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The map label for this gene is rhtB [H]

Identifier: 156977580

GI number: 156977580

Start: 1542432

End: 1543055

Strand: Reverse

Name: rhtB [H]

Synonym: VIBHAR_06368

Alternate gene names: 156977580

Gene position: 1543055-1542432 (Counterclockwise)

Preceding gene: 156977584

Following gene: 156977574

Centisome position: 70.01

GC content: 38.46

Gene sequence:

>624_bases
ATGGATTATAACCTTTGGATTGCTTTTTTTATCGCTTCTCTGATGCTTGCTATTTCCCCCGGAGCGGGTGCCATAAATAG
TATGTCTATTACGATGAAATATGGCCTAAAAACGAGTTTGATAGCGAATTTAGGCCTGCAGTGTGGGAACGTATTTAATA
TCACAGTTGTTGGTATTGGGCTTGGGGCATTATTAGTCAAATCAGAAGTGTTATTCTCGTTGATAAAGTGGGTTGGGGTA
GGTTATCTGATTTATTTAGGTATCAAAAAGTTTAAAGAGCTGCCAGAACTTGCTGATAAAGCAGAATCGATAAATACACA
AAGCTCTTCTAAATTGTTTTTTCAATCGATATTGGTCAACATTACTAACCCAAAAAGTATCGTTTTTTTGATTGCACTGT
TACCGCAGTTTATTGTTCCTACATACTCTTATCACGAGCAATTACTGATATTAGGTACGACCTTGATTCTCATTGATGGA
ACTGTGATGTTTGGCTATTCATTGGTTGCTAGTCGTATTGCCAAGTCAGTTCAAGAGCCGAAAAATGCCGTCATATTGAA
TCGAATTTTTGGCAGTATGTTTGTTGGGGCAGGTACTCTACTGGCCTTTGGCAGCAGAAGCTAG

Upstream 100 bases:

>100_bases
TTACGAATGAACATGCTAAAACATAGTTTTTCTTTTCTATGTGTGATTTTAACATACTGACAGCTCGCAGTGTTCGTTTT
ACAGCTTATGGAGTTTTACT

Downstream 100 bases:

>100_bases
TATCAGGTGCGGGATTTATACTGTTTAAAAAAGGAGCTGAACTGTCAGCTCCTTCGTTTTTATAGGCTCTTATTTACAGT
TCAATCTTTTTAACCATTCC

Product: putative threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MDYNLWIAFFIASLMLAISPGAGAINSMSITMKYGLKTSLIANLGLQCGNVFNITVVGIGLGALLVKSEVLFSLIKWVGV
GYLIYLGIKKFKELPELADKAESINTQSSSKLFFQSILVNITNPKSIVFLIALLPQFIVPTYSYHEQLLILGTTLILIDG
TVMFGYSLVASRIAKSVQEPKNAVILNRIFGSMFVGAGTLLAFGSRS

Sequences:

>Translated_207_residues
MDYNLWIAFFIASLMLAISPGAGAINSMSITMKYGLKTSLIANLGLQCGNVFNITVVGIGLGALLVKSEVLFSLIKWVGV
GYLIYLGIKKFKELPELADKAESINTQSSSKLFFQSILVNITNPKSIVFLIALLPQFIVPTYSYHEQLLILGTTLILIDG
TVMFGYSLVASRIAKSVQEPKNAVILNRIFGSMFVGAGTLLAFGSRS
>Mature_207_residues
MDYNLWIAFFIASLMLAISPGAGAINSMSITMKYGLKTSLIANLGLQCGNVFNITVVGIGLGALLVKSEVLFSLIKWVGV
GYLIYLGIKKFKELPELADKAESINTQSSSKLFFQSILVNITNPKSIVFLIALLPQFIVPTYSYHEQLLILGTTLILIDG
TVMFGYSLVASRIAKSVQEPKNAVILNRIFGSMFVGAGTLLAFGSRS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=43.0693069306931, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1788099, Length=202, Percent_Identity=24.7524752475248, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22423; Mature: 22423

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYNLWIAFFIASLMLAISPGAGAINSMSITMKYGLKTSLIANLGLQCGNVFNITVVGIG
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEEHHH
LGALLVKSEVLFSLIKWVGVGYLIYLGIKKFKELPELADKAESINTQSSSKLFFQSILVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITNPKSIVFLIALLPQFIVPTYSYHEQLLILGTTLILIDGTVMFGYSLVASRIAKSVQEP
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHCC
KNAVILNRIFGSMFVGAGTLLAFGSRS
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDYNLWIAFFIASLMLAISPGAGAINSMSITMKYGLKTSLIANLGLQCGNVFNITVVGIG
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEEHHH
LGALLVKSEVLFSLIKWVGVGYLIYLGIKKFKELPELADKAESINTQSSSKLFFQSILVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITNPKSIVFLIALLPQFIVPTYSYHEQLLILGTTLILIDGTVMFGYSLVASRIAKSVQEP
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHCC
KNAVILNRIFGSMFVGAGTLLAFGSRS
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]