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The map label for this gene is pheT [H]

Identifier: 157161174

GI number: 157161174

Start: 1812358

End: 1814745

Strand: Reverse

Name: pheT [H]

Synonym: EcHS_A1793

Alternate gene names: 157161174

Gene position: 1814745-1812358 (Counterclockwise)

Preceding gene: 157161175

Following gene: 157161173

Centisome position: 39.08

GC content: 54.36

Gene sequence:

>2388_bases
ATGAAATTCAGTGAACTGTGGTTACGCGAATGGGTGAACCCGGCGATTGATAGCGATGCGCTGGCAAATCAAATCACTAT
GGCGGGCCTGGAAGTTGACGGTGTAGAACCGGTTGCCGGCAGCTTCCACGGCGTGGTCGTTGGTGAAGTGGTTGAGTGTG
CTCAGCATCCGAACGCTGACAAACTGCGTGTGACAAAAGTGAATGTCGGCGGCGATCGCCTGCTGGACATCGTCTGCGGT
GCGCCAAACTGCCGTCAGGGCCTGCGTGTGGCGGTAGCGACCATTGGTGCTGTTCTGCCGGGTGATTTCAAAATTAAAGC
GGCGAAACTGCGTGGCGAACCGTCTGAAGGGATGCTGTGCTCCTTCTCTGAACTGGGCATTTCTGACGATCACAGCGGCA
TTATCGAACTGCCTGCGGATGCGCCGATTGGCACCGATATCCGTGAATACCTGAAACTTGATGACAACACCATCGAAATC
AGCGTGACGCCAAACCGTGCCGACTGCTTAGGCATCATTGGTGTTGCGCGTGACGTTGCCGTGCTGAACCAGCTGCCGCT
GGTTCAACCGGAAATCGTTCCGGTTGGTGCGACCATCGACGACACGCTGCCGATTACAGTCGAAGCGCCGGAAGCCTGCC
CGCGTTATCTTGGCCGTGTGGTAAAAGGCATTAACGTTAAAGCGCCAACTCCGCTGTGGATGAAAGAAAAACTGCGTCGT
TGCGGGATCCGTTCTATCGATGCAGTTGTTGACGTCACCAACTATGTGCTGCTCGAACTGGGCCAGCCGATGCACGCTTT
CGATAAAGATCGCATTGAAGGCGGCATTGTGGTGCGGATGGCGAAAGAGGGCGAAACGCTGGTGCTGCTCGACGGTACTG
AAGCGAAGCTGAATGCTGACACTCTGGTCATCGCCGACCACAACAAGGCGCTGGCGATGGGCGGCATCTTCGGTGGCGAA
CACTCTGGCGTGAATGACGAAACACAAAACGTGCTGCTGGAATGCGCGTTCTTTAGCCCGCTGTCTATCACCGGTCGTGC
TCGTCGTCATGGCCTGCATACCGATGCGTCTCACCGTTATGAGCGTGGCGTTGATCCGGCACTGCAGCACAAAGCGATGG
AACGTGCGACCCGTCTGCTGATCGACATCTGCGGTGGTGAGGCTGGCCCGGTAATTGATATCACCAACGAAGCAACGCTG
CCGAAGCGTGCAACCATCACTCTACGTCGTAGCAAACTGGATCGCCTGATCGGCCATCATATTGCGGATGAGCAGGTAAC
TGACATTCTGCGTCGTCTCGGCTGCGAAGTGACCGAAGGCAAAGACGAATGGCAGGCAGTTGCGCCGAGCTGGCGTTTCG
ATATGGAGATTGAAGAAGATCTGGTTGAAGAAGTCGCGCGTGTTTACGGCTACAACAACATCCCGGATGAGCCGGTACAG
GCAAGCCTGATTATGGGTACTCACCGTGAAGCTGACCTGTCGCTCAAGCGCGTGAAAACGCTGCTCAACGACAAAGGCTA
TCAGGAAGTGATCACCTACAGCTTCGTTGATCCGAAAGTGCAGCAGATGATCCATCCAGGCGTTGAAGCCTTACTGCTGC
CAAGCCCGATCTCTGTTGAAATGTCAGCAATGCGTCTTTCTCTGTGGACTGGCCTGCTGGCAACCGTGGTGTACAACCAG
AACCGTCAGCAGAACCGTGTGCGCATTTTCGAAAGCGGTCTGCGTTTCGTACCAGATACTCAGGCACCGTTGGGCATTCG
TCAGGATCTGATGTTAGCCGGTGTGATTTGCGGTAACCGTTACGAAGAGCACTGGAACCTGGCAAAAGAGACCGTTGATT
TCTATGATTTGAAAGGCGATCTTGAATCCGTTCTCGACCTGACCGGTAAACTGAATGAGGTTGAGTTCCGTGCAGAAGCG
AATCCGGCACTGCATCCGGGGCAATCCGCAGCGATTTATCTGAAAGGTGAACGTATTGGTTTTGTTGGGGTTGTTCATCC
TGAACTGGAACGTAAACTGGATCTTAACGGTCGCACTCTGGTGTTCGAACTGGAGTGGAACAAGCTCGCAGACCGCGTGG
TGCCTCAGGCGCGCGAGATTTCTCGCTTCCCGGCGAACCGTCGTGACATCGCGGTGGTGGTCGCAGAAAACGTTCCCGCA
GCGGATATTTTATCCGAATGTAAGAAAGTTGGCGTAAATCAGGTAGTTGGCGTAAACTTATTTGACGTGTACCGCGGTAA
GGGTGTTGCGGAGGGGTATAAGAGCCTCGCCATAAGCCTGATCCTGCAAGATACCAGCCGTACACTCGAAGAAGAGGAGA
TTGCCGCTACCGTCGCCAAATGTGTAGAGGCATTAAAAGAGCGATTCCAGGCATCATTGAGGGATTGA

Upstream 100 bases:

>100_bases
AGCGTCTGACTATGTTGCGTTACGGCGTCACCGACCTGCGTTCATTCTTCGAAAACGATCTGCGTTTCCTCAAACAGTTT
AAATAAGGCAGGAATAGATT

Downstream 100 bases:

>100_bases
ACCTATGACGCTTACAAAAGCTGAAATGTCAGAATATCTGTTTGATAAGCTTGGGCTTAGCAAGCGGGATGCCAAAGAAC
TGGTTGAACTGTTTTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCG
APNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGE
HSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATL
PKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQ
NRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEA
NPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=100, Blast_Score=1619, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=388, Percent_Identity=22.9381443298969, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87379; Mature: 87379

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNAD
CCHHHHHHHHHCCCCCCCHHHHHHHEEECEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVTPNRADCLGIIGVARDVA
EHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
HHHCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRY
EEEEEECCCEEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCHHHHCCCCCCCHHHH
ERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
IADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
CCHHHHHHHHHHHCCEECCCCHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVE
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCCEEEECCCCCCH
MSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
YEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEE
FVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCH
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAK
HHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHEEHEEEECHHHHHHHHHHHHHHHH
CVEALKERFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNAD
CCHHHHHHHHHCCCCCCCHHHHHHHEEECEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
KLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
CEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
SFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVTPNRADCLGIIGVARDVA
EHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHH
VLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
HHHCCCCCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNAD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRY
EEEEEECCCEEEECCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCHHHHCCCCCCCHHHH
ERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHHHHH
IADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
CCHHHHHHHHHHHCCEECCCCHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCE
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVE
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCHHHHHHHCCCCCEEEECCCCCCH
MSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
YEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCEEE
FVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCH
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAK
HHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHEEHEEEECHHHHHHHHHHHHHHHH
CVEALKERFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]