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The map label for this gene is ycaN [H]

Identifier: 157157918

GI number: 157157918

Start: 998185

End: 999093

Strand: Reverse

Name: ycaN [H]

Synonym: EcE24377A_0977

Alternate gene names: 157157918

Gene position: 999093-998185 (Counterclockwise)

Preceding gene: 157154815

Following gene: 157157127

Centisome position: 20.06

GC content: 46.42

Gene sequence:

>909_bases
ATGCGGATGAATATGTCTGACTTTGCCACTTTCTTTGCCGTGGCCCGTAATCAAAGCTTTCGTGCAGCGGGCGATGAATT
AGGCTTATCCTCGTCCGCCATTAGCCATAGTATTAAAACACTGGAACAACGTCTTAAAATTCGCTTATTCAATCGGACAA
CCCGAAGCGTATCGTTAACGGAAGCGGGTTCTAATCTCTATGAACGATTGCGCCCAGCTTTCGACGAAATTCAGATCATG
TTAGATGAGATGAATGATTTTCGTCTGACACCGACAGGAACGCTCAAAATCAATGCCGCCCGGGTAGCTGCACGTATATT
TTTAATGCCTTTACTGGTTGGTTTCACACGTGAATATCCTGATATTAAAGTCGAGCTCACAACTGACGATTCTCTTGTCG
ATATCGTCCAACAGGGCTTTGACGCTGGCGTTCGACTTAGTGGCATCGTCGAAAAAGATATGATCAGCGTGGCGATCGGC
CCACCAGTGAAATTATGTGTAGCAGCAACGCCAGAGTACTTTGCAAGATATGGCAAACCCCGCCATCCTCATGATCTGCT
TAATCATCAATGTGTTGTTTTTCGTTATCCCAGCGGCAAACCTTTCCACTGGCAATTTGCAAAGGAGCTGGAAATTGCTG
TCGCAGGAAATATCATTCTGGATGATGTTGATGCTGAACTGGAGGCCGTTTTGATGGGTGCTGGTATCGGGTATCTGCTC
TATGAACAGATCAAGGAGTACCTTGATACTGGTCGTTTAGAATGTGTACTGGAAGACTGGAGCACAGAGCGTTCAGGCTT
CCAGATCTATTATCCAAACCGCCAGTATATGTCCTGCGGCTTGCGCGCATTTCTCGATTATGTAAAAACGGGTCAGATCT
GCCAGAGTCAGCGTCACCGACCACAATAA

Upstream 100 bases:

>100_bases
TAATTTTACCTCCACGTTAAGTTCTGGAGCGCACTTTACGCAGGCGGCATAATCCTGTAAACGTGATGATTCAGGATGAG
TTAATGAAGGATTTTCACTA

Downstream 100 bases:

>100_bases
TTATTTACTATTCGCTTTACGCGCCAGCAGCGGGAATATCAGTCCTAATCCTACCAGCACAAACGGCGTTGCAACATTCA
GCGCCAACTGGAAGGTCCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Sequences:

>Translated_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ
>Mature_302_residues
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIM
LDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
YEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=99.0066225165563, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=33.5616438356164, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=28.7162162162162, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.3737024221453, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789639, Length=304, Percent_Identity=25.9868421052632, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=28.7234042553192, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=24.496644295302, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=27.4576271186441, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=29.2929292929293, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=34.9593495934959, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=23.2786885245902, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=29.1925465838509, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=140, Percent_Identity=25.7142857142857, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=31.8965517241379, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34249; Mature: 34249

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEECCHHHHHEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCHHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC
>Mature Secondary Structure
MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQRLKIRLFNRTTRSVSLT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
EAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARVAARIFLMPLLVGFTREYP
HHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
DIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKP
CEEEEEECCHHHHHHHHHCCCCCCEEECCHHHHHEEEECCCCEEEEEECCHHHHHHCCCC
RHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLL
CCCHHHHCCCEEEEECCCCCCEEEEHHHHEEEEEECCEEEECCCHHHHHHHHHCCHHHHH
YEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHR
HHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCC
PQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]