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The map label for this gene is phnF

Identifier: 157156155

GI number: 157156155

Start: 4653583

End: 4654308

Strand: Reverse

Name: phnF

Synonym: EcE24377A_4656

Alternate gene names: 157156155

Gene position: 4654308-4653583 (Counterclockwise)

Preceding gene: 157157797

Following gene: 157156248

Centisome position: 93.47

GC content: 61.71

Gene sequence:

>726_bases
ATGCACTTGTCTACACATCCGACCAGCTATCCAACACGCTATCAGGAGATAGCCGCAAAACTTGAGCAGGAGCTTCGTCA
ACACTACCGCTGCGGCGACTATCTTCCCGCCGAGCAGCAACTGGCAGCGCGCTTTGAGGTGAATCGCCACACCCTGCGCC
GCGCCATCGACCAACTGGTGGAAAAAGGCTGGGTACAGCGCCGTCAGGGCGTCGGCGTGCTGGTGCTGATGCGCCCGTTC
GATTACCCGCTCAACGCCCAGGCGCGTTTTAGCCAGAATCTGCTGGATCAGGGCAGCCATCCCACCAGCGAAAAACTGCT
TTCGGTATTGCGCCCCGCGTCCGGCCACGTCGCTGACGCACTGGGGATTACCGAGGGGGAGAACGTCATCCACCTGCGCA
CCCTGCGTCGGGTCAACGGCGTCGCGCTCTGTTTAATCGACCACTACTTCGCGGACCTCACCCTCTGGCCGACGCTGCAA
CGCTTCGACAGCGGCTCGCTGCACGATTTTCTGCGCGAGCAAACCGGAATTGCGCTGCGCCGCAGCCAGACGCGGATCAG
CGCCCGTCGCGCCCAGGCCAAAGAGTGCCAGCGTCTTGAAATTCCGAATATGTCGCCGCTGCTGTGCGTGCGCACCCTTA
ACCACCGTGACGGTGAAAGCAGCCCGGCGGAGTACTCCGTCAGCCTGACGCGCGCCGACATGATTGAATTCACTATGGAG
CACTGA

Upstream 100 bases:

>100_bases
GCGCCCTGATGGTGCTGATTATCGTCACGGTCAGCCTGCTGGATTTCCTCTCTCAACGGTTGCGTAAGCACTTTATCTGA
TAAGCGAGGCATTGATATCT

Downstream 100 bases:

>100_bases
ATGCACGCAGATACCGCGACCCGCCAGCACTGGATGTCCGTACTGGCGCACAGCCAACCGGCTGAACTGGCAGCCCGCCT
GAAAGCACTGAACATCACCG

Product: phosphonate metabolism transcriptional regulator PhnF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H

Sequences:

>Translated_241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H
>Mature_241_residues
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPF
DYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
H

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known. By similarity could be a transcriptional regulator

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790540, Length=241, Percent_Identity=100, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1788418, Length=214, Percent_Identity=28.0373831775701, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790118, Length=231, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786950, Length=233, Percent_Identity=27.8969957081545, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHNF_ECOLI (P16684)

Other databases:

- EMBL:   J05260
- EMBL:   D90227
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G35718
- RefSeq:   AP_004604.1
- RefSeq:   NP_418526.1
- PDB:   2FA1
- PDBsum:   2FA1
- ProteinModelPortal:   P16684
- SMR:   P16684
- STRING:   P16684
- EnsemblBacteria:   EBESCT00000002305
- EnsemblBacteria:   EBESCT00000015676
- GeneID:   948617
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4063
- KEGG:   eco:b4102
- EchoBASE:   EB0709
- EcoGene:   EG10715
- eggNOG:   COG2188
- GeneTree:   EBGT00050000008760
- HOGENOM:   HBG696565
- OMA:   ASRFAVN
- ProtClustDB:   PRK10079
- BioCyc:   EcoCyc:EG10715-MONOMER
- Genevestigator:   P16684
- GO:   GO:0005622
- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00035
- SMART:   SM00345
- SMART:   SM00866
- TIGRFAMs:   TIGR02325

Pfam domain/function: PF00392 GntR; PF07702 UTRA

EC number: NA

Molecular weight: Translated: 27622; Mature: 27622

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
LGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQRFDSGSLHDFLREQTGIALR
HCCCCCCCEEEHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
RSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCC
H
C
>Mature Secondary Structure
MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
LGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQRFDSGSLHDFLREQTGIALR
HCCCCCCCEEEHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHH
RSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503