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The map label for this gene is epaO2 [H]

Identifier: 157154771

GI number: 157154771

Start: 3199703

End: 3200275

Strand: Reverse

Name: epaO2 [H]

Synonym: EcE24377A_3185

Alternate gene names: 157154771

Gene position: 3200275-3199703 (Counterclockwise)

Preceding gene: 157158665

Following gene: 157159039

Centisome position: 64.27

GC content: 31.24

Gene sequence:

>573_bases
TTGAATCTTTGGTTAGATAAAGTCACAGTCTGCCAATACGGAAACGCTCCAGCGTTAGACAAAAAATCATTATATTGGTC
CATTCATTTTGTAATTGGATTTAGTAAAACATGTTACAGAAGTCTTGTTGATATTGAAGTTGGTGATGTTTTATTAATCT
CAAATAATTTGGCTTATGCGGTTATTTATAATACAAAAATTTGTGATTTAATTTATCCAGAGGAGTTAAAAATGGCTGAT
CATTTTGAGTACGAGGAAGATTTTGAAACAGATGATTTTGATATCAAAAAAAACGAGAGTGAGATTTATGATGAAAATGA
CGATCAGATGATTAATAGTTTTGAAGACTTGCCTGTAAAAATTGAGTTTGTTCTTGGTAAGAAAATAATGAATCTATATG
AAATAGACGAACTCTGTGCAAAAAGAATAATATCTCTGCTTCCTGAATCTGAGAAAAATATAAAAATACGTGTAAATGGC
GCGCTAACTGGCTATGGCGAACTTGTTGAAGTGGATGATAAATTGGGCGTAGAAATTCATTCTTGGTTATCAGGGAATAA
TAATGTCAAATAG

Upstream 100 bases:

>100_bases
AAAGCATCCTGAACTAAAATATAATCGTTTGCACGTAGGTAAAGTATTTGATGGAGTATTGAGCCCTGCTCAACCACTAA
TAAGAATATGAACAGGGGAA

Downstream 100 bases:

>100_bases
CATTTCGCTGATAGCGATATTATCATTATTTACACTATTGCCGTTTATAATAGCATCCGGGACATGTTTTATTAAATTCT
CGATTGTTTTTGTTATTGTC

Product: type III secretion apparatus protein EpaO2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFSKTCYRSLVDIEVGDVLLISNNLAYAVIYNTKICDLIYPEELKMAD
HFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIKIRVNG
ALTGYGELVEVDDKLGVEIHSWLSGNNNVK

Sequences:

>Translated_190_residues
MNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFSKTCYRSLVDIEVGDVLLISNNLAYAVIYNTKICDLIYPEELKMAD
HFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIKIRVNG
ALTGYGELVEVDDKLGVEIHSWLSGNNNVK
>Mature_190_residues
MNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFSKTCYRSLVDIEVGDVLLISNNLAYAVIYNTKICDLIYPEELKMAD
HFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIKIRVNG
ALTGYGELVEVDDKLGVEIHSWLSGNNNVK

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001543
- InterPro:   IPR003283
- InterPro:   IPR013385 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 21921; Mature: 21921

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFSKTCYRSLVDIEVGDVLLISNNLAYA
CCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHCCCCCCEEEEECCEEEE
VIYNTKICDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVK
EEECCEEEEEECCHHHCHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCEE
IEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIKIRVNGALTGYGELVEVDDKLGVEIH
EEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCEEEECCCCCEEEE
SWLSGNNNVK
EEECCCCCCC
>Mature Secondary Structure
MNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFSKTCYRSLVDIEVGDVLLISNNLAYA
CCCCCCEEEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHCCCCCCEEEEECCEEEE
VIYNTKICDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFEDLPVK
EEECCEEEEEECCHHHCHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCEE
IEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIKIRVNGALTGYGELVEVDDKLGVEIH
EEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCEEEECCCCCEEEE
SWLSGNNNVK
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7638176 [H]