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The map label for this gene is putA [C]

Identifier: 156978291

GI number: 156978291

Start: 2171240

End: 2171947

Strand: Direct

Name: putA [C]

Synonym: VIBHAR_07098

Alternate gene names: 156978291

Gene position: 2171240-2171947 (Clockwise)

Preceding gene: 156978289

Following gene: 156978292

Centisome position: 98.51

GC content: 47.88

Gene sequence:

>708_bases
ATGGTTCATCAGATCACGTTTTTTAAAGATGCATTCTCTGCGTGGGAGCAGTGGAATCTGACCGATTTTGATTACAAATG
CGAGCATTTACTGGCATTTAAATCTGCCATTGAAGAGCAGCATAGCTTGGTTGGCAAAGTGGTGTCTTACCACTTACAGC
AAGCGTCGTCTCTGTTGGCAGAAACTCATCAGTTAGTGGGCCCTACGGGTGAAACTAACGAGCTGTACACAGCGGGTCGC
GGTGTGGCTTTGGTCGTTTGTGAAAACAACCTAGAACACACAGAGAACGCACTGTACAGCGCATTTGCAATGATCACCTG
TGCTTTGATTGCAGGCAACAGTGTGGTGATTTGCAGTGACAATGCAGAGCTTAATCAATTGGTTAAGCAGGCTGTAGCGT
CAGCAAACTTCCCATCTAACTTAGTCCAAGTGGTTGCGTACGATGCGTCTTCCCCGTTGTTAGACAGTGACGTACGTAGT
GTGGGTTATGTTGGGCATTCACAGGTTGAGCACGCACTTAACCTGCAACTGGCTAAGCGTGACGGTGCCATCGTCGGCTT
AGTTTCTGAAACGGATTTAGAGTCACCGACATTGACTCATGATCCTCATCTGTCGTTGCGCTTCATTACTGAGCGTACTC
GAACGATAAATATTACAGCAGTGGGCGGTAACGCGACTCTACTCGAGTTGGGAAGCGACTCCCACTAA

Upstream 100 bases:

>100_bases
GTTTGTCGGGTACGGGTCCGAAGGCTGGTGGTCCTCACTACCTATACCGCTTTACTAAACAACATCTTGCTCAAGCAGAG
AAGGCGTAAGGAGTCGCATT

Downstream 100 bases:

>100_bases
CCTGACTTGTGATACCAATCTGAATCAAAAGGGCCTGAGAGCTTACTCGGGTTGGTATCTCTTGCTTTGATGTATTGCTG
CGATGGCAAAATATCAAAGA

Product: delta 1-pyrroline-5-carboxylate dehydrogenase

Products: 1-Pyrroline-5-carboxylate; FADH2; H+ [C]

Alternate protein names: Delta 1-Pyrroline-5-Carboxylate Dehydrogenase; Delta 1-Pyrroline-5-Carboxylate Dehydrogenase-Like Protein

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MVHQITFFKDAFSAWEQWNLTDFDYKCEHLLAFKSAIEEQHSLVGKVVSYHLQQASSLLAETHQLVGPTGETNELYTAGR
GVALVVCENNLEHTENALYSAFAMITCALIAGNSVVICSDNAELNQLVKQAVASANFPSNLVQVVAYDASSPLLDSDVRS
VGYVGHSQVEHALNLQLAKRDGAIVGLVSETDLESPTLTHDPHLSLRFITERTRTINITAVGGNATLLELGSDSH

Sequences:

>Translated_235_residues
MVHQITFFKDAFSAWEQWNLTDFDYKCEHLLAFKSAIEEQHSLVGKVVSYHLQQASSLLAETHQLVGPTGETNELYTAGR
GVALVVCENNLEHTENALYSAFAMITCALIAGNSVVICSDNAELNQLVKQAVASANFPSNLVQVVAYDASSPLLDSDVRS
VGYVGHSQVEHALNLQLAKRDGAIVGLVSETDLESPTLTHDPHLSLRFITERTRTINITAVGGNATLLELGSDSH
>Mature_235_residues
MVHQITFFKDAFSAWEQWNLTDFDYKCEHLLAFKSAIEEQHSLVGKVVSYHLQQASSLLAETHQLVGPTGETNELYTAGR
GVALVVCENNLEHTENALYSAFAMITCALIAGNSVVICSDNAELNQLVKQAVASANFPSNLVQVVAYDASSPLLDSDVRS
VGYVGHSQVEHALNLQLAKRDGAIVGLVSETDLESPTLTHDPHLSLRFITERTRTINITAVGGNATLLELGSDSH

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.99.8; 1.5.1.12 [C]

Molecular weight: Translated: 25522; Mature: 25522

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHQITFFKDAFSAWEQWNLTDFDYKCEHLLAFKSAIEEQHSLVGKVVSYHLQQASSLLA
CCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETHQLVGPTGETNELYTAGRGVALVVCENNLEHTENALYSAFAMITCALIAGNSVVICSD
HHHHHCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
NAELNQLVKQAVASANFPSNLVQVVAYDASSPLLDSDVRSVGYVGHSQVEHALNLQLAKR
CHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHEEEEEC
DGAIVGLVSETDLESPTLTHDPHLSLRFITERTRTINITAVGGNATLLELGSDSH
CCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure
MVHQITFFKDAFSAWEQWNLTDFDYKCEHLLAFKSAIEEQHSLVGKVVSYHLQQASSLLA
CCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETHQLVGPTGETNELYTAGRGVALVVCENNLEHTENALYSAFAMITCALIAGNSVVICSD
HHHHHCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECC
NAELNQLVKQAVASANFPSNLVQVVAYDASSPLLDSDVRSVGYVGHSQVEHALNLQLAKR
CHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHEEEEEC
DGAIVGLVSETDLESPTLTHDPHLSLRFITERTRTINITAVGGNATLLELGSDSH
CCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 45 {@PROL01.txt*L-proline!}} [C]

Substrates: FAD; L-Proline [C]

Specific reaction: FAD + L-Proline --> 1-Pyrroline-5-carboxylate + FADH2 + H+ [C]

General reaction: Redox reaction [C]

Inhibitor: L-Azetidine-2-carboxylate; Lactate; Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA