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The map label for this gene is pflB1

Identifier: 157162596

GI number: 157162596

Start: 3308962

End: 3311256

Strand: Reverse

Name: pflB1

Synonym: EcHS_A3302

Alternate gene names: 157162596

Gene position: 3311256-3308962 (Counterclockwise)

Preceding gene: 157162597

Following gene: 157162595

Centisome position: 71.31

GC content: 53.12

Gene sequence:

>2295_bases
ATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGA
AATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGCGACGCCTG
CCACCACGGAATTGTGGGAAAAAGTAATGGAAGGCATCCGTATCGAAAATGCAACCCACGCGCCGGTTGATTTCGATACC
AATATTGCCACCACAATTACCGCTCATGATGCGGGATATATTAACCAGCCGCTGGAAAAAATTGTTGGCCTGCAAACGGA
TGCGCCGTTGAAACGTGCGCTACACCCGTTCGGTGGCATTAATATGATTAAAAGTTCATTCCACGCCTATGGCCGAGAAA
TGGACAGTGAATTTGAATATCTGTTTACCGATCTGCGTAAAACCCATAACCAGGGCGTATTTGATGTTTACTCACCGGAT
ATGCTGCGCTGCCGTAAATCTGGCGTGCTGACCGGTTTACCAGATGGCTATGGCCGTGGGCGCATTATCGGTGACTATCG
CCGCGTAGCGCTGTATGGCATCAGTTATCTGGTACGTGAACGCGAACTGCAATTTGCCGATCTCCAGTCTCGTCTGGAAA
AAGGCGAGGATCTGGAAGCCACCATCCGTCTGCGTGAGGAGCTGGCAGAGCATCGTCATGCGCTGTTGCAGATTCAGGAA
ATGGCGGCGAAATATGGCTTTGATATCTCTCGCCCGGCGCAGAATGCGCAGGAAGCGGTGCAGTGGCTCTACTTCGCTTA
TCTGGCGGCAGTGAAATCGCAAAATGGCGGCGCGATGTCGCTGGGCCGCACGGCATCGTTCCTCGATATCTACATTGAGC
GCGACTTTAAAGCTGGCGTACTCAATGAGCAGCAGGCACAGGAACTGATCGATCACTTCATCATGAAGATCCGTATGGTA
CGCTTCCTGCGTACACCGGAATTTGATTCGCTGTTCTCCGGCGACCCAATCTGGGCGACGGAAGTGATCGGCGGGATGGG
GCTGGACGGTCGTACGCTGGTGACCAAAAACTCCTTCCGCTATTTGCACACCCTGCACACTATGGGGCCGGCACCGGAAC
CTAACCTGACCATTCTTTGGTCGGAAGAATTACCGATTGCCTTCAAAAAATATGCCGCGCAGGTGTCGATCGTCACCTCT
TCCTTGCAGTATGAAAATGACGATCTGATGCGTACTGACTTCAACAGCGACGATTACGCGATTGCCTGCTGCGTCAGCCC
AATGGTGATTGGTAAGCAAATGCAGTTCTTTGGTGCACGCGCTAACCTGGCGAAAACGCTGCTCTACGCAATTAACGGCG
GGGTGGACGAGAAGCTGAAGATTCAGGTCGGGCCGAAAACAGCACCGCTGATGGACGACGTGCTGGATTACGACAAAGTG
ATGGACAGCCTCGATCACTTCATGGACTGGCTGGCGGTGCAGTACATCAGCGCGCTGAATATCATTCACTACATGCACGA
CAAGTACAGCTACGAAGCTTCGCTGATGGCGCTGCACGATCGTGATGTCTATCGCACTATGGCATGCGGCATCGCGGGCC
TGTCGGTGGCGACGGACTCCCTGTCTGCCATCAAATATGCCCGCGTGAAACCAATCCGTGACGAAAACGGCCTGGCGGTG
GACTTTGAAATCGACGGTGAATATCCGCAGTACGGCAACAACGACGAGCGCGTAGACAGCATTGCCTGCGACCTGGTTGA
ACGCTTTATGAAGAAAATTAAAGCGCTGCCAACCTATCGCAACGCCGTCCCTACCCAGTCGATTCTGACTATCACTTCTA
ACGTGGTGTACGGCCAGAAAACCGGTAATACGCCGGACGGTCGTCGCGCCGGAACACCGTTCGCGCCGGGCGCTAACCCG
ATGCATGGTCGTGACCGCAAAGGTGCCGTGGCCTCATTGACGTCGGTGGCGAAACTGCCGTTCACCTACGCCAAAGATGG
GATCTCGTACACCTTCTCAATCGTTCCTGCGGCGCTGGGCAAAGAAGATCCAGTACGTAAAACCAACCTTGTCGGCCTGC
TGGATGGGTATTTCCACCACGAAGCGGATGTCGAAGGCGGTCAACACCTCAACGTCAACGTAATGAATCGGGAAATGCTG
CTGGATGCCATCGAGCACCCGGAAAAATATCCTAACCTGACAATCCGTGTCTCTGGCTACGCCGTGCGCTTCAACGCACT
GACCCGTGAACAGCAACAGGATGTTATTTCACGTACCTTTACCCAGGCGCTCTGA

Upstream 100 bases:

>100_bases
AGAAAAAATGATTGCTTTGGATGCCATTCATTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTA
TTGTAGAGAGATTATTTTTC

Downstream 100 bases:

>100_bases
CGCCGGAGGATGTATGAAAAAGATTATCGAAACGCAACGTGCCCCAGGCGCAATCGGCCCTTATGTTCAGGGCGTTGATT
TAGGCAGCATGGTCTTCACC

Product: formate acetyltransferase

Products: CoA; pyruvate

Alternate protein names: Keto-acid formate-lyase

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRHALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL

Sequences:

>Translated_764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRHALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
>Mature_764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRHALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain

Homologues:

Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=100, Blast_Score=1592, Evalue=0.0,
Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=79.5212765957447, Blast_Score=1292, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=574, Percent_Identity=28.397212543554, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790388, Length=641, Percent_Identity=25.8970358814353, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=76.5625, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TDCE_ECOLI (P42632)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G65100
- RefSeq:   AP_003661.1
- RefSeq:   YP_026205.1
- ProteinModelPortal:   P42632
- SMR:   P42632
- DIP:   DIP-10972N
- MINT:   MINT-1231745
- STRING:   P42632
- EnsemblBacteria:   EBESCT00000002574
- EnsemblBacteria:   EBESCT00000016306
- GeneID:   947623
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5522
- KEGG:   eco:b3114
- EchoBASE:   EB2612
- EcoGene:   EG12758
- eggNOG:   COG1882
- GeneTree:   EBGT00050000009047
- HOGENOM:   HBG285825
- OMA:   EAYGREM
- ProtClustDB:   CLSK869953
- BioCyc:   EcoCyc:KETOBUTFORMLY-INACT-MONOMER
- BioCyc:   MetaCyc:KETOBUTFORMLY-INACT-MONOMER
- Genevestigator:   P42632
- GO:   GO:0005737
- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- PIRSF:   PIRSF000379
- TIGRFAMs:   TIGR01255

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL

EC number: 2.3.1.54

Molecular weight: Translated: 85937; Mature: 85937

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: ACT_SITE 423-423 ACT_SITE 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWE
CEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHH
KVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCH
NMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRHALLQIQE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEECCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYR
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHH
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWE
CEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHH
KVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCH
NMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRHALLQIQE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEECCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYR
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHH
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901