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The map label for this gene is dnaA [H]

Identifier: 157368281

GI number: 157368281

Start: 30606

End: 31997

Strand: Direct

Name: dnaA [H]

Synonym: Spro_0032

Alternate gene names: 157368281

Gene position: 30606-31997 (Clockwise)

Preceding gene: 157368270

Following gene: 157368282

Centisome position: 0.56

GC content: 53.09

Gene sequence:

>1392_bases
GTGTCACTTTCGCTTTGGCAGCAGTGTCTTGCCCGATTGCAGGATGAGTTACCTGCCACAGAATTTAGTATGTGGATACG
CCCATTGCAGGCGGAACTGAGTGACAACACCCTGGCGCTTTATGCGCCCAATCGTTTTGTGCTGGATTGGGTTCGCGATA
AATACTTAAACAATATCAATGGTCTGTTGAATGATTTCTGCGGTACGGATGCCCCATTGCTGCGTTTTGAAGTGGGCAGT
AAGCCCCTCTCCCAAATCATCAGCCAGACCGTTACTGCCAGCGTCAGTGCACCCAGCGCGCCAATAGTGCGTGTTGCCGC
GCCGTCCCGCCCAAGCTGGGACAACGCAGCGCCGCAGCCTGAACTTTCTTACCGCTCCAACGTTAACCCAAAGCATACCT
TTGATAACTTTGTTGAGGGTAAATCCAACCAGTTGGCGCGCGCGGCGGCCCGTCAGGTGGCGGATAACCCGGGTGGAGCC
TATAACCCGTTGTTCCTTTATGGCGGCACCGGTCTGGGTAAAACTCACCTGTTGCACGCAGTGGGTAACGGCATCATGGC
ACGCAAGGCCAACGCCAAAGTGGTCTATATGCACTCTGAACGCTTTGTGCAGGACATGGTCAAAGCCTTGCAGAACAATG
CCATTGAAGAATTTAAGCGTTATTACCGTTCGGTCGATGCGCTGCTGATCGATGACATCCAATTTTTTGCCAATAAAGAG
CGTTCGCAGGAAGAGTTCTTCCATACCTTTAACGCCCTGTTGGAAGGCAATCAGCAGATTATCCTGACCTCGGACCGTTA
TCCGAAAGAGATTAACGGCGTGGAAGATCGTCTGAAATCCCGCTTCGGTTGGGGGCTGACGGTCGCGATCGAACCGCCGG
AGCTGGAAACCCGCGTAGCGATCCTGATGAAAAAGGCCGACGAGAATAATATCCGTCTGCCGGGCGAAGTGGCGTTCTTT
ATCGCCAAGCGCCTGCGTTCCAACGTGCGTGAGCTCGAAGGGGCGTTGAACCGCGTGATCGCCAACGCAAACTTTACCGG
CCGTGCCATTACCATTGATTTCGTACGTGAAGCGCTGCGCGATCTGCTGGCATTGCAGGAAAAGCTGGTCACCATCGATA
ATATTCAAAAGACGGTGGCGGAATACTATAAAATTAAAATCGCTGATTTGTTGTCCAAGCGGCGCTCGCGTTCCGTTGCC
CGTCCCCGCCAAATGGCGATGGCGTTGGCAAAAGAGCTCACCAACCACAGCCTGCCAGAAATCGGCGATGCGTTTGGTGG
CCGCGACCATACCACGGTGTTGCACGCCTGCCGGAAGATCGAGCAGTTGCGTGAGGAAAGTCACGACATCAAAGAAGATT
TCTCCAATTTAATCAGAACATTATCTTCCTAG

Upstream 100 bases:

>100_bases
CAGGCAAACAGCCGATGCCGGGTATGTGTCAAAAATCATGAGTTATAAAAAATTAGCCTGATTCTTTTCTTTTATTGATC
TTGTTCGAGTGGAGTCCGCC

Downstream 100 bases:

>100_bases
CGCTATGAAATTTATCGTTGAACGTGAGCATCTGCTAAAACCACTGCAACAGGTGAGCAGCCCGCTGGGCGGCCGCCCAA
CGCTGCCAATTCTGGGTAAC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTDAPLLRFEVGS
KPLSQIISQTVTASVSAPSAPIVRVAAPSRPSWDNAAPQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGA
YNPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKE
RSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENNIRLPGEVAFF
IAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKIADLLSKRRSRSVA
RPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Sequences:

>Translated_463_residues
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTDAPLLRFEVGS
KPLSQIISQTVTASVSAPSAPIVRVAAPSRPSWDNAAPQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGA
YNPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKE
RSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENNIRLPGEVAFF
IAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKIADLLSKRRSRSVA
RPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
>Mature_462_residues
SLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLNDFCGTDAPLLRFEVGSK
PLSQIISQTVTASVSAPSAPIVRVAAPSRPSWDNAAPQPELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGAY
NPLFLYGGTGLGKTHLLHAVGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKER
SQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENNIRLPGEVAFFI
AKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKIADLLSKRRSRSVAR
PRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=467, Percent_Identity=91.220556745182, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=232, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52210; Mature: 52079

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
GLLNDFCGTDAPLLRFEVGSKPLSQIISQTVTASVSAPSAPIVRVAAPSRPSWDNAAPQP
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCC
ELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHA
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
VGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKE
HCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVA
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
ILMKKADENNIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
DLLALQEKLVTIDNIQKTVAEYYKIKIADLLSKRRSRSVARPRQMAMALAKELTNHSLPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
IGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
GLLNDFCGTDAPLLRFEVGSKPLSQIISQTVTASVSAPSAPIVRVAAPSRPSWDNAAPQP
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCC
ELSYRSNVNPKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHA
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
VGNGIMARKANAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFANKE
HCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVA
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
ILMKKADENNIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
DLLALQEKLVTIDNIQKTVAEYYKIKIADLLSKRRSRSVARPRQMAMALAKELTNHSLPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
IGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA