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The map label for this gene is fruB [H]

Identifier: 156977172

GI number: 156977172

Start: 1145016

End: 1146149

Strand: Direct

Name: fruB [H]

Synonym: VIBHAR_05958

Alternate gene names: 156977172

Gene position: 1145016-1146149 (Clockwise)

Preceding gene: 156977170

Following gene: 156977173

Centisome position: 51.95

GC content: 47.27

Gene sequence:

>1134_bases
ATGCTTAAGCTAAGTAAGAACGACATTACCCTATCTCAATCCGCTACAGATAAATTTGAAGCGATCAAAAGCATTGCGAA
AAGCCTGACAGACAAAGGTCTGGTTGAACAAGGCTATGTAGAAGGCATGCTAAACCGTGAAAACCAGAACTCAACGTTTC
TAGGTAACGGTATCGCGATTCCACACGGCACGACTGACACGCGCTCAATGGTGAAAACCACAGGCGTCGCTGTTCACCAC
TTCCCAGAAGGCGTTGAATGGGGCGAAGGCAACACAGTTTACGTTGCTATAGGCATTGCAGCGAAATCAGACGAGCACTT
GGGCATTCTTAAGCAGCTAACAAAAGTATTGGGTGCGGATGGCGTTGAAGAGCGTCTACGCAATGCGAAAAACGAAGACG
ACATCATTGCCCTACTTCATGGCAACGTTCAGCTAGAAGCGGATTTCGATGCTTCTCTCATTCAACTGCTATTCCCAGCA
AGCGACATGATTCAAATGAGCGCGGTTGCTGGCGGCCTACTTAAAAACACAGGCTGTACAGGTACTGAGTTCGTTGCAGA
CCTAGTAACAAAAACGCCAACGCATCTAGGCCAAGGTCTATGGCTAGTAGGTAGTGATAAGCAAGTAACTCGCACTGGTG
TTGCTTATGTCTCAACAGCAAATGATTGTGATTTCGAAGGCGAGAAAGTACGCGCTCTAGTCGCTTTTGCTGCTTGCAAC
AACGCTCACCAATCTATCCTAAGCACGATTTCAAAGCTTGTATTCAACAACGAACACAGCAAGCTGCTTAATGGCTCTGC
TGAACAAATTCTTGCGCTATTTAAAGGCGAAGAAATGGCAGCGGGAGCAACTGAAGATAGCAACACAGCAATCTTCAAAA
TCAAAAACGCACACGGCCTACACGCTCGTCCTGGTGCAATGCTAGTTGCTGAAGCGAAGAAGTTCGAATCTACAATTCGT
GTGGCAAACCTAGATGGCGACGGCAAAGCGGTAAACGCAAAGAGCCTAATGAAAGTTATCGCGCTAGGTGTGAAACACGG
TCACCAGCTTCAATTCTCTGCTGAAGGTCCAGATGCTGCACAAGCGCTTGAGTCAATCGGCAAAGCAATCGCATCTGGTC
TTGGCGAAGGTTAA

Upstream 100 bases:

>100_bases
TAAATTGAGTTCAGCAACAACAGAACCACGCGTTACGCAGCCCAAACGCTGCGTAACCAAGATGAATTAGAAATAAAGAC
CTTAAGGCTGGAGAAGCACC

Downstream 100 bases:

>100_bases
GGGGCGTGAGAATGACAGCATTGAAAACAAACAAAGTCGTTACCATCACGCTTAACCCTGCCCTTGATCTAACAGGTTCG
CTGGACTCAATTAAAGTGGG

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLKLSKNDITLSQSATDKFEAIKSIAKSLTDKGLVEQGYVEGMLNRENQNSTFLGNGIAIPHGTTDTRSMVKTTGVAVHH
FPEGVEWGEGNTVYVAIGIAAKSDEHLGILKQLTKVLGADGVEERLRNAKNEDDIIALLHGNVQLEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNTGCTGTEFVADLVTKTPTHLGQGLWLVGSDKQVTRTGVAYVSTANDCDFEGEKVRALVAFAACN
NAHQSILSTISKLVFNNEHSKLLNGSAEQILALFKGEEMAAGATEDSNTAIFKIKNAHGLHARPGAMLVAEAKKFESTIR
VANLDGDGKAVNAKSLMKVIALGVKHGHQLQFSAEGPDAAQALESIGKAIASGLGEG

Sequences:

>Translated_377_residues
MLKLSKNDITLSQSATDKFEAIKSIAKSLTDKGLVEQGYVEGMLNRENQNSTFLGNGIAIPHGTTDTRSMVKTTGVAVHH
FPEGVEWGEGNTVYVAIGIAAKSDEHLGILKQLTKVLGADGVEERLRNAKNEDDIIALLHGNVQLEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNTGCTGTEFVADLVTKTPTHLGQGLWLVGSDKQVTRTGVAYVSTANDCDFEGEKVRALVAFAACN
NAHQSILSTISKLVFNNEHSKLLNGSAEQILALFKGEEMAAGATEDSNTAIFKIKNAHGLHARPGAMLVAEAKKFESTIR
VANLDGDGKAVNAKSLMKVIALGVKHGHQLQFSAEGPDAAQALESIGKAIASGLGEG
>Mature_377_residues
MLKLSKNDITLSQSATDKFEAIKSIAKSLTDKGLVEQGYVEGMLNRENQNSTFLGNGIAIPHGTTDTRSMVKTTGVAVHH
FPEGVEWGEGNTVYVAIGIAAKSDEHLGILKQLTKVLGADGVEERLRNAKNEDDIIALLHGNVQLEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNTGCTGTEFVADLVTKTPTHLGQGLWLVGSDKQVTRTGVAYVSTANDCDFEGEKVRALVAFAACN
NAHQSILSTISKLVFNNEHSKLLNGSAEQILALFKGEEMAAGATEDSNTAIFKIKNAHGLHARPGAMLVAEAKKFESTIR
VANLDGDGKAVNAKSLMKVIALGVKHGHQLQFSAEGPDAAQALESIGKAIASGLGEG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=378, Percent_Identity=48.4126984126984, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=38.8888888888889, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39832; Mature: 39832

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLSKNDITLSQSATDKFEAIKSIAKSLTDKGLVEQGYVEGMLNRENQNSTFLGNGIAI
CCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCEEC
PHGTTDTRSMVKTTGVAVHHFPEGVEWGEGNTVYVAIGIAAKSDEHLGILKQLTKVLGAD
CCCCCHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC
GVEERLRNAKNEDDIIALLHGNVQLEADFDASLIQLLFPASDMIQMSAVAGGLLKNTGCT
CHHHHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHCCCHHHHHHHHHHCHHHHCCCCC
GTEFVADLVTKTPTHLGQGLWLVGSDKQVTRTGVAYVSTANDCDFEGEKVRALVAFAACN
HHHHHHHHHHCCHHHHCCCEEEEECCCCCHHCCEEEEEECCCCCCCCHHHHHHHHHHHHC
NAHQSILSTISKLVFNNEHSKLLNGSAEQILALFKGEEMAAGATEDSNTAIFKIKNAHGL
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCC
HARPGAMLVAEAKKFESTIRVANLDGDGKAVNAKSLMKVIALGVKHGHQLQFSAEGPDAA
CCCCCCEEEHHHHHHHCEEEEEECCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCHHH
QALESIGKAIASGLGEG
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKLSKNDITLSQSATDKFEAIKSIAKSLTDKGLVEQGYVEGMLNRENQNSTFLGNGIAI
CCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCEEC
PHGTTDTRSMVKTTGVAVHHFPEGVEWGEGNTVYVAIGIAAKSDEHLGILKQLTKVLGAD
CCCCCHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC
GVEERLRNAKNEDDIIALLHGNVQLEADFDASLIQLLFPASDMIQMSAVAGGLLKNTGCT
CHHHHHHCCCCCCCEEEEEECCEEEEECCCHHHHHHHCCCHHHHHHHHHHCHHHHCCCCC
GTEFVADLVTKTPTHLGQGLWLVGSDKQVTRTGVAYVSTANDCDFEGEKVRALVAFAACN
HHHHHHHHHHCCHHHHCCCEEEEECCCCCHHCCEEEEEECCCCCCCCHHHHHHHHHHHHC
NAHQSILSTISKLVFNNEHSKLLNGSAEQILALFKGEEMAAGATEDSNTAIFKIKNAHGL
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCC
HARPGAMLVAEAKKFESTIRVANLDGDGKAVNAKSLMKVIALGVKHGHQLQFSAEGPDAA
CCCCCCEEEHHHHHHHCEEEEEECCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCHHH
QALESIGKAIASGLGEG
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]