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The map label for this gene is gloA [H]

Identifier: 156976864

GI number: 156976864

Start: 851327

End: 851713

Strand: Reverse

Name: gloA [H]

Synonym: VIBHAR_05640

Alternate gene names: 156976864

Gene position: 851713-851327 (Counterclockwise)

Preceding gene: 156976871

Following gene: 156976863

Centisome position: 38.64

GC content: 45.22

Gene sequence:

>387_bases
ATGAAATTTCTTCACACTATGATCCGCGTGGCTGACTTAGATAAGTCCATTGAGTTTTACACCAAAGTTCTTGGTATGAG
CGAGCTTGATCGCTTCGAAAACACAGAATACCGCTACACGCTTGTGTTTATTGGTAACGCCGACCAACCAGGCAGCGCGA
CGATTGAACTGACTTACAACTGGGACACATACAGTTATGACCTAGGTAACGCGTTCGGTCACATGGCATTAGGTTGTGAA
GATATTTACGCTGCATGCGACAAAATCAAAGCACTTGGTGGCAACGTCACTCGTGAACCCGGTCCAATGAAAGGCGGTGA
AACTCGCATCGCATTCATTAAAGACCCTGACGGTTACCAAATCGAATTGATTCAAACCAGCAAATAA

Upstream 100 bases:

>100_bases
TTTCATACTTTACCTTTATCTTTAGCTTCAACAAGGTTGCTAGTTGAGATGACTCTGTCACTCCAACGATAGCTAACAAG
CCGAGATAAGAAGGTTAACT

Downstream 100 bases:

>100_bases
CGAGGTTAGTCATGAAAGATACATTGTTTCAACCTGTACAACTTGGTTCTCTGACCCTTCAGAACCGCATCGTTATGCCG
CCAATGACTCGCTCTCGTGC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MKFLHTMIRVADLDKSIEFYTKVLGMSELDRFENTEYRYTLVFIGNADQPGSATIELTYNWDTYSYDLGNAFGHMALGCE
DIYAACDKIKALGGNVTREPGPMKGGETRIAFIKDPDGYQIELIQTSK

Sequences:

>Translated_128_residues
MKFLHTMIRVADLDKSIEFYTKVLGMSELDRFENTEYRYTLVFIGNADQPGSATIELTYNWDTYSYDLGNAFGHMALGCE
DIYAACDKIKALGGNVTREPGPMKGGETRIAFIKDPDGYQIELIQTSK
>Mature_128_residues
MKFLHTMIRVADLDKSIEFYTKVLGMSELDRFENTEYRYTLVFIGNADQPGSATIELTYNWDTYSYDLGNAFGHMALGCE
DIYAACDKIKALGGNVTREPGPMKGGETRIAFIKDPDGYQIELIQTSK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=150, Percent_Identity=36, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=58.4, Blast_Score=158, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.1294964028777, Blast_Score=88, Evalue=4e-19,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=41.2162162162162, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14423; Mature: 14423

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHTMIRVADLDKSIEFYTKVLGMSELDRFENTEYRYTLVFIGNADQPGSATIELTYN
CHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WDTYSYDLGNAFGHMALGCEDIYAACDKIKALGGNVTREPGPMKGGETRIAFIKDPDGYQ
CCCEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIQTSK
EEEEEECC
>Mature Secondary Structure
MKFLHTMIRVADLDKSIEFYTKVLGMSELDRFENTEYRYTLVFIGNADQPGSATIELTYN
CHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WDTYSYDLGNAFGHMALGCEDIYAACDKIKALGGNVTREPGPMKGGETRIAFIKDPDGYQ
CCCEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIQTSK
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]