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The map label for this gene is hyfH [H]

Identifier: 157161948

GI number: 157161948

Start: 2632107

End: 2632646

Strand: Direct

Name: hyfH [H]

Synonym: EcHS_A2622

Alternate gene names: 157161948

Gene position: 2632107-2632646 (Clockwise)

Preceding gene: 157161947

Following gene: 157161949

Centisome position: 56.68

GC content: 55.19

Gene sequence:

>540_bases
ATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCC
AGGCTTTCGCGGGAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGCGCCTGTGCTTGTCCGGCAAATGCGCTGA
CTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGAGGCGTTGTATTTACTGCGGACGTTGTGAA
GAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCG
CGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCAGCAAAAAACCGTCGCACTGGCTACTGAAT
TGTTGGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTATGGGCGCAAGCGAGCGTCTGCCCGGAGTGCAAACAA
CGCGCGACGCTGCTCAACGACGATACAGATGTACCGCTGGTGGCTAAGGAGCAGCTATGA

Upstream 100 bases:

>100_bases
GGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGC
CGCTGAAGTAAGGACAGAAG

Downstream 100 bases:

>100_bases
GCCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCAAAAGATGAAGCAGCATTTGCTACAG
GATATCCGTCGCTCGGCTTA

Product: hydrogenase 4 subunit H

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCE
EVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQ
RATLLNDDTDVPLVAKEQL

Sequences:

>Translated_179_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCE
EVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQ
RATLLNDDTDVPLVAKEQL
>Mature_179_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCE
EVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQ
RATLLNDDTDVPLVAKEQL

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=106, Percent_Identity=40.5660377358491, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1788833, Length=181, Percent_Identity=93.9226519337017, Blast_Score=319, Evalue=7e-89,
Organism=Escherichia coli, GI1789075, Length=170, Percent_Identity=44.1176470588235, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1788617, Length=102, Percent_Identity=38.2352941176471, Blast_Score=70, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=106, Percent_Identity=39.622641509434, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864306, Length=106, Percent_Identity=41.5094339622642, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20115; Mature: 20115

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
6.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACPANALTIQTDDQ
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCC
QNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCER
CCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCC
PFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQRATLLNDDTDVPLVAKEQL
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCC
>Mature Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACPANALTIQTDDQ
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCC
QNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCER
CCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHCC
PFAQQKTVALATELLAQQQNAPQNREMLWAQASVCPECKQRATLLNDDTDVPLVAKEQL
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]