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The map label for this gene is celA [H]

Identifier: 157161003

GI number: 157161003

Start: 1637085

End: 1638524

Strand: Reverse

Name: celA [H]

Synonym: EcHS_A1615

Alternate gene names: 157161003

Gene position: 1638524-1637085 (Counterclockwise)

Preceding gene: 157161004

Following gene: 157161001

Centisome position: 35.29

GC content: 44.93

Gene sequence:

>1440_bases
ATGTCAGGATTTAAAAAAGGTTTTTTATGGGGCGGCGCGGTAGCCGCGCATCAGTTGGAAGGTGGCTGGAATGAAGGAGG
AAAAGGCATCAGTATCGCTGATGTGATGACTGCTGTCGCTCACGGGGTGCCGCGTGAAGTGACAGAAGGCGTTATCGACG
GGCTTAATTATCCCAATCATGAAGCAATTGATTTTTATCATCGCTATAAAACAGATATTCAGTTATTTGCCGAGATGGGA
TTCAAATGCTTTCGAACTTCCATTGCCTGGACACGAATCTTTCCGCAAGGTGACGAACAGGAGCCGAATGAAGAGGGTTT
ACAATTTTATGATGATCTGTTCGATGAATGCCTTAAGCAGGGAATGGAACCTGTGGTGACGCTTTCACATTTTGAGATGC
CTTATCATCTGGTGACAAAATATGGTGGCTGGCGAAACCGTAAACTGATCGACTTTTTTATCCGCTTCGCATCAACGGTC
TTCACGCGCTATAAAGAAAAAGTAAAGTACTGGATGACGTTTAACGAAATCAATAATCAGGTGAATTTCAGCGAAAGCCT
GTGTCCATTTACTAATTCCGGTATCTTGTATTCGCCAGAGGAAGATATCAATGAGCGCGAACAAATAATGTACCAGGCGG
TACATTACGAGTTAGTTGCCAGTGCCCTGGCGGTACAGACTGGAAAATCGATTAATCCTGAATTTAATATCGGCTGCATG
ATCGCCATGTGCCCCATCTATCCTCTGACGTGTGCACCCAACGATATGATGATGGCGACGAAAGCGATGCATCGTCGTTA
CTGGTTTACTGATGTTCATGCTCGTGGATATTATCCGCAACATATGCTGAATTACTTTGCCAGGAAAGGATTCAACCTCG
ATATCACACCAGAAGATAACGCGATTCTTGCCAGAGGTTGTGTCGACTTTATCGGTTTTAGCTACTACATGTCTTTTACG
ACACAATTTTCGCCCGATAACCCGCAACTGGATTATGTTGAACCACGAGATTTGGTCAGCAACCCTTATATCGATACATC
CGAATGGGGATGGCAAATTGATCCGGCAGGGCTACGTTATTCACTCAACTGGTTGTGGGATCATTTCCAGTTGCCGCTGT
TTATTGTCGAAAATGGATTTGGTGCGGTTGACCAGAGACAAGCTGACGGCACAGTGAACGATCACTATCGCATTGATTAC
TTTGCTTCCCATATTCGTGAAATGAAAAAAGCCGTAGTCGAAGATGGTGTTGACTTAATTGGCTACACCCCGTGGGGCTG
CATTGACCTGGTTTCTGCCGGAACAGGGGAAATGAAAAAACGCTACGGAATGATTTATGTCGATAAAGACAACGAAGGGA
AGGGAACGCTGGAAAGGATACGTAAAGCGTCGTTTTACTGGTATCGGGATCTCATCGCCAACAATGGCGAAAATATTTGA

Upstream 100 bases:

>100_bases
ATTATCGAATTTTAAGTTTAATGAGAACAGCAAAGACGAGTTTATGGCCGGCATCCAGGCGGAAGTCTGGTGGTAATCTA
TTTTAGTTAAGGAGTTGTGT

Downstream 100 bases:

>100_bases
GACTTCAGAAAGATATTACGGAGATGTCAGGCTCTGTGATCAGAGCCTGACAATAACTACATAGCGTGTTGATTATAATA
GGGCACGCCGAGCGCATCCG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAVAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMG
FKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFT
TQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWLWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDY
FASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI

Sequences:

>Translated_479_residues
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAVAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMG
FKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFT
TQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWLWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDY
FASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
>Mature_478_residues
SGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAVAHGVPREVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGF
KCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVF
TRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCMI
AMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTT
QFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNWLWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYF
ASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=28.8577154308617, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI32481206, Length=493, Percent_Identity=27.3833671399594, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI13273313, Length=491, Percent_Identity=25.8655804480652, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI24497614, Length=488, Percent_Identity=30.1229508196721, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=27.4226804123711, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI2367174, Length=475, Percent_Identity=60.2105263157895, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1789070, Length=485, Percent_Identity=48.659793814433, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI2367270, Length=479, Percent_Identity=50.3131524008351, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17552856, Length=489, Percent_Identity=28.4253578732106, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17539390, Length=488, Percent_Identity=27.8688524590164, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21356577, Length=490, Percent_Identity=28.3673469387755, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55265; Mature: 55134

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAVAHGVPREVTEGVIDGLNYPNH
CCCCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
EAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEINNQ
CCCCEEEHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCC
VNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
CCCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDN
EEECCCCEEECCCCHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
AILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRY
CEEHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEE
SLNWLWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLI
EEEEHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEE
GYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
CCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAVAHGVPREVTEGVIDGLNYPNH
CCCCCCCEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
EAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEINNQ
CCCCEEEHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCC
VNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFNIGCM
CCCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDN
EEECCCCEEECCCCHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
AILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRY
CEEHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEECCCCCEE
SLNWLWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLI
EEEEHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEE
GYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
CCCCCCHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125345; 9384377 [H]