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The map label for this gene is norW [H]

Identifier: 157162157

GI number: 157162157

Start: 2851330

End: 2852463

Strand: Direct

Name: norW [H]

Synonym: EcHS_A2847

Alternate gene names: 157162157

Gene position: 2851330-2852463 (Clockwise)

Preceding gene: 157162156

Following gene: 157162161

Centisome position: 61.4

GC content: 54.14

Gene sequence:

>1134_bases
ATGAGCAACGGCATTGTGATCATTGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCAG
TATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGC
AACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGG
GTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAAC
CGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCT
GTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTT
TGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGCCAGTATTCTGGCGTCGTTAATGCCACCGGAAGTAAGCAG
CCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGTTGAAATCTCAGTTACAGGGGCTGGAAAAAACGGATT
CTGGCATTCTGGCAACGCTGGACCGCCAGCGTAGCATCGAAGTGGATGCGGTAATTGCCGCCACCGGACTGCGCCCGGAA
ACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGA
TATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGG
TGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTA
CCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCG
CGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAA
CATTGCCGATGTAG

Upstream 100 bases:

>100_bases
TTGCGCCAGGAACGCCGTGGAGTGAAGTTCCGGATAACTTCCTCTGCCCGGAATGCTCTCTCGGCAAAGACGTCTTTGAC
GAACTGGCATCGGAGGCAAA

Downstream 100 bases:

>100_bases
GTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAT
AAGGCGTTTACGCCGCATCC

Product: nitric oxide reductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: Flavorubredoxin reductase; FlRd-reductase; FlavoRb reductase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTW
VTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM

Sequences:

>Translated_377_residues
MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTW
VTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM
>Mature_376_residues
SNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWV
TDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFC
RAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPET
ALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELP
LHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM

Specific function: One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI65787454, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI226437568, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4757732, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI22202629, Length=209, Percent_Identity=26.7942583732057, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789065, Length=377, Percent_Identity=99.2042440318302, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1788892, Length=274, Percent_Identity=27.7372262773723, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=25.6965944272446, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787352, Length=277, Percent_Identity=28.8808664259928, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=276, Percent_Identity=25.7246376811594, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24639257, Length=373, Percent_Identity=22.7882037533512, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281359715, Length=373, Percent_Identity=22.7882037533512, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281359713, Length=373, Percent_Identity=22.7882037533512, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24639250, Length=373, Percent_Identity=22.7882037533512, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI18543267, Length=373, Percent_Identity=22.7882037533512, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24639252, Length=373, Percent_Identity=22.7882037533512, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24585130, Length=372, Percent_Identity=21.7741935483871, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 41348; Mature: 41217

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHHHHCCCEECCHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
HHHHHHCCCEEECCCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
CCCCCCEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEECCCCCHHHHHHHHH
SEDRMKEAFGLLKTLPM
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
CCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHHHHCCCEECCHHHCCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
HHHHHHCCCEEECCCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDADQLRAFVV
CCCCCCEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEECCCCCHHHHHHHHH
SEDRMKEAFGLLKTLPM
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA