A8G7R9
- SMR:   A8G7R9
- STRING:   A8G7R9
- GeneID:   5606859
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0049
- eggNOG:   COG0071
- HOGENOM:   HBG748242
- OMA:   SFTLAEH
- ProtClustDB:   PRK11597
- BioCyc:   SPRO399741:SPRO_0049-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02001
- InterPro:   IPR002068
- InterPro:   IPR008978
- InterPro:   IPR022848

Pfam domain/function: PF00011 HSP20; SSF49764 HSP20_chap

EC number: NA

Molecular weight: Translated: 15944; Mature: 15944

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01031 HSP20

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNYDLSPLLRQWIGFDKLASSMGGQEPQGFPPYNIEKSDDNHYRISLALAGFKQSELDI
CCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEE
EVEGPRLTVRGKPTPSEKQVEYLHQGLVCKEFALTFTLAEHLQVSEAQFENGLLHIDLVR
EEECCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
QVPEALQPQRIAIGTTPGLEAK
HHHHHCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MRNYDLSPLLRQWIGFDKLASSMGGQEPQGFPPYNIEKSDDNHYRISLALAGFKQSELDI
CCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEE
EVEGPRLTVRGKPTPSEKQVEYLHQGLVCKEFALTFTLAEHLQVSEAQFENGLLHIDLVR
EEECCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
QVPEALQPQRIAIGTTPGLEAK
HHHHHCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA