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The map label for this gene is pflA [H]

Identifier: 157154815

GI number: 157154815

Start: 999865

End: 1000662

Strand: Reverse

Name: pflA [H]

Synonym: EcE24377A_0980

Alternate gene names: 157154815

Gene position: 1000662-999865 (Counterclockwise)

Preceding gene: 157155436

Following gene: 157157918

Centisome position: 20.1

GC content: 49.37

Gene sequence:

>798_bases
ATGAGTAATTTAACTAATTGTAATACAACAGAAAATACCGCTACGGCGGGGGCTACCAAGTCAGTTATTGGTCGCATTCA
CTCCTTTGAATCCTGTGGAACCGTAGACGGCCCAGGTATTCGCTTTATCACCTTTTTCCAGGGCTGCCTGATGCGCTGCC
TGTATTGTCATAACCGCGACACCTGGGATACGCATGGCGGTAAAGAAGTTACCGTTGAAGATTTAATGAAAGAGGTGGTG
ACCTATCGCCACTTTATGAACGCTTCCGGCGGCGGCGTTACCGCATCCGGCGGTGAGGCAATCCTACAAGCTGAGTTTGT
TCGTGACTGGTTCCGCGCCTGCAAAAAAGAAGGCATTCATACCTGTCTGGACACCAACGGTTTTGTTCGTCGTTACGATC
CGGTGATTGATGAACTGCTGGAAGTAACCGACCTGGTAATGCTCGATCTCAAACAGATGAACGACGAGATCCACCAAAAT
CTGGTTGGAGTTTCCAATCACCGCACGCTGGAGTTCGCCAAATATCTGGCGAACAAAAATGTGAAGGTGTGGATCCGCTA
CGTTGTTGTCCCAGGCTGGTCTGACGATGACGATTCAGCGCATCGCCTCGGTGAATTTACCCGTGATATGGGCAACGTTG
AGAAAATCGAGCTTCTCCCCTACCACGAGCTGGGCAAACACAAATGGGTGGCAATGGGTGAAGAGTACAAACTCGACGGT
GTTAAACCACCGAAGAAAGAGACCATGGAACGCGTGAAAGGCATTCTTGAGCAGTACGGTCATAAGGTAATGTTCTAA

Upstream 100 bases:

>100_bases
GCACCGGATTTCTTACAAGATGCTGTGACTCTTAACCCGGTGTCAGGAATGTCCATAATGCGTCCATAAACCAGAGTTAA
CTAGAGCCAATTGAAGCGAT

Downstream 100 bases:

>100_bases
TTTCCCCGTAAAGCGGCAACTTTATTGAGTTGCCGCTTTTTTATTCCGCGCTTCATTCTGCTACAGCGTTTTTAACACAT
CTTATAGTGCATCTACCATA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQN
LVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
VKPPKKETMERVKGILEQYGHKVMF

Sequences:

>Translated_265_residues
MSNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVV
TYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQN
LVGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
VKPPKKETMERVKGILEQYGHKVMF
>Mature_264_residues
SNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEDLMKEVVT
YRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHTCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNL
VGVSNHRTLEFAKYLANKNVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGV
KPPKKETMERVKGILEQYGHKVMF

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=245, Percent_Identity=100, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1790389, Length=291, Percent_Identity=25.4295532646048, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=27.1777003484321, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI226510931, Length=171, Percent_Identity=32.7485380116959, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30099; Mature: 29967

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIH
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCH
TCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
VKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCC
VKPPKKETMERVKGILEQYGHKVMF
CCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNLTNCNTTENTATAGATKSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TWDTHGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIH
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCH
TCLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
VKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDG
EEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCEECCC
VKPPKKETMERVKGILEQYGHKVMF
CCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]