Click here to switch to the map view.

The map label for this gene is pspE [H]

Identifier: 156977818

GI number: 156977818

Start: 1756022

End: 1756372

Strand: Direct

Name: pspE [H]

Synonym: VIBHAR_06606

Alternate gene names: 156977818

Gene position: 1756022-1756372 (Clockwise)

Preceding gene: 156977817

Following gene: 156977819

Centisome position: 79.67

GC content: 45.01

Gene sequence:

>351_bases
ATGAAAAAACTACTTCCGATTGTATTCACTTGTTTGAGCTTAGCTTTCACCGCACCATCGGTATTTGCTTCTGAACGTGC
AGCGCAGGGTTGGCAATGGATTGAGCAAGGCGCAATGATTGTCGATGTGCGCACACCGCAAGAGTTTGCCGATGGTCACT
TGGACAATGCGGTTAACTTTCCGCTTTCAGAGTTAGATAAACACTTTGCTAATGTCGATAAAGATACGCAAATCGTACTT
TACTGCCGAAGTGGTAATCGCTCAGGGCAAGCGTATCAGTACCTACAATCACAAGGGTTTACCAACCTTCATAACGCGGG
TGGCTTAGTTGAGATGCAAAAATCGAAGTAA

Upstream 100 bases:

>100_bases
GTTACGACATTTCGACCGATGCCACATCGAAGTTTGATAATCGCACGTATAATCTTCGTCAGAACGATTAAGGGCAATTG
ACCTTGAGGAAAGGAACTCA

Downstream 100 bases:

>100_bases
CGACCAAGTTGCCGTAAAAGAGCAAAAAACCGCAGCGATCATCGACGCGTTCCTAAAACACGGTGGTCAAACAGAGCGTG
AAGCGAAAGATATCCAAGGC

Product: hypothetical protein

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MKKLLPIVFTCLSLAFTAPSVFASERAAQGWQWIEQGAMIVDVRTPQEFADGHLDNAVNFPLSELDKHFANVDKDTQIVL
YCRSGNRSGQAYQYLQSQGFTNLHNAGGLVEMQKSK

Sequences:

>Translated_116_residues
MKKLLPIVFTCLSLAFTAPSVFASERAAQGWQWIEQGAMIVDVRTPQEFADGHLDNAVNFPLSELDKHFANVDKDTQIVL
YCRSGNRSGQAYQYLQSQGFTNLHNAGGLVEMQKSK
>Mature_116_residues
MKKLLPIVFTCLSLAFTAPSVFASERAAQGWQWIEQGAMIVDVRTPQEFADGHLDNAVNFPLSELDKHFANVDKDTQIVL
YCRSGNRSGQAYQYLQSQGFTNLHNAGGLVEMQKSK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=110, Percent_Identity=35.4545454545455, Blast_Score=72, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 12937; Mature: 12937

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLPIVFTCLSLAFTAPSVFASERAAQGWQWIEQGAMIVDVRTPQEFADGHLDNAVNF
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCC
PLSELDKHFANVDKDTQIVLYCRSGNRSGQAYQYLQSQGFTNLHNAGGLVEMQKSK
CHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MKKLLPIVFTCLSLAFTAPSVFASERAAQGWQWIEQGAMIVDVRTPQEFADGHLDNAVNF
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCC
PLSELDKHFANVDKDTQIVLYCRSGNRSGQAYQYLQSQGFTNLHNAGGLVEMQKSK
CHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]