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The map label for this gene is opuAB [H]

Identifier: 156977390

GI number: 156977390

Start: 1356049

End: 1356891

Strand: Reverse

Name: opuAB [H]

Synonym: VIBHAR_06177

Alternate gene names: 156977390

Gene position: 1356891-1356049 (Counterclockwise)

Preceding gene: 156977391

Following gene: 156977389

Centisome position: 61.56

GC content: 48.28

Gene sequence:

>843_bases
GTGAATTTTATTACGGACAACAAACTTCCGTTGGGCGAATGGATGGAAACGGGCGTGGATTGGTTAACCATGAACGCGTC
GGGTTTCTTTGATGCTATCTCTATTTTTCTCGAAACCATCATTTTATTCGTGGTGGATATCTTTAAATGGATGCCACCGG
CAGCACCAATCATCATGACCGCAGCGATTGCGTGGTTCCTACACCGCAGCATTCCTTTAGTTGTGTTTATTGTGCTAGCG
CTGCTGACTATCCTTAACCTCGGCTACTGGCAAGAAATGCTGGAAACCTTTGTTCTCGTCTTCGCTGCGACAACGATTTC
CGTATTAATTGGCGTGCCGCTTGGCATCATGGCGGCGCATCGCCCGTGGTTGTACACCTTATTGCGCCCGATACTCGATT
TAATGCAGACGGTGCCAACGTTTGTGTATCTGATCCCAACATTGGTTCTGTTTGGTTTGGGTATCGTTCCGGGTCTGATT
TCTACGATTATCTTTGCCATCGCCGCGCCGATTCGTCTGACTTACCTTGGCATCACCAAAGTGCCAGAAGAGTTAATTGA
AGCGGGTAAAGCCTTTGGTGCTAGCCGCATGAAATTGCTATTCAAAGTCGAACTCCCAGCAGCATTGCCAAGCATTATGG
CGGGCGTTACGCAGTGCATTATGTTGTCACTTTCTATGGTGGTGATTGCTGCGCTAGTCGGTGCCGATGGCTTGGGTAAA
CCAGTCGTACGAGCACTGAACACGGTGAACATTTCTCAAGGCTTTGAAGCCGGTTTAGCGATTGTTTTGGTCGCGATTAT
TCTCGACCGTTTGTGCAAAGCACCAAACCAAAAGGAGGCATAA

Upstream 100 bases:

>100_bases
AACAAGCGATTACTTCTTACCTAAAAACAAACGCTTAATCATTCCTCTCTTTAGGAAAAGGTGGGTAGTGATGCCCACCT
TTTAACACAAAGGTTTTATT

Downstream 100 bases:

>100_bases
CATGGATGCAATTACGATTGAAAACCTCGACGTTGTGTTTGGTCAGCAGCAAGAGCAAGCATTGGCGCTTTTAGACCAAG
GTAAGTCTCGCCAAGAGATC

Product: glycine betaine/L-proline ABC transporter permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MNFITDNKLPLGEWMETGVDWLTMNASGFFDAISIFLETIILFVVDIFKWMPPAAPIIMTAAIAWFLHRSIPLVVFIVLA
LLTILNLGYWQEMLETFVLVFAATTISVLIGVPLGIMAAHRPWLYTLLRPILDLMQTVPTFVYLIPTLVLFGLGIVPGLI
STIIFAIAAPIRLTYLGITKVPEELIEAGKAFGASRMKLLFKVELPAALPSIMAGVTQCIMLSLSMVVIAALVGADGLGK
PVVRALNTVNISQGFEAGLAIVLVAIILDRLCKAPNQKEA

Sequences:

>Translated_280_residues
MNFITDNKLPLGEWMETGVDWLTMNASGFFDAISIFLETIILFVVDIFKWMPPAAPIIMTAAIAWFLHRSIPLVVFIVLA
LLTILNLGYWQEMLETFVLVFAATTISVLIGVPLGIMAAHRPWLYTLLRPILDLMQTVPTFVYLIPTLVLFGLGIVPGLI
STIIFAIAAPIRLTYLGITKVPEELIEAGKAFGASRMKLLFKVELPAALPSIMAGVTQCIMLSLSMVVIAALVGADGLGK
PVVRALNTVNISQGFEAGLAIVLVAIILDRLCKAPNQKEA
>Mature_280_residues
MNFITDNKLPLGEWMETGVDWLTMNASGFFDAISIFLETIILFVVDIFKWMPPAAPIIMTAAIAWFLHRSIPLVVFIVLA
LLTILNLGYWQEMLETFVLVFAATTISVLIGVPLGIMAAHRPWLYTLLRPILDLMQTVPTFVYLIPTLVLFGLGIVPGLI
STIIFAIAAPIRLTYLGITKVPEELIEAGKAFGASRMKLLFKVELPAALPSIMAGVTQCIMLSLSMVVIAALVGADGLGK
PVVRALNTVNISQGFEAGLAIVLVAIILDRLCKAPNQKEA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=266, Percent_Identity=42.8571428571429, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788449, Length=194, Percent_Identity=32.4742268041237, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788451, Length=234, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=34.2105263157895, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30463; Mature: 30463

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFITDNKLPLGEWMETGVDWLTMNASGFFDAISIFLETIILFVVDIFKWMPPAAPIIMT
CCCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAIAWFLHRSIPLVVFIVLALLTILNLGYWQEMLETFVLVFAATTISVLIGVPLGIMAAH
HHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPWLYTLLRPILDLMQTVPTFVYLIPTLVLFGLGIVPGLISTIIFAIAAPIRLTYLGITK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEELIEAGKAFGASRMKLLFKVELPAALPSIMAGVTQCIMLSLSMVVIAALVGADGLGK
HHHHHHHHHHHCCHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
PVVRALNTVNISQGFEAGLAIVLVAIILDRLCKAPNQKEA
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNFITDNKLPLGEWMETGVDWLTMNASGFFDAISIFLETIILFVVDIFKWMPPAAPIIMT
CCCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AAIAWFLHRSIPLVVFIVLALLTILNLGYWQEMLETFVLVFAATTISVLIGVPLGIMAAH
HHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPWLYTLLRPILDLMQTVPTFVYLIPTLVLFGLGIVPGLISTIIFAIAAPIRLTYLGITK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEELIEAGKAFGASRMKLLFKVELPAALPSIMAGVTQCIMLSLSMVVIAALVGADGLGK
HHHHHHHHHHHCCHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
PVVRALNTVNISQGFEAGLAIVLVAIILDRLCKAPNQKEA
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]