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The map label for this gene is eptB

Identifier: 157163021

GI number: 157163021

Start: 3738087

End: 3739778

Strand: Reverse

Name: eptB

Synonym: EcHS_A3747

Alternate gene names: 157163021

Gene position: 3739778-3738087 (Counterclockwise)

Preceding gene: 157163022

Following gene: 157163019

Centisome position: 80.54

GC content: 50.41

Gene sequence:

>1692_bases
ATGAGATACATCAAATCGATTACACAGCAGAAGCTGAGCTTTTTGCTTGCAATCTATATTGGCCTTTTTATGAATGGCGC
GGTTTTTTACCGCCGCTTCGGCAGCTATGCGCACGATTTTACCGTCTGGAAAGGCATTTCTGCTGTTGTTGAACTGGCCG
CCACCGTACTGGTGACCTTCTTTTTACTACGTCTTCTTTCGCTGTTTGGCCGCCGCAGCTGGCGTATTCTGGCATCGCTG
GTGGTGCTCTTTTCCGCAGGTGCCAGCTATTACATGACCTTCCTTAATGTGGTCATTGGTTATGGCATCATCGCTTCCGT
CATGACCACCGATATCGACCTGTCAAAAGAAGTTGTTGGTCTGAACTTTATTCTCTGGTTAATCGCCGTTAGTGCATTGC
CTCTTATCCTTATCTGGAATAACCGCTGTCGCTACACCTTGCTCCGACAACTGCGAACCCCAGGGCAGCGTATTCGCAGC
CTGGCGGTCGTCGTACTGGCGGGTATTATGGTTTGGGCACCGATTCGTTTGCTGGATATCCAGCAGAAGAAAGTGGAGAG
GGCGACCGGCGTTGATTTGCCGAGTTATGGCGGTGTCGTAGCGAACTCTTATCTGCCATCAAACTGGCTTTCTGCGTTGG
GGCTGTATGCCTGGGCGCGGGTCGATGAATCTTCCGATAATAATTCATTGCTTAATCCGGCGAAGAAATTCACTTATCAG
GCACCGCAAAACGTTGATGACACTTATGTCGTGTTTATCATCGGTGAAACCACGCGTTGGGACCATATGGGTATTTTCGG
CTATGAGCGTAATACCACGCCGAAACTGGCCCAGGAGAAAAATCTGGCGGCGTTCCGTGGTTACTCCTGTGATACCGCAA
CCAAACTCTCACTGCGTTGCATGTTTGTACGTCAGGGGGGCGCGGAAGATAATCCGCAGCGCACATTAAAAGAACAGAAC
ATTTTCGCGGTTCTGAAGCAGTTAGGATTCAGTTCTGACCTCTACGCTATGCAGAGCGAAATGTGGTTCTACAGCAACAC
GATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCGGAGCCACGTAATCGTGGCAAGCCGGTAGATGATATGTTGC
TGGTAGACGAAATGCAGCAATCGCTAGGGCGCAACCCGGATGGTAAGCATCTGATCATTCTGCATACCAAAGGTTCGCAT
TTTAACTACACCCAGCGTTATCCGCGTAGCTTCGCGCAGTGGAAGCCGGAATGTATTGGTGTTGATAGCGGCTGTACCAA
AGCGCAGATGATCAACTCCTATGACAACTCGGTGACCTATGTGGATCACTTTATCTCCAGCGTGATTGATCAGGTTCGCG
ATAAGAAAGCGATTGTGTTCTACGCAGCTGACCACGGTGAGTCAATTAATGAACGCGAGCACCTGCACGGCACGCCGCGT
GAACTGGCACCGCCGGAGCAGTTCCGCGTACCGATGATGGTCTGGATGTCAGATAAATATCTGGAAAATCCGGCCAATGC
GCAGGCGTTTGCGCAGCTGAAAAAAGAAGCCGACATGAAAGTGCCACGCCGTCACGTAGAGCTGTACGATACCATCATGG
GTTGTCTTGGCTATACTTCACCGGATGGTGGAATTAACGAAAACAACAACTGGTGTCACATCCCGCAGGCAAAAGAGGCA
GCGGCTAACTAA

Upstream 100 bases:

>100_bases
GTAGATTTTACTTATCTGACTACCTCCGCACTTTTTCCCTGCCGGGCCTGAAAAGCCACTAAGCAGGGTGTTATCACCTG
TTTGTCCAGGGTTTGTTTGC

Downstream 100 bases:

>100_bases
GCGGCCTGCTGACTTTCTCGCCGATCAAAAGGCATTTTGCTATTAAGGGATTGACGAGGGCGTATCTGCGCAGTAAGATG
CGCCCCGCATTCGGTGATTG

Product: phosphoethanolamine transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTFFLLRLLSLFGRRSWRILASL
VVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQN
IFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSH
FNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEA
AAN

Sequences:

>Translated_563_residues
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTFFLLRLLSLFGRRSWRILASL
VVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQN
IFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSH
FNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEA
AAN
>Mature_563_residues
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTFFLLRLLSLFGRRSWRILASL
VVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQN
IFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSH
FNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEA
AAN

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily

Homologues:

Organism=Escherichia coli, GI87082286, Length=563, Percent_Identity=100, Blast_Score=1178, Evalue=0.0,
Organism=Escherichia coli, GI87082372, Length=523, Percent_Identity=24.4741873804971, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87082223, Length=257, Percent_Identity=31.9066147859922, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790392, Length=267, Percent_Identity=25.4681647940075, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): EPTB_ECOLI (P37661)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D65153
- RefSeq:   AP_004248.1
- RefSeq:   NP_418002.2
- ProteinModelPortal:   P37661
- STRING:   P37661
- EnsemblBacteria:   EBESCT00000003951
- EnsemblBacteria:   EBESCT00000016056
- GeneID:   948068
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5660
- KEGG:   eco:b3546
- EchoBASE:   EB2176
- EcoGene:   EG12267
- eggNOG:   COG2194
- GeneTree:   EBGT00050000009020
- HOGENOM:   HBG417174
- OMA:   CMRYIKS
- ProtClustDB:   PRK11560
- BioCyc:   EcoCyc:EG12267-MONOMER
- Genevestigator:   P37661
- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917
- Gene3D:   G3DSA:3.40.720.10

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase; SSF53649 Alkaline_phosphatase_core

EC number: NA

Molecular weight: Translated: 63805; Mature: 63805

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17cbfd7c)-; HASH(0x17ed5598)-; HASH(0x1833ba58)-; HASH(0x1797dd1c)-; HASH(0x1797a52c)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVG
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRSLAVVVLAGIMVWAPIRLLDI
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
HHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCC
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRC
CCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHCCCHHHCCCCCCCCHHEEEEE
MFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGA
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCC
EPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSHFNYTQRYPRSFAQWKPECIG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEC
VDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCH
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTS
HCCCHHHHCCCEEEEECHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PDGGINENNNWCHIPQAKEAAAN
CCCCCCCCCCEEECCCHHHHCCH
>Mature Secondary Structure
MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWKGISAVVELAATVLVTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYGIIASVMTTDIDLSKEVVG
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRSLAVVVLAGIMVWAPIRLLDI
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAWARVDESSDNNSLLNPAKKFTYQ
HHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCC
APQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKLAQEKNLAAFRGYSCDTATKLSLRC
CCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHCCCHHHCCCCCCCCHHEEEEE
MFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDLYAMQSEMWFYSNTMADNIAYREQIGA
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCC
EPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLIILHTKGSHFNYTQRYPRSFAQWKPECIG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEC
VDSGCTKAQMINSYDNSVTYVDHFISSVIDQVRDKKAIVFYAADHGESINEREHLHGTPR
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCH
ELAPPEQFRVPMMVWMSDKYLENPANAQAFAQLKKEADMKVPRRHVELYDTIMGCLGYTS
HCCCHHHHCCCEEEEECHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PDGGINENNNWCHIPQAKEAAAN
CCCCCCCCCCEEECCCHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646