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The map label for this gene is lsrK

Identifier: 157154927

GI number: 157154927

Start: 1714791

End: 1716383

Strand: Reverse

Name: lsrK

Synonym: EcE24377A_1712

Alternate gene names: 157154927

Gene position: 1716383-1714791 (Counterclockwise)

Preceding gene: 157159022

Following gene: 157155287

Centisome position: 34.47

GC content: 52.29

Gene sequence:

>1593_bases
ATGGCTCGACTCTTTACCCCTTCAGAATCAAAGTATTACCTGATGGCGCTGGATGCAGGTACCGGAAGTATTCGGGCTGT
GATATTCGACCTGGAAGGCAATCAAATAGCAGTGGGACAGGCGGAGTGGCGGCACCTGGCAGTACCGGACGTTCCTGGTT
CTATGGAATTTGATCTCAACAAAAACTGGCAACTGGCGTGTGAGTGTATGCGCCAGGCGCTGCACAACGCCGGCATAGCC
CCGGAGTATATCGCTGCCGTTTCGGCATGTTCGATGCGTGAAGGCATTGTTTTATATAATAATGAAGGAGCCCCGATCTG
GGCCTGCGCCAATGTGGATGCCAGAGCGGCACGCGAAGTTAGCGAACTTAAAGAACTGCACAACAATACCTTTGAAAACG
AAGTTTATCGCGCGACCGGACAAACACTGGCTTTAAGTGCCATCCCCAGATTACTTTGGCTGGCGCACCATCGTTCCGAT
ATTTACCGTCAGGCATCAACCATCACCATGATCAGCGACTGGCTGGCCTATATGCTCAGCGGCGAACTGGCGGTGGATCC
CTCTAACGCTGGCACCACGGGACTTCTTGATCTAACCACCCGTGACTGGAAACCTGCATTGCTGGATATGGCTGGCCTGC
GTGCCGATATTCTTTCTCCTGTCAAAGAAACCGGCACATTGCTGGGCGTGGTAAGTTCACAAGCGGCGGAACTCTGCGGT
CTGAAGGCGGGCACTCCGGTGGTCGTTGGAGGAGGCGACGTGCAGCTTGGTTGCCTTGGGTTAGGCGTTGTGCGTCCGGC
ACAAACCGCGGTTCTTGGCGGCACATTCTGGCAGCAAGTTGTAAATTTAGCCGCGCCGGTGACAGACCCAGAAATGAACG
TGCGCGTTAATCCTCATGTTATTCCTGGCATGGTACAAGCTGAATCTATAAGCTTTTTTACCGGACTCACCATGCGCTGG
TTCCGCGATGCTTTCTGTGCCGAAGAAAAACTGATTGCCGAACGTTTAGGCATCGACACCTATACGCTGCTGGAAGAGAT
GGCCAGTCGGGTGCCGCCTGGGTCGTGGGGCGTAATGCCGATCTTCTCCGACAGAATGCGCTTTAAAACCTGGTATCACG
CTGCGCCTTCCTTTATTAACTTGTCCATTGACCCGGATAAATGTAACAAAGCGACATTGTTCCGTGCGCTGGAAGAAAAT
GCGGCGATTGTATCAGCGTGTAACTTGCAGCAAATTGCTGATTTCTCGAATATTCATCCTTCATCGTTAGTCTTTGCAGG
CGGAGGTTCAAAAGGGAAATTATGGAGTCAAATTCTCGCTGATGTCTCGGGATTACCCGTCAATATTCCGGTGGTCAAAG
AAGCCACTGCATTAGGATGTGCCATTGCAGCTGGCGTCGGTGCCGGAATTTTTTCATCAATGGCAGAAACCGGAGAACGC
CTGGTTCGCTGGGAACGGACGCACACACCAGACCCGGAAAAGCATGAACTTTATCAGGATTCACGCGATAAGTGGCAGGC
AGTTTATCAGGATCAGCTGGGGCTGGTTGATCATGGACTGACGACGTCGTTATGGAAAGCGCCTGGGTTATAG

Upstream 100 bases:

>100_bases
AGCGGCGATTTTACGTAGTTAAATTTGCATGACCTTATTACCGGGAATGATGGAGAACGTATAGAGGAACAGACAGAACG
CATAAGCCGAGGATAATCTA

Downstream 100 bases:

>100_bases
TTTCATTGACAGCCACATCATGATGACCGTGGCTGTTAGTTTTTGAGTATAGTTATTCTTATTTCCCTTCTTTTAGAGCT
GGCTTGTTAAAAGCCAGCTT

Product: autoinducer-2 (AI-2) kinase

Products: NA

Alternate protein names: AI-2 kinase

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIA
PEYIAAVSACSMREGIVLYNNEGAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Sequences:

>Translated_530_residues
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIA
PEYIAAVSACSMREGIVLYNNEGAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRW
FRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEEN
AAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
>Mature_529_residues
ARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAP
EYIAAVSACSMREGIVLYNNEGAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDI
YRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCGL
KAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWF
RDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENA
AIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGERL
VRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL

Specific function: Catalyzes the phosphorylation of autoinducer 2 (AI-2) to phospho-AI-2, which subsequently inactivates the transcriptional regulator LsrR and leads to the transcription of the lsr operon. Phosphorylates the ring-open form of 4,5-dihydroxy-2,3- pentanedione

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Escherichia coli, GI1787789, Length=530, Percent_Identity=99.811320754717, Blast_Score=1090, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=505, Percent_Identity=25.3465346534653, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=26.8924302788845, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789138, Length=508, Percent_Identity=24.0157480314961, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789168, Length=505, Percent_Identity=23.3663366336634, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789987, Length=510, Percent_Identity=25.4901960784314, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17535599, Length=274, Percent_Identity=26.2773722627737, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSRK_ECO24 (A7ZLW9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462798.1
- ProteinModelPortal:   A7ZLW9
- SMR:   A7ZLW9
- STRING:   A7ZLW9
- EnsemblBacteria:   EBESCT00000018833
- GeneID:   5586283
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1712
- NMPDR:   fig|331111.3.peg.4153
- eggNOG:   COG1070
- GeneTree:   EBGT00050000009228
- HOGENOM:   HBG337781
- OMA:   PIFSDAM
- ProtClustDB:   PRK10939
- BioCyc:   ECOL331111:ECE24377A_1712-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018484
- PANTHER:   PTHR10196

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: NA

Molecular weight: Translated: 57529; Mature: 57398

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNEGAPIWACANVDARAAREV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
IFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
HHHCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCC
SSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
CCEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
ARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFDLN
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCEEEECCCCCCEEECCCCCCCEEECCC
KNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNEGAPIWACANVDARAAREV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCHHHHHHH
SELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQASTITMISDWLAYMLS
HHHHHHHCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADILSPVKETGTLLGVVSSQAAELCG
CCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHC
LKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTFWQQVVNLAAPVTDPEMNVRVNPHV
CCCCCEEEECCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCC
IPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAERLGIDTYTLLEEMASRVPPGSWGVMP
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCH
IFSDRMRFKTWYHAAPSFINLSIDPDKCNKATLFRALEENAAIVSACNLQQIADFSNIHP
HHHCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCC
SSLVFAGGGSKGKLWSQILADVSGLPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGER
CCEEEECCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRWERTHTPDPEKHELYQDSRDKWQAVYQDQLGLVDHGLTTSLWKAPGL
HHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA