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The map label for this gene is thiE

Identifier: 156978040

GI number: 156978040

Start: 1954994

End: 1955608

Strand: Direct

Name: thiE

Synonym: VIBHAR_06844

Alternate gene names: 156978040

Gene position: 1954994-1955608 (Clockwise)

Preceding gene: 156978039

Following gene: 156978041

Centisome position: 88.7

GC content: 47.48

Gene sequence:

>615_bases
ATGAACGCCTATCGTTTATATTTGGTCACCGATGACCAACAAGATTTAGCCACACTAAAGCGTGTTGTGAGAAAAGCCGT
GGAAGGTGGCGTGACCATGGTACAAGTTCGCGAAAAACACGGTGATGTGCGTGCGTTTATCGAACGAGCTCAAGCCGTCA
AAGACATCCTTAAAGATACCGATGTGCCTTTGATCATCAATGACCGCGTAGATGTCGCACTGGCTGTCGATGCTGATGGG
GTTCACCTCGGACAATCAGACATGCCAGCAACCATTGCTCGTGAGTTGATTGGACCAAACAAAATTCTCGGCTTATCTAT
CGAAAATGAAGAGCAGCTTACAGAAGCCGACTCTCTTCCGATTGATTACATCGGTTTGAGCGCTATCTTCGCAACACCAA
CCAAAACCAACACCAAGAAGCACTGGGGTATTGATGGGTTGAAAATGGCGTTAGAGACGACTTCACTGCCGATTGTTGCT
ATTGGCGGCATCAACGAAAGTAACATTCCTCAACTGAGCGCAACTGGTGTTCATGGCTTGGCTTTGGTTTCTGCAATTTG
CCACGCGGAAGATCCAAAAGCCGCATCGGAATATCTGTTAGGGTTGATGCGCTAA

Upstream 100 bases:

>100_bases
AAACGCTGGCCAAACGCCTAAAAATGCAAGTGAGCTAAGCTTTTCTCTAACAAATACTGGCCTCTCATTCCATCTAGATG
AGAGGCCCAAAGGAAACAAA

Downstream 100 bases:

>100_bases
CCTTGCTCACTCCCGAACAAAGCGCTTATGGATGAGCGCGTTCACTTCTCAAAACTTTCAATAGTCCCTCCTTTCACAAC
GAAGACTTGCGATGTCACTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNAYRLYLVTDDQQDLATLKRVVRKAVEGGVTMVQVREKHGDVRAFIERAQAVKDILKDTDVPLIINDRVDVALAVDADG
VHLGQSDMPATIARELIGPNKILGLSIENEEQLTEADSLPIDYIGLSAIFATPTKTNTKKHWGIDGLKMALETTSLPIVA
IGGINESNIPQLSATGVHGLALVSAICHAEDPKAASEYLLGLMR

Sequences:

>Translated_204_residues
MNAYRLYLVTDDQQDLATLKRVVRKAVEGGVTMVQVREKHGDVRAFIERAQAVKDILKDTDVPLIINDRVDVALAVDADG
VHLGQSDMPATIARELIGPNKILGLSIENEEQLTEADSLPIDYIGLSAIFATPTKTNTKKHWGIDGLKMALETTSLPIVA
IGGINESNIPQLSATGVHGLALVSAICHAEDPKAASEYLLGLMR
>Mature_204_residues
MNAYRLYLVTDDQQDLATLKRVVRKAVEGGVTMVQVREKHGDVRAFIERAQAVKDILKDTDVPLIINDRVDVALAVDADG
VHLGQSDMPATIARELIGPNKILGLSIENEEQLTEADSLPIDYIGLSAIFATPTKTNTKKHWGIDGLKMALETTSLPIVA
IGGINESNIPQLSATGVHGLALVSAICHAEDPKAASEYLLGLMR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=198, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=42.2727272727273, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_VIBHB (A7N7S3)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001448946.1
- ProteinModelPortal:   A7N7S3
- SMR:   A7N7S3
- STRING:   A7N7S3
- GeneID:   5557697
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_06844
- NMPDR:   fig|338187.4.peg.4064
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22005; Mature: 22005

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAYRLYLVTDDQQDLATLKRVVRKAVEGGVTMVQVREKHGDVRAFIERAQAVKDILKDT
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCC
DVPLIINDRVDVALAVDADGVHLGQSDMPATIARELIGPNKILGLSIENEEQLTEADSLP
CCCEEEECCCEEEEEECCCCEEECCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHCCCC
IDYIGLSAIFATPTKTNTKKHWGIDGLKMALETTSLPIVAIGGINESNIPQLSATGVHGL
CHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHH
ALVSAICHAEDPKAASEYLLGLMR
HHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNAYRLYLVTDDQQDLATLKRVVRKAVEGGVTMVQVREKHGDVRAFIERAQAVKDILKDT
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCC
DVPLIINDRVDVALAVDADGVHLGQSDMPATIARELIGPNKILGLSIENEEQLTEADSLP
CCCEEEECCCEEEEEECCCCEEECCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHCCCC
IDYIGLSAIFATPTKTNTKKHWGIDGLKMALETTSLPIVAIGGINESNIPQLSATGVHGL
CHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHH
ALVSAICHAEDPKAASEYLLGLMR
HHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA