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The map label for this gene is helD [C]

Identifier: 157158381

GI number: 157158381

Start: 3376149

End: 3377678

Strand: Reverse

Name: helD [C]

Synonym: EcE24377A_3358

Alternate gene names: 157158381

Gene position: 3377678-3376149 (Counterclockwise)

Preceding gene: 157157710

Following gene: 157156708

Centisome position: 67.83

GC content: 56.34

Gene sequence:

>1530_bases
ATGTCACTGACACCCACCGATGAACAGTCTGCCATTATTCACTGGCAGGGACAGAAGCTGGTTGTGAACGCCTTTGCCGG
CACAGGAAAGACCAGTACCCTGGTTCAGTATGCGCTGGCAAACCCCGACAGCAGAATACTGTATCTGGCTTATAACCGTT
CTGTACGCGATGAGGCTGAGCGTAAATTTCCGTTTAATGTGGAGTGCAAAACCTCCCACCAACTGGCCTGGTCGCGCTTC
GGCAGACATTACCAGAATCGCCTGGTTGCCAGTCTGCGTATCACGGATGTGGCCAGAAAGCTGAACACCCGTCACTGGCT
GCTGGTTCGTCTTGCACTCAGCAGTCTGAATCAGTTTCTGTGCAGTGCTGACAAGGAGCCGGGGCCGCAGCATTTGCCGG
ATGAGGACGCCCGGGCCGGCACGGATGTTTCAGCCATTCTGCAGGCAGTACAGGTGCTATGGCATGAAATGAGTCGCCCT
GATGGCACATTCCCTGTCACGCATGATACCTACCTGAAGCTGTTCCAGTTGTCATCCCCGGACCTGTCCCGGCGCTGGGA
CATCATTCTGTTTGATGAGGCACAGGACGCCAATCCGGTGACGTCTGCACTGGTGCTCAGTCAGCCCTGCAGGGTAATCC
TGGTGGGCGACCGTTACCAGCAGATTTACCGCTTTCGTGGTGCAGATAATGCGCTTTCTCATCCTGTTCTGAACGATGCA
GGCCGTCTGTGGCTGACACAGAGCTTCCGCTTTGGCCCGTCAGTGGCCCGGATGGCGAATCTGCTTTTGCAGCGTGCCGG
TGAGAAACGGCAGGTGACCGGCAGTGGCGGGGAAGATGACGTACTGATGAGCCGTACCGACGCGGATAAACCGGAAGGGC
ACCGGACGGTACTGAGCCGGACGGTACTGAGCCGGACGGTGGCAGGGGTGATATGCACGGCGCTGATGGCCAGTCTGAGC
GGCAGGAAGGTGTACTGGGTGGGGGGTATTGAAGGCTACCGGACTGAGGCACTGGAGGACCTGTACTGGTTTTCAGCTGA
TATGCCGGAAAAGATGCAGTCTGATGCACTGCGGCGGGATTACCGCGATTTTGATGAATACTGCCGGATAGCCAAATCCA
CCCGGGATGTGGAGATGAATCAGGCCATTCGCCTGCTGGATATCTGTTTCCCGTTACCGGTGAAACTGAAGCTTCTGCGG
GAGCATACCGTCACGGGTGAGAAGGATGCGGATATTACCGTGTCAACGGCTCACCGCAGTAAGGGACTGGAATGGCCGGT
GGTGATACTGGATGAGGATTTTGCGGATATCACCGACCCGCTGATGCCGGAAGATGAACGGAGGGACGAAACTAACCTGT
TGTACGTTGCGGTCACCCGGGCCCGTAAAACCCTGGTGCTGAACAGTCTGATGCGCCAGCTGGCAGAAGAAGCTGGCAGA
GATGAACAAAACCAGAAAGCAGCAGAGGCATCCCCGTCAGAAGAAGGGGAAAACGATGCCGTATACGGGCAGGAGGATGA
AAATGCTTAA

Upstream 100 bases:

>100_bases
GGACCGAATAATTTCCCGACACCGGTAAAGAGTTAATGATGGTGTGCACGTTCTGCAGTGATGCAGAGTGTGTGCACTAT
TTTTCTTTACAGGGGCCTGT

Downstream 100 bases:

>100_bases
TCGCCTGAAAAAACTGCTTCCCGGTAACAGCAATACCAGTAGTGCTGAGACAACCGCCCCGGAAACCGCACGCCAGCCGG
AACATCTGCCGGAAGGTTTT

Product: hypothetical protein

Products: Separated DNA [C]

Alternate protein names: Superfamily I DNA/RNA Helicase-Like Protein; Helicase; Superfamily I DNA/RNA Helicase; Superfamily I DNA And RNA Helicase; Helicase Superfamily Protein I; UvrD/REP Helicase; F-Box Only Protein; I DNA And RNA Helicase Protein; UvrD/Rep Family Helicase; I DNA And RNA Helicase; I DNA/RNA Helicase; Helicase Superfamily I

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSLTPTDEQSAIIHWQGQKLVVNAFAGTGKTSTLVQYALANPDSRILYLAYNRSVRDEAERKFPFNVECKTSHQLAWSRF
GRHYQNRLVASLRITDVARKLNTRHWLLVRLALSSLNQFLCSADKEPGPQHLPDEDARAGTDVSAILQAVQVLWHEMSRP
DGTFPVTHDTYLKLFQLSSPDLSRRWDIILFDEAQDANPVTSALVLSQPCRVILVGDRYQQIYRFRGADNALSHPVLNDA
GRLWLTQSFRFGPSVARMANLLLQRAGEKRQVTGSGGEDDVLMSRTDADKPEGHRTVLSRTVLSRTVAGVICTALMASLS
GRKVYWVGGIEGYRTEALEDLYWFSADMPEKMQSDALRRDYRDFDEYCRIAKSTRDVEMNQAIRLLDICFPLPVKLKLLR
EHTVTGEKDADITVSTAHRSKGLEWPVVILDEDFADITDPLMPEDERRDETNLLYVAVTRARKTLVLNSLMRQLAEEAGR
DEQNQKAAEASPSEEGENDAVYGQEDENA

Sequences:

>Translated_509_residues
MSLTPTDEQSAIIHWQGQKLVVNAFAGTGKTSTLVQYALANPDSRILYLAYNRSVRDEAERKFPFNVECKTSHQLAWSRF
GRHYQNRLVASLRITDVARKLNTRHWLLVRLALSSLNQFLCSADKEPGPQHLPDEDARAGTDVSAILQAVQVLWHEMSRP
DGTFPVTHDTYLKLFQLSSPDLSRRWDIILFDEAQDANPVTSALVLSQPCRVILVGDRYQQIYRFRGADNALSHPVLNDA
GRLWLTQSFRFGPSVARMANLLLQRAGEKRQVTGSGGEDDVLMSRTDADKPEGHRTVLSRTVLSRTVAGVICTALMASLS
GRKVYWVGGIEGYRTEALEDLYWFSADMPEKMQSDALRRDYRDFDEYCRIAKSTRDVEMNQAIRLLDICFPLPVKLKLLR
EHTVTGEKDADITVSTAHRSKGLEWPVVILDEDFADITDPLMPEDERRDETNLLYVAVTRARKTLVLNSLMRQLAEEAGR
DEQNQKAAEASPSEEGENDAVYGQEDENA
>Mature_508_residues
SLTPTDEQSAIIHWQGQKLVVNAFAGTGKTSTLVQYALANPDSRILYLAYNRSVRDEAERKFPFNVECKTSHQLAWSRFG
RHYQNRLVASLRITDVARKLNTRHWLLVRLALSSLNQFLCSADKEPGPQHLPDEDARAGTDVSAILQAVQVLWHEMSRPD
GTFPVTHDTYLKLFQLSSPDLSRRWDIILFDEAQDANPVTSALVLSQPCRVILVGDRYQQIYRFRGADNALSHPVLNDAG
RLWLTQSFRFGPSVARMANLLLQRAGEKRQVTGSGGEDDVLMSRTDADKPEGHRTVLSRTVLSRTVAGVICTALMASLSG
RKVYWVGGIEGYRTEALEDLYWFSADMPEKMQSDALRRDYRDFDEYCRIAKSTRDVEMNQAIRLLDICFPLPVKLKLLRE
HTVTGEKDADITVSTAHRSKGLEWPVVILDEDFADITDPLMPEDERRDETNLLYVAVTRARKTLVLNSLMRQLAEEAGRD
EQNQKAAEASPSEEGENDAVYGQEDENA

Specific function: Helicase IV Catalyzes The Unwinding Of Duplex DNA In The 3' To 5' Direction With Respect To The Bound Single Strand In A Reaction That Is Dependent Upon The Hydrolysis Of ATP. [C]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI30795119, Length=514, Percent_Identity=32.1011673151751, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI30795117, Length=514, Percent_Identity=32.1011673151751, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.6.1.- [C]

Molecular weight: Translated: 57500; Mature: 57369

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTPTDEQSAIIHWQGQKLVVNAFAGTGKTSTLVQYALANPDSRILYLAYNRSVRDEAE
CCCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHH
RKFPFNVECKTSHQLAWSRFGRHYQNRLVASLRITDVARKLNTRHWLLVRLALSSLNQFL
HCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
CSADKEPGPQHLPDEDARAGTDVSAILQAVQVLWHEMSRPDGTFPVTHDTYLKLFQLSSP
HCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCC
DLSRRWDIILFDEAQDANPVTSALVLSQPCRVILVGDRYQQIYRFRGADNALSHPVLNDA
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCHHCCCCCCCC
GRLWLTQSFRFGPSVARMANLLLQRAGEKRQVTGSGGEDDVLMSRTDADKPEGHRTVLSR
CCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHH
TVLSRTVAGVICTALMASLSGRKVYWVGGIEGYRTEALEDLYWFSADMPEKMQSDALRRD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YRDFDEYCRIAKSTRDVEMNQAIRLLDICFPLPVKLKLLREHTVTGEKDADITVSTAHRS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHC
KGLEWPVVILDEDFADITDPLMPEDERRDETNLLYVAVTRARKTLVLNSLMRQLAEEAGR
CCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
DEQNQKAAEASPSEEGENDAVYGQEDENA
CHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLTPTDEQSAIIHWQGQKLVVNAFAGTGKTSTLVQYALANPDSRILYLAYNRSVRDEAE
CCCCCCCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHH
RKFPFNVECKTSHQLAWSRFGRHYQNRLVASLRITDVARKLNTRHWLLVRLALSSLNQFL
HCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
CSADKEPGPQHLPDEDARAGTDVSAILQAVQVLWHEMSRPDGTFPVTHDTYLKLFQLSSP
HCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCC
DLSRRWDIILFDEAQDANPVTSALVLSQPCRVILVGDRYQQIYRFRGADNALSHPVLNDA
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCHHCCCCCCCC
GRLWLTQSFRFGPSVARMANLLLQRAGEKRQVTGSGGEDDVLMSRTDADKPEGHRTVLSR
CCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHH
TVLSRTVAGVICTALMASLSGRKVYWVGGIEGYRTEALEDLYWFSADMPEKMQSDALRRD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YRDFDEYCRIAKSTRDVEMNQAIRLLDICFPLPVKLKLLREHTVTGEKDADITVSTAHRS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECHHC
KGLEWPVVILDEDFADITDPLMPEDERRDETNLLYVAVTRARKTLVLNSLMRQLAEEAGR
CCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
DEQNQKAAEASPSEEGENDAVYGQEDENA
CHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Duplex DNA; DNA [C]

Specific reaction: Catalyzes the unwinding and separation of duplex DNA in vivo Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA