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The map label for this gene is pheS

Identifier: 157161175

GI number: 157161175

Start: 1814760

End: 1815743

Strand: Reverse

Name: pheS

Synonym: EcHS_A1794

Alternate gene names: 157161175

Gene position: 1815743-1814760 (Counterclockwise)

Preceding gene: 157161176

Following gene: 157161174

Centisome position: 39.1

GC content: 53.86

Gene sequence:

>984_bases
ATGTCACATCTCGCAGAACTGGTTGCCAGTGCGAAGGCGGCCATTAGCCAGGCGTCAGATGTTGCCGCGTTAGATAATGT
GCGCGTCGAATATTTGGGTAAAAAAGGGCACTTAACCCTTCAGATGACGACCCTGCGTGAGCTGCCGCCAGAAGAGCGTC
CGGCAGCTGGTGCGGTTATCAACGAAGCGAAAGAGCAGGTTCAGCAGGCGCTGAATGCGCGTAAAGCGGAACTGGAAAGC
GCTGCACTGAATGCGCGTCTGGCGGCGGAAACGATTGATGTCTCTCTGCCAGGTCGTCGCATTGAAAACGGCGGTCTGCA
TCCGGTTACCCGTACCATCGACCGTATCGAAAGTTTCTTCGGTGAGCTTGGCTTTACCGTGGCAACCGGGCCGGAAATCG
AAGACGATTATCATAACTTCGATGCTCTGAACATTCCTGGTCACCACCCGGCGCGCGCTGACCACGACACTTTCTGGTTT
GACACTACCCGCCTGCTGCGTACCCAGACCTCTGGCGTACAGATCCGCACCATGAAAGCCCAGCAGCCACCGATTCGTAT
CATCGCGCCTGGCCGTGTTTATCGTAACGACTACGACCAGACTCACACGCCGATGTTCCATCAGATGGAAGGTCTGATTG
TTGATACCAACATCAGCTTTACCAACCTGAAAGGCACGCTGCACGACTTCCTGCGTAACTTCTTTGAGGAAGATTTGCAG
ATTCGCTTCCGTCCTTCCTACTTCCCGTTTACCGAACCTTCTGCAGAAGTGGACGTCATGGGTAAAAACGGTAAATGGCT
GGAAGTGCTGGGCTGCGGGATGGTGCATCCGAACGTGTTGCGTAACGTTGGCATCGACCCCGAAGTTTACTCTGGTTTCG
CCTTCGGGATGGGGATGGAGCGTCTGACTATGTTGCGTTACGGCGTCACCGACCTGCGTTCATTCTTCGAAAACGATCTG
CGTTTCCTCAAACAGTTTAAATAA

Upstream 100 bases:

>100_bases
TCGAGCACAGCCCGGGACCAAAATGGCAAGTAAAATAGCCTGATGGGATAGGCTCTAAGTCCAACGAACCAGTGTCACCA
CTGACACAATGAGGAAAACC

Downstream 100 bases:

>100_bases
GGCAGGAATAGATTATGAAATTCAGTGAACTGTGGTTACGCGAATGGGTGAACCCGGCGATTGATAGCGATGCGCTGGCA
AATCAAATCACTATGGCGGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELES
AALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELES
AALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDL
RFLKQFK
>Mature_326_residues
SHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELESA
ALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFD
TTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQI
RFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLR
FLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=318, Percent_Identity=29.874213836478, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4758340, Length=261, Percent_Identity=32.9501915708812, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=100, Blast_Score=678, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=31.095406360424, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=30.7116104868914, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=40, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI18858079, Length=272, Percent_Identity=30.5147058823529, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17137424, Length=237, Percent_Identity=29.1139240506329, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ECO24 (A7ZMI2)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463011.1
- ProteinModelPortal:   A7ZMI2
- SMR:   A7ZMI2
- STRING:   A7ZMI2
- EnsemblBacteria:   EBESCT00000021293
- GeneID:   5589852
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1933
- eggNOG:   COG0016
- GeneTree:   EBGT00050000011266
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   ECOL331111:ECE24377A_1933-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 36832; Mature: 36701

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVI
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCHHHH
NEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDTTRLLRTQTSGVQIRTMKA
HHCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEC
QQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
CCCCEEEEECCHHHCCCCCCCCCCHHHHHCCEEEECCCCEECCHHHHHHHHHHHHHHCCE
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGME
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHHCCHHHHCCHH
RLTMLRYGVTDLRSFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVI
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCHHHH
NEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDTTRLLRTQTSGVQIRTMKA
HHCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEEC
QQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
CCCCEEEEECCHHHCCCCCCCCCCHHHHHCCEEEECCCCEECCHHHHHHHHHHHHHHCCE
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVGIDPEVYSGFAFGMGME
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHHCCHHHHCCHH
RLTMLRYGVTDLRSFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA