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The map label for this gene is thiM

Identifier: 156978039

GI number: 156978039

Start: 1954140

End: 1954931

Strand: Direct

Name: thiM

Synonym: VIBHAR_06843

Alternate gene names: 156978039

Gene position: 1954140-1954931 (Clockwise)

Preceding gene: 156978038

Following gene: 156978040

Centisome position: 88.66

GC content: 50.88

Gene sequence:

>792_bases
ATGTTAATTGAACAAATCACCCAAGCATTAACGGCAGTACGCCAGCAAAAGCCATTAGTCGTCAACATCACTAACTACGT
GGTGATGAACAACACGGCAAACGCGCTATTGGCTATCGGCGCTTCTCCAATCATGGCGCACTCCAAGCAAGAAATGGTAG
AGATGATGTCGTTCGCGGGCGCACTGGTTATCAACATCGGTACCCTAGATAGCGTTTGGACACCGCGTATGAGCTATGCC
GTAGAGCAAGCCAATGCAAATGGTAAAATCGTTGTACTCGACCCCGTCGGTTGTGGAGCAAGCTCATTACGTACCGAGAC
ATCACGTGAGATCGCACGCCTTGCAGACAAGTTGATCATTCGTGGTAACGCCTCAGAAATCATTGCACTCGCTGGCGAGC
AAGCACAAAGCAAAGGCGTTGATGCGTTAGACAGCAGCGACGCAGCACTTGGCGCAGCCAACTTCTTAGTCGCAGAATAT
GGTGCAAGTGTGGTGATTTCTGGCGAGACTGATTACATCGTCACGAAAGGGCAAACTGTGCAACTGAACAACGGTCACGA
GATGATGCCATACGTTACCGGAATGGGCTGTACACTATCGGCTCTGACAGGCGCTTTTGCAGCTGTTGGTGATGATACCG
GCCTTGCTGCAGCGGCGGTACTGGGCGTTGTTGGCGAGATTGCAGCAGAACAAGCTCGCGGCCCAGGTAGCCTGCAAATG
AACTTGTTGGATGAGCTTTATCAGTTGGATGAAGAAACGCTGGCCAAACGCCTAAAAATGCAAGTGAGCTAA

Upstream 100 bases:

>100_bases
TGGTCAAAACCTGATTCAGGTATTCAAAATCGCAACACGTATGGAAGTGGCATTCTGGCAACAAGGCCTAAACGCTCAGC
AAGATTAATCGGAGAGTCTC

Downstream 100 bases:

>100_bases
GCTTTTCTCTAACAAATACTGGCCTCTCATTCCATCTAGATGAGAGGCCCAAAGGAAACAAAATGAACGCCTATCGTTTA
TATTTGGTCACCGATGACCA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLIEQITQALTAVRQQKPLVVNITNYVVMNNTANALLAIGASPIMAHSKQEMVEMMSFAGALVINIGTLDSVWTPRMSYA
VEQANANGKIVVLDPVGCGASSLRTETSREIARLADKLIIRGNASEIIALAGEQAQSKGVDALDSSDAALGAANFLVAEY
GASVVISGETDYIVTKGQTVQLNNGHEMMPYVTGMGCTLSALTGAFAAVGDDTGLAAAAVLGVVGEIAAEQARGPGSLQM
NLLDELYQLDEETLAKRLKMQVS

Sequences:

>Translated_263_residues
MLIEQITQALTAVRQQKPLVVNITNYVVMNNTANALLAIGASPIMAHSKQEMVEMMSFAGALVINIGTLDSVWTPRMSYA
VEQANANGKIVVLDPVGCGASSLRTETSREIARLADKLIIRGNASEIIALAGEQAQSKGVDALDSSDAALGAANFLVAEY
GASVVISGETDYIVTKGQTVQLNNGHEMMPYVTGMGCTLSALTGAFAAVGDDTGLAAAAVLGVVGEIAAEQARGPGSLQM
NLLDELYQLDEETLAKRLKMQVS
>Mature_263_residues
MLIEQITQALTAVRQQKPLVVNITNYVVMNNTANALLAIGASPIMAHSKQEMVEMMSFAGALVINIGTLDSVWTPRMSYA
VEQANANGKIVVLDPVGCGASSLRTETSREIARLADKLIIRGNASEIIALAGEQAQSKGVDALDSSDAALGAANFLVAEY
GASVVISGETDYIVTKGQTVQLNNGHEMMPYVTGMGCTLSALTGAFAAVGDDTGLAAAAVLGVVGEIAAEQARGPGSLQM
NLLDELYQLDEETLAKRLKMQVS

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=40.1574803149606, Blast_Score=151, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6325042, Length=283, Percent_Identity=28.9752650176678, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_VIBHB (A7N7S2)

Other databases:

- EMBL:   CP000790
- RefSeq:   YP_001448945.1
- ProteinModelPortal:   A7N7S2
- SMR:   A7N7S2
- STRING:   A7N7S2
- GeneID:   5557696
- GenomeReviews:   CP000790_GR
- KEGG:   vha:VIBHAR_06843
- NMPDR:   fig|338187.4.peg.4063
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27503; Mature: 27503

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: BINDING 45-45 BINDING 121-121 BINDING 167-167 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEQITQALTAVRQQKPLVVNITNYVVMNNTANALLAIGASPIMAHSKQEMVEMMSFAG
CCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHCC
ALVINIGTLDSVWTPRMSYAVEQANANGKIVVLDPVGCGASSLRTETSREIARLADKLII
EEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEE
RGNASEIIALAGEQAQSKGVDALDSSDAALGAANFLVAEYGASVVISGETDYIVTKGQTV
CCCCCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCEE
QLNNGHEMMPYVTGMGCTLSALTGAFAAVGDDTGLAAAAVLGVVGEIAAEQARGPGSLQM
EECCCCEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEH
NLLDELYQLDEETLAKRLKMQVS
HHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLIEQITQALTAVRQQKPLVVNITNYVVMNNTANALLAIGASPIMAHSKQEMVEMMSFAG
CCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCHHHHCCHHHHHHHHHHCC
ALVINIGTLDSVWTPRMSYAVEQANANGKIVVLDPVGCGASSLRTETSREIARLADKLII
EEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEE
RGNASEIIALAGEQAQSKGVDALDSSDAALGAANFLVAEYGASVVISGETDYIVTKGQTV
CCCCCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCEE
QLNNGHEMMPYVTGMGCTLSALTGAFAAVGDDTGLAAAAVLGVVGEIAAEQARGPGSLQM
EECCCCEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEH
NLLDELYQLDEETLAKRLKMQVS
HHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA