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The map label for this gene is speF [H]

Identifier: 157158479

GI number: 157158479

Start: 739680

End: 741887

Strand: Reverse

Name: speF [H]

Synonym: EcE24377A_0718

Alternate gene names: 157158479

Gene position: 741887-739680 (Counterclockwise)

Preceding gene: 157155566

Following gene: 157159107

Centisome position: 14.9

GC content: 48.37

Gene sequence:

>2208_bases
ATGAACAAAATGTCAGAATTAAAAATTGCCGTTAGTCGTTCTTGCCCGGATTTTTTTTCCACTCATCGTGCATGCGTGAA
TATAGACGAAAGTAATTATATTGACGTTGCCGCCATTATTTTATCAGTCAGTGATGTTGAACGTGGAAAACTCGATGAAA
TAGACGCTACTGGCTATGACATTCCTGTTTTTATTGCAACGGAAAATGAAGAACGTGTACCCGCAGAATATCTGCCACGT
ATTTCTGGTGTCTTCGAACATTGCGAATCACGTAAAGAGTTTTATGGACGTCAGTTAGAAACCGCTGCCAGCCATTATGA
AACGCAACTGCGCCCGCCCTTCTTCCGCGCACTGGTCGATTATGTTAATCAGGGCAATAGTGCTTTTGACTGCCCTGGTC
ATCAGGGGGGTGAGTTTTTCCGTCGCCATCCGGCAGGCAATCAGTTCGTGGAATACTTTGGCGAAATGCTCTTCCGCTCT
GACTTGTGTAATGCTGACGTAGCAATGGGCGATCTGCTGATTCATGAAGGTGCGCCATGTATTGCACAACAGCATGCGGC
TAAAGTCTTTAATGCCGATAAAACCTATTTCGTTTTAAACGGTACTTCATCTTCTAATAAAGTGGTCTTAAACGCATTGC
TGACGCCGGGCGATCTGGTGTTGTTTGACCGCAATAACCATAAATCTAACCACCACGGTGCGTTGCTACAGGCTGGCGCG
ACACCGGTTTATCTGGAAACCGCACGTAACCCGTATGGCTTTATCGGTGGCATTGATGCGCACTGTTTTGAAGAAAGTTA
CCTGCGTGAGCTGATTACAGAAGTTGCACCGCAGCGGGCAAAAGAGGCTCGTCCTTTCCGCCTCGCCGTTATTCAGCTAG
GCACTTACGACGGTACGATTTATAACGCCCGTCAGGTGGTGGATAAAATTGGTCATCTGTGTGACTACATCCTCTTTGAC
TCCGCATGGGTCGGCTATGAACAGTTTATTCCGATGATGGCTGACTGTTCGCCGCTGTTGCTGGATCTTAATGAGAACGA
TCCGGGTATTCTGGTTACGCAATCCGTACATAAACAGCAGGCTGGTTTTTCTCAAACTTCACAAATCCATAAAAAAGACA
GCCACATCAAAGGGCAACAGCGTTATGTACCGCACAAACGCATGAACAACGCCTTTATGATGCACGCCTCCACCAGCCCG
TTCTATCCGCTGTTTGCCGCACTGGATATCAACGCCAAAATGCATGAAGGTGTCAGCGGTCGTAATATGTGGATGGATTG
TGTGGTAAATGGCATTAATGCCCGCAAACTGATCCTCGATAACTGTCAGCATATTCGTCCGTTCGTACCTGAACTGGTGG
ATGGTAAACCCTGGCAGTCGTATGAAACGGCGCAAATTGCGGTTGATCTGCGCTTCTTCCAGTTTGTACCAGGGGAACAC
TGGCATTCTTTTGAAGGCTATGCAGAGAATCAATACTTTGTCGATCCTTGCAAACTGTTGCTGACAACCCCAGGTATTGA
TGCACGTAACGGCGAATATGAAGCGTTCGGTGTACCCGCGACGATTCTTGCTAACTTCCTGCGCGAAAATGGCGTAGTGC
CGGAAAAATGCGATCTTAACTCCATCCTCTTCCTGCTGACTCCGGCAGAAGATATGGCCAAGCTTCAGCAACTTGTTGCC
CTGCTGGTACGCTTCGAAAAACTGCTTGAGTCCGACGCGCCATTAGCAGAAGTGCTGCCTTCCATCTACAAACAGCATGA
AGAGCGCTACGCCGGTTATACCCTGCGTCAGTTGTGTCAAGAAATGCATGATTTGTATGCCCGTCACAACGTGAAACAAC
TGCAAAAAGAGATGTTCCGTAAGGAACACTTCCCACGCGTAAGCATGAATCCGCAAGAAGCCAACTACGCCTATTTACGC
GGTGAAGTGGAGCTGGTTCGTCTGCCGGATGCAGAAGGCCGTATCGCTGCCGAAGGTGCGCTTCCTTATCCTCCGGGTGT
GCTGTGTGTTGTTCCGGGTGAAATCTGGGGTGGTGCTGTTTTGCGTTACTTCAGCGCTCTGGAAGAAGGGATCAACCTGC
TGCCAGGTTTTGCACCGGAGCTGCAGGGTGTCTATATCGAAGAACATGATGGTCGTAAGCAAGTTTGGTGCTATGTCATC
AAGCCTCGTGATGCGCAAAGCACCCTGTTGAAAGGGGAAAAATTATGA

Upstream 100 bases:

>100_bases
CTACTGATTTATTTATCAGCGGTGGAGCTTTGCTTTTTTTCCGGCGTGATCGATTTCTCCTTTGAGAAATTGAGGACCTG
CTATTACCTGAAATAAAGAG

Downstream 100 bases:

>100_bases
GTCAGGCTAAATCGAACAAAATGGGCGTCGTTCAGTTAACCATACTGACGATGGTCAACATGATGGGCTCCGGTATCATC
ATGCTGCCGACAAAGCTTGC

Product: ornithine decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MNKMSELKIAVSRSCPDFFSTHRACVNIDESNYIDVAAIILSVSDVERGKLDEIDATGYDIPVFIATENEERVPAEYLPR
ISGVFEHCESRKEFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRS
DLCNADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGA
TPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFD
SAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSP
FYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEH
WHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVA
LLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLR
GEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVI
KPRDAQSTLLKGEKL

Sequences:

>Translated_735_residues
MNKMSELKIAVSRSCPDFFSTHRACVNIDESNYIDVAAIILSVSDVERGKLDEIDATGYDIPVFIATENEERVPAEYLPR
ISGVFEHCESRKEFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRS
DLCNADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGA
TPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFD
SAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSP
FYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEH
WHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVA
LLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLR
GEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVI
KPRDAQSTLLKGEKL
>Mature_735_residues
MNKMSELKIAVSRSCPDFFSTHRACVNIDESNYIDVAAIILSVSDVERGKLDEIDATGYDIPVFIATENEERVPAEYLPR
ISGVFEHCESRKEFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRS
DLCNADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGA
TPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFD
SAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSP
FYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEH
WHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVA
LLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLR
GEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVI
KPRDAQSTLLKGEKL

Specific function: Spermidine biosynthesis from ornithine. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786909, Length=732, Percent_Identity=96.7213114754098, Blast_Score=1478, Evalue=0.0,
Organism=Escherichia coli, GI87082193, Length=719, Percent_Identity=67.1766342141864, Blast_Score=1020, Evalue=0.0,
Organism=Escherichia coli, GI221142684, Length=679, Percent_Identity=31.8114874815906, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1790573, Length=636, Percent_Identity=33.4905660377358, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1786384, Length=659, Percent_Identity=32.1699544764795, Blast_Score=301, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR005308
- InterPro:   IPR011193
- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C; PF03709 OKR_DC_1_N [H]

EC number: =4.1.1.17 [H]

Molecular weight: Translated: 82973; Mature: 82973

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00703 OKR_DC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMSELKIAVSRSCPDFFSTHRACVNIDESNYIDVAAIILSVSDVERGKLDEIDATGYD
CCCHHHHHHHHHCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRPPFFRALVD
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLCNADVAMGDLLIHEGAPC
HHHCCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCH
IAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGA
HHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCC
TPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTI
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEEEEECCCCCC
YNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHH
AGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSG
CCCHHHHHHHHHHHCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCC
RNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEH
CCHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCC
WHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLN
CCCCCCCCCCCEEECCHHEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCC
SILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQ
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGA
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEECCC
LPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVI
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEEE
KPRDAQSTLLKGEKL
CCCCHHHHHHCCCCC
>Mature Secondary Structure
MNKMSELKIAVSRSCPDFFSTHRACVNIDESNYIDVAAIILSVSDVERGKLDEIDATGYD
CCCHHHHHHHHHCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRPPFFRALVD
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLCNADVAMGDLLIHEGAPC
HHHCCCCEECCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCH
IAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGA
HHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCEEECCC
TPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTI
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEEEEEEECCCCCC
YNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHH
AGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSG
CCCHHHHHHHHHHHCCCCCHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHCCCCC
RNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEH
CCHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHCCCCCCC
WHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLN
CCCCCCCCCCCEEECCHHEEEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCC
SILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQ
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
EMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGA
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEECCC
LPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVI
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEEE
KPRDAQSTLLKGEKL
CCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939141; 8905232; 9278503 [H]