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The map label for this gene is ykgA [H]

Identifier: 157159805

GI number: 157159805

Start: 366188

End: 367045

Strand: Reverse

Name: ykgA [H]

Synonym: EcHS_A0352

Alternate gene names: 157159805

Gene position: 367045-366188 (Counterclockwise)

Preceding gene: 157159807

Following gene: 157159803

Centisome position: 7.9

GC content: 43.94

Gene sequence:

>858_bases
ATGATCAGGCAGAAGATTCTACAGCAGCTCCTGGAGTGGATTGAGTGCAATCTTGAGCACCCTATTTCAATCGAAGATAT
CGCACAGAAATCTGGCTACAGCAGACGCAACATCCAGCTTCTGTTCCGAAATTTCATGCATGTGCCTTTGGGAGAATACA
TTCGCAAACGAAGGCTTTGCCGTGCCGCTATTCTTGTCCGGCTCACCGCGAAATCTATGCTTGATATTGCACTCTCTTTG
CATTTTGATTCACAGCAGTCATTCAGCCGCGAATTTAAAAAGTTATTCGGCTGCTCTCCCCGTGAATACCGCCACCGTGA
TTATTGGGATCTCGCAAATATCTTCCCTTCTTTTTTAATACGTCAACAGCAAAAGACGGAGTGTAGATTAGTCAATTTTC
CTGAGACACCTATTTTTGGCAACTCATTTAAATATGACATTGAAGTGTCGAATAAATCACCGGATGAAGAAGTCAAACTG
CGACGTCATCATTTAGCCAGATGTATGAATAATTTTAAGACGGATATCTATTTCGTCTCCACGTTTGAACCGTCAACAAA
ATCGGTCGATTTGCTCACGGTTGAAACTTTTGCCGGTACGGTATGCAAACAAACCGATTCAGAGATGCCGAAAGAGTGGA
CAATTAACCGAGGACTGTATGCCTCTTTCCGCTATGAAGGTGAGTGGGAGCACTACCCTGAATGGGCGCGAAACCTCTAT
CTGATGGAATTACCCGCCAGGGGGTTAGCCAGAGTGAACGGCAGTGATATTGAGCGGTTTTATTACAATGAAGATTTCGT
AGAAAAGGATAGCAATGATGTTGTTTGCGAAATTTTTATCCCCGTTCGTCCGGTTTAG

Upstream 100 bases:

>100_bases
CTACCAGTGCTTTTGCCTTCGCCCTTGCTGTTTTTGATACACTTAGGGCCCAGGGCATAACGAAAATGTGCGATATGACA
AAGAATTAACGGAGAATGTG

Downstream 100 bases:

>100_bases
TTTTCGCTATATCTAAATGAATTTATAACGAGACTACTGTCAGGAAATTCAATAAATAATGGCGTTGAAGATGTCAACGC
CATTATTGTTTATTAGAATG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLY
LMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV

Sequences:

>Translated_285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLY
LMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
>Mature_285_residues
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSL
HFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLY
LMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=291, Percent_Identity=32.3024054982818, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1790497, Length=106, Percent_Identity=46.2264150943396, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081928, Length=98, Percent_Identity=36.734693877551, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33908; Mature: 33908

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLC
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLI
HHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
RQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKLRRHHLARCMNNFKTDIYFVS
HHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLY
ECCCCCCCEEEEEEHHHHHHHHHHCHHCCCCCEECCCCEEEEEEECCCCCCCHHHHHCEE
LMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
EEECCCCCHHHCCCCHHHHHCCCCHHHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRNFMHVPLGEYIRKRRLC
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPREYRHRDYWDLANIFPSFLI
HHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHH
RQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKLRRHHLARCMNNFKTDIYFVS
HHCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLY
ECCCCCCCEEEEEEHHHHHHHHHHCHHCCCCCEECCCCEEEEEEECCCCCCCHHHHHCEE
LMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVCEIFIPVRPV
EEECCCCCHHHCCCCHHHHHCCCCHHHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]