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The map label for this gene is potE [H]

Identifier: 157159107

GI number: 157159107

Start: 738364

End: 739683

Strand: Reverse

Name: potE [H]

Synonym: EcE24377A_0717

Alternate gene names: 157159107

Gene position: 739683-738364 (Counterclockwise)

Preceding gene: 157158479

Following gene: 157156449

Centisome position: 14.85

GC content: 50.45

Gene sequence:

>1320_bases
ATGAGTCAGGCTAAATCGAACAAAATGGGCGTCGTTCAGTTAACCATACTGACGATGGTCAACATGATGGGCTCCGGTAT
CATCATGCTGCCGACAAAGCTTGCCGAAGTTGGGACAATTTCAATTATCTCCTGGCTGGTGACAGCCGTAGGCTCAATGG
CACTGGCATGGGCATTCGCAAAATGCGGTATGTTCAGCCGTAAATCAGGTGGTATGGGCGGTTATGCCGAGTATGCTTTC
GGTAAGTCCGGCAATTTTATGGCGAACTACACCTACGGTGTCTCGCTGCTGATTGCTAACGTCGCGATTGCTATTTCGGC
GGTTGGTTACGGCACCGAACTGCTCGGCGCGAGTTTGTCGCCAGTGCAGATTGGTCTTGCGACCATCGGAGTGCTGTGGA
TTTGTACCGTGGCTAACTTTGGTGGTGCGCGCATTACCGGGCAAATCAGTAGCATTACTGTGTGGGGGGTCATTATTCCG
GTGGTAGGCCTGTGCATTATCGGCTGGTTCTGGTTTAGCCCGACGCTTTACGTTGATTCCTGGAATCCGCATCATGCACC
GTTCTTCAGTGCAGTAGGTTCTTCCATCGCTATGACGCTGTGGGCTTTTCTCGGTCTGGAGTCTGCGTGTGCGAATACTG
ATGTAGTGGAAAACCCGGAACGTAATATGCCAATCGCGGTACTCGGCGGTACGTTAGGTGCGGCGGTGATTTATATCGTC
TCCACCAACGTGATTGCAGGGATTGTGCCTAATATGGAGCTGGCAAATTCAACGGCACCATTTGGCCTGGCCTTCGCACA
GATGTTCACACCAGAAGTGGGTAAAGTCATTATGGCGCTGATGGTGATGTCCTGCTGCGGTTCGCTGCTTGGCTGGCAGT
TCACCATTGCCCAGGTGTTTAAATCTTCATCTGATGAAGGCTACTTCCCTAAAATTTTCTCCCGTGTAACCAAAGTGGAT
GCACCGGTGCAGGGAATGTTGACCATTGTGATTATCCAGAGCGGGTTGGCGCTGAAGACGATTAGCCCGTCGCTAAACAG
TCAGTTCAACGTGCTGGTTAACCTGGCCGTGGTGACCAATATCATTCCGTATATTCTGTCAATGGCGGCATTAGTCATTA
TTCAGAAGGTCGCTAATGTGCCCCCCTCAAAAGCGAAAGTTGCAAACTTTGTTGCTTTTGTTGGCGCGATGTATAGCTTT
TATGCGCTGTACTCATCCGGGGAAGAAGCCATGCTGTACGGTTCCATCGTGACCTTCCTTGGTTGGACACTGTATGGTCT
GGTATCACCACGCTTTGAACTGAAAAATAAACACGGTTAA

Upstream 100 bases:

>100_bases
AGGGTGTCTATATCGAAGAACATGATGGTCGTAAGCAAGTTTGGTGCTATGTCATCAAGCCTCGTGATGCGCAAAGCACC
CTGTTGAAAGGGGAAAAATT

Downstream 100 bases:

>100_bases
AAAAGCAAAGTAAAAAAAGGCGATCTTGCGACCGCCCTTTTTTTATTAAATGTGTTTACGCGTTTTTCAGAACTTCGCTA
ACAATCTCAACCGCTTCTTT

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNMPIAVLGGTLGAAVIYIV
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
APVQGMLTIVIIQSGLALKTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Sequences:

>Translated_439_residues
MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNMPIAVLGGTLGAAVIYIV
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
APVQGMLTIVIIQSGLALKTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG
>Mature_438_residues
SQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFG
KSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPV
VGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNMPIAVLGGTLGAAVIYIVS
TNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDA
PVQGMLTIVIIQSGLALKTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFY
ALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=439, Percent_Identity=99.5444191343963, Blast_Score=871, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=437, Percent_Identity=32.4942791762014, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1790575, Length=415, Percent_Identity=31.3253012048193, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787890, Length=422, Percent_Identity=27.2511848341232, Blast_Score=105, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17540018, Length=312, Percent_Identity=25.3205128205128, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46524; Mature: 46393

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLS
HCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYVDS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
WNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNMPIAVLGGTLGAAVIYIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQSGLALKTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCHHHHEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC
>Mature Secondary Structure 
SQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLS
HCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYVDS
HHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
WNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNMPIAVLGGTLGAAVIYIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQSGLALKTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCHHHHEEEEEEECCCEEEECCCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]