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The map label for this gene is chbA [H]

Identifier: 157161004

GI number: 157161004

Start: 1640277

End: 1640588

Strand: Reverse

Name: chbA [H]

Synonym: EcHS_A1618

Alternate gene names: 157161004

Gene position: 1640588-1640277 (Counterclockwise)

Preceding gene: 157161005

Following gene: 157161003

Centisome position: 35.33

GC content: 43.91

Gene sequence:

>312_bases
ATGTTTGCAGATGAAGAACTTGTTATGGAGTTACTGGTCAACGCGGGGCAGGCACGTTCTGACGCGATGGAAGCCATACG
TTGTGCCGGGCAAAAAGACTGGCAGGGCGCAACTCAGCTAATGGCCAGTTCTGAATCTGCCTGTCTTCAGGCACATAAGA
TTCAGACCGCATTAATTAGTCAGGATGAAGGCTGTGGCAAAATAAAAGTTAATTTAATTCTGGTTCATGCACAGGATCAT
TTGATGAATGCAATATTATGCCAGGATTTAGCCAGGGAAATTATTTCATTAAGAAAAGAACTCCATGCCTGA

Upstream 100 bases:

>100_bases
TGTGGCGACAATGATCTATTTACCTTTTGTTATTGCCGCCAACAAAGTCAATAACATGACAACTAACGGATAATTCCATT
TACCCAGGAAGGAGATTGTG

Downstream 100 bases:

>100_bases
TTTTTTCCTCCAGTGCGATAGCTGGAGGAAATTATTATACTCTTAAGGATAAGTCATGAATATAAAGACGCTTAACATCA
GCCTTTTGTCTTTTTCTATT

Product: PTS system lactose/cellobiose-specific family IIA subunit

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Chb; EIII-Chb; PTS system N,N'-diacetylchitobiose-specific EIIA component [H]

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDH
LMNAILCQDLAREIISLRKELHA

Sequences:

>Translated_103_residues
MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDH
LMNAILCQDLAREIISLRKELHA
>Mature_103_residues
MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDH
LMNAILCQDLAREIISLRKELHA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=99, Percent_Identity=49.4949494949495, Blast_Score=93, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 11302; Mature: 11302

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALIS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
QDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELHA
CCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALIS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHC
QDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELHA
CCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]