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The map label for this gene is frwC [H]

Identifier: 156977804

GI number: 156977804

Start: 1735552

End: 1736952

Strand: Direct

Name: frwC [H]

Synonym: VIBHAR_06592

Alternate gene names: 156977804

Gene position: 1735552-1736952 (Clockwise)

Preceding gene: 156977803

Following gene: 156977805

Centisome position: 78.74

GC content: 47.32

Gene sequence:

>1401_bases
ATGAGTACAATCACAGCTCAAGCTAGTAATAATAGCGACTTCAAAAAAATCCTAAGTACCATGAAAGGTCACCTTTTATT
CGGTACTTCTCACATGCTTCCATTTATCGTTGCGGGTGGTGTACTACTAGCGCTAGCGGTAATGGCATCAGGTAAAGGTG
CGGTTCCTGCGGACGGTTTATTGGCTGATATTTCTAATATCGGTATTAAAGGTCTGGTGCTGTTCCCAATTATTCTTGGT
GGCTTCATCGGTTACTCAATTGCAGACAAACCTGCTCTAGCACCAGCGATGATCTCTTCTGGCATCATGGCTGACATGGG
CGGCGGTTTCCTTGGTTGTATCGTAGCGGGTTTCATCGCAGGTGGTGTGGTATTCCAACTTAAGAAGATCCCTCTTTCTG
CTAACATGACTGCGTTGGGCGCTTACTTCATCTACCCTCTACTAGGTACGCTTATCTCTGCAGGTATCGTTCTATGGGGT
ATCGGTGAGCCAATCAAGATCTTCATGGCTTCTATGAACGAATTCCTAGCTTCTATGGCTGGCGCTTCTAAAGTGGTTCT
AGGTACGATTCTTGGTGGTATGACTGCATTCGATATGGGCGGTCCAATCAACAAAGTAGCAACGCTATTCGCTCAAACTC
AAGTTGATACTCAACCATGGCTAATGGGTGGTGTTGGTATCGCAATCTGTACGCCTCCTCTAGGTATGGCACTAGCAACA
TTCCTATTCAAGAAGAAGTTCACTAAGCAAGAACAAGAAGCAGGTAAAGCAGCAGCAATCATGGGTTCTATCGGTATCTC
TGAAGGTGCTATCCCATTCGCTGCAAACGACCCAGTACGCGTTCTTCCTTCAATCGTTGCTGGTGGTATCGTTGGCTGTG
TGTTCGGCTTCCTAACAGACGTACTGCTACACGCACCATGGGGCGGTCTAATCACAGCTCCTGTATCAAGCAACATCCCA
ATGTACGTTGTGGGTATCGCACTAGGCTCACTAACAACAGCGGTTATCGTTGGCTTCTGGAAACCAGTTGCTGAAGAAGA
AGCAGAAGACGAAATCGCAGAAGCAGCACCTGCACAAGCTCAAGCGGCTCCAGCAGCAGGCGAAGGCGAGTACGACATCG
TAGCAGTAACATGTTGCCCATCTGGCGTAGCACACACATTCATGGCAGCGAAAGCACTAGAAAAAGCAGGTGCAGCAGCG
GGTCTGAAAATCAAAGTAGAAACTCAAGGTCAAAACGGTATCCAGAACCGCATCACTGACCTAGATGTAGCAAACGCGAA
GCTGGTTATTCTAGCTCACGATATCCAAGTAAAAGACGCTCACCGTTTTGCAAAAGCAAACGTTGTTGAGTGTTCAACGA
AAGAAGCGATGCGTAACGCAGCGACTCTTGTGAAAGCTTAA

Upstream 100 bases:

>100_bases
TATTCGGTAAATTTAGCGAAGAGTTTATTGAAGATATTCATAACGCAAAAGACGAAAACCAAATACTAAATATTTTAATT
AATTCATAAGGCGTGAAATT

Downstream 100 bases:

>100_bases
ACCTTAAGCTCCTCCCCCTAAGAGCCCTTTCCCCCAAAGGGCTCTATTTCAAGTTTTTAAGTTTTAGTAATACCCCTGAT
TTTTTTAATTATATAACCCT

Product: PTS system fructose-specific IIBC component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system fructose-like EIIC component 2 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSTITAQASNNSDFKKILSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIILG
GFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLLGTLISAGIVLWG
IGEPIKIFMASMNEFLASMAGASKVVLGTILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALAT
FLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPVRVLPSIVAGGIVGCVFGFLTDVLLHAPWGGLITAPVSSNIP
MYVVGIALGSLTTAVIVGFWKPVAEEEAEDEIAEAAPAQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAA
GLKIKVETQGQNGIQNRITDLDVANAKLVILAHDIQVKDAHRFAKANVVECSTKEAMRNAATLVKA

Sequences:

>Translated_466_residues
MSTITAQASNNSDFKKILSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIILG
GFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLLGTLISAGIVLWG
IGEPIKIFMASMNEFLASMAGASKVVLGTILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALAT
FLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPVRVLPSIVAGGIVGCVFGFLTDVLLHAPWGGLITAPVSSNIP
MYVVGIALGSLTTAVIVGFWKPVAEEEAEDEIAEAAPAQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAA
GLKIKVETQGQNGIQNRITDLDVANAKLVILAHDIQVKDAHRFAKANVVECSTKEAMRNAATLVKA
>Mature_465_residues
STITAQASNNSDFKKILSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGLLADISNIGIKGLVLFPIILGG
FIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIAGGVVFQLKKIPLSANMTALGAYFIYPLLGTLISAGIVLWGI
GEPIKIFMASMNEFLASMAGASKVVLGTILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALATF
LFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPVRVLPSIVAGGIVGCVFGFLTDVLLHAPWGGLITAPVSSNIPM
YVVGIALGSLTTAVIVGFWKPVAEEEAEDEIAEAAPAQAQAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAAG
LKIKVETQGQNGIQNRITDLDVANAKLVILAHDIQVKDAHRFAKANVVECSTKEAMRNAATLVKA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790386, Length=308, Percent_Identity=46.7532467532467, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788492, Length=355, Percent_Identity=38.0281690140845, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786951, Length=315, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87082348, Length=356, Percent_Identity=31.7415730337079, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788729, Length=386, Percent_Identity=27.4611398963731, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=52.2727272727273, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788730, Length=93, Percent_Identity=45.1612903225806, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790390, Length=94, Percent_Identity=40.4255319148936, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013014
- InterPro:   IPR006327 [H]

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 47675; Mature: 47544

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTITAQASNNSDFKKILSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGL
CCCEEECCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LADISNIGIKGLVLFPIILGGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIA
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHH
GGVVFQLKKIPLSANMTALGAYFIYPLLGTLISAGIVLWGIGEPIKIFMASMNEFLASMA
CCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHC
GASKVVLGTILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALAT
CCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHH
FLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPVRVLPSIVAGGIVGCVFGFLTD
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLLHAPWGGLITAPVSSNIPMYVVGIALGSLTTAVIVGFWKPVAEEEAEDEIAEAAPAQA
HHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAAGLKIKVETQGQNGIQNRITD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCC
LDVANAKLVILAHDIQVKDAHRFAKANVVECSTKEAMRNAATLVKA
CCCCCEEEEEEEECCEEHHHHHHHHCCCEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STITAQASNNSDFKKILSTMKGHLLFGTSHMLPFIVAGGVLLALAVMASGKGAVPADGL
CCEEECCCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LADISNIGIKGLVLFPIILGGFIGYSIADKPALAPAMISSGIMADMGGGFLGCIVAGFIA
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHH
GGVVFQLKKIPLSANMTALGAYFIYPLLGTLISAGIVLWGIGEPIKIFMASMNEFLASMA
CCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHC
GASKVVLGTILGGMTAFDMGGPINKVATLFAQTQVDTQPWLMGGVGIAICTPPLGMALAT
CCHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHH
FLFKKKFTKQEQEAGKAAAIMGSIGISEGAIPFAANDPVRVLPSIVAGGIVGCVFGFLTD
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLLHAPWGGLITAPVSSNIPMYVVGIALGSLTTAVIVGFWKPVAEEEAEDEIAEAAPAQA
HHHHCCCCCEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QAAPAAGEGEYDIVAVTCCPSGVAHTFMAAKALEKAGAAAGLKIKVETQGQNGIQNRITD
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCC
LDVANAKLVILAHDIQVKDAHRFAKANVVECSTKEAMRNAATLVKA
CCCCCEEEEEEEECCEEHHHHHHHHCCCEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]