Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 156976863

GI number: 156976863

Start: 850215

End: 851315

Strand: Reverse

Name: nemA [H]

Synonym: VIBHAR_05639

Alternate gene names: 156976863

Gene position: 851315-850215 (Counterclockwise)

Preceding gene: 156976864

Following gene: 156976861

Centisome position: 38.63

GC content: 48.05

Gene sequence:

>1101_bases
ATGAAAGATACATTGTTTCAACCTGTACAACTTGGTTCTCTGACCCTTCAGAACCGCATCGTTATGCCGCCAATGACTCG
CTCTCGTGCAAGCCAACCTGGTAATGTCGCAAACGACATGATGGCTGAATACTATGCACAGCGCGCATCAGCGGGTTTGA
TTGTTGCAGAAGGCACACAGATTTCACCTATGGGTCAAGGCTACGCTTGGACACCGGGTATTTACAGCCAAGAGCAAATC
GCAGGCTGGAAAAAAGTCACTGACGCAGTACACGCAAAAGGCGGTGCGATCTTCGCCCAACTTTGGCACGTAGGCCGTGT
GACTCACCCTGACAACATCGGTGGCGAACAACCAATTTCTTCTTCTGCACTAAAAGCAGAAAACGTGAAAGTGTTTATTG
ATAACGGCACAGATGAGCCTGGTTTTGTTGATGTGGTTGAACCACGTGAGATGACGAAAGAAGACATCAAACTGGTTATC
GAAGAGTACCGTCAAGCTGCATTGAATGCGATTGAAGCGGGCTTTGATGGCATCGAACTGCACGCAGCAAACGGATATCT
TGTAAACCAGTTCATTGATTCTGAAGCAAACAACCGCACAGATGAATACGGTGGTTCGGTTGAAAATCGTTTACGTTTCT
TGGGTGAAGTGGTTGAAGCGATGACTCAAGCGATTGGTGCTGATCGTGTTGGTGTTCGTCTGGCTCCGTTTACGTCACTA
AATGGTACTGTAGATAAAACACCTGTAGAAACTTACACGGCTGCTGCGGCACTGCTTGATAAGCTAAATGTCGTTTACAT
GCACATTGCAGAAGTAGACTGGGATGACGCACCAGATACGCCAGCTGAGTTCAAAACAGCCGTGCGTGAAGCATACAAGG
GCACCATCATTTACGCTGGTCGCTACAATACAGAGAAAGCAGCTCAAGCCCTAGAAAGTGGTTTGGCAGATATGGTTGGC
TTTGGTCGTCCGTTCATCGCGAACCCTGACCTACCGAACCGTATCAAGAACGATTACCCGCTAGCAGCTCACGATCCTGC
GACGCTGTTTGGTGGTAATGAGAAAGGTCTGTTGGATTACCCGACTTACCAAGCAAACTAA

Upstream 100 bases:

>100_bases
CCGGTCCAATGAAAGGCGGTGAAACTCGCATCGCATTCATTAAAGACCCTGACGGTTACCAAATCGAATTGATTCAAACC
AGCAAATAACGAGGTTAGTC

Downstream 100 bases:

>100_bases
GTCGGTGGGCTGGCTTAGTTCAACCTACTTATTTGAATAGACAAAAACCCCCGCAGATGCGCTTGAGTACATCTGCGGGG
TTTGTTTTTTAGCGCTCAGA

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKDTLFQPVQLGSLTLQNRIVMPPMTRSRASQPGNVANDMMAEYYAQRASAGLIVAEGTQISPMGQGYAWTPGIYSQEQI
AGWKKVTDAVHAKGGAIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKLVI
EEYRQAALNAIEAGFDGIELHAANGYLVNQFIDSEANNRTDEYGGSVENRLRFLGEVVEAMTQAIGADRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLDKLNVVYMHIAEVDWDDAPDTPAEFKTAVREAYKGTIIYAGRYNTEKAAQALESGLADMVG
FGRPFIANPDLPNRIKNDYPLAAHDPATLFGGNEKGLLDYPTYQAN

Sequences:

>Translated_366_residues
MKDTLFQPVQLGSLTLQNRIVMPPMTRSRASQPGNVANDMMAEYYAQRASAGLIVAEGTQISPMGQGYAWTPGIYSQEQI
AGWKKVTDAVHAKGGAIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKLVI
EEYRQAALNAIEAGFDGIELHAANGYLVNQFIDSEANNRTDEYGGSVENRLRFLGEVVEAMTQAIGADRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLDKLNVVYMHIAEVDWDDAPDTPAEFKTAVREAYKGTIIYAGRYNTEKAAQALESGLADMVG
FGRPFIANPDLPNRIKNDYPLAAHDPATLFGGNEKGLLDYPTYQAN
>Mature_366_residues
MKDTLFQPVQLGSLTLQNRIVMPPMTRSRASQPGNVANDMMAEYYAQRASAGLIVAEGTQISPMGQGYAWTPGIYSQEQI
AGWKKVTDAVHAKGGAIFAQLWHVGRVTHPDNIGGEQPISSSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKLVI
EEYRQAALNAIEAGFDGIELHAANGYLVNQFIDSEANNRTDEYGGSVENRLRFLGEVVEAMTQAIGADRVGVRLAPFTSL
NGTVDKTPVETYTAAAALLDKLNVVYMHIAEVDWDDAPDTPAEFKTAVREAYKGTIIYAGRYNTEKAAQALESGLADMVG
FGRPFIANPDLPNRIKNDYPLAAHDPATLFGGNEKGLLDYPTYQAN

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=43.6813186813187, Blast_Score=307, Evalue=6e-85,
Organism=Escherichia coli, GI1789463, Length=366, Percent_Identity=29.5081967213115, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17565138, Length=308, Percent_Identity=31.4935064935065, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=300, Percent_Identity=30.3333333333333, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=204, Percent_Identity=33.8235294117647, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=274, Percent_Identity=30.2919708029197, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=211, Percent_Identity=30.3317535545024, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=365, Percent_Identity=25.7534246575342, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=352, Percent_Identity=25.2840909090909, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=38.7012987012987, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=37.4025974025974, Blast_Score=243, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39832; Mature: 39832

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTLFQPVQLGSLTLQNRIVMPPMTRSRASQPGNVANDMMAEYYAQRASAGLIVAEGTQ
CCCCCCCCHHHCCEEECCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCE
ISPMGQGYAWTPGIYSQEQIAGWKKVTDAVHAKGGAIFAQLWHVGRVTHPDNIGGEQPIS
ECCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKLVIEEYRQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLVNQFIDSEANNRTDEYGGSVENRLRFLGEVVEAMTQAIGADRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDKTPVETYTAAAALLDKLNVVYMHIAEVDWDDAPDTPAEFKTAVREAYKGTIIYAG
CCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEC
RYNTEKAAQALESGLADMVGFGRPFIANPDLPNRIKNDYPLAAHDPATLFGGNEKGLLDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCEEC
PTYQAN
CCCCCC
>Mature Secondary Structure
MKDTLFQPVQLGSLTLQNRIVMPPMTRSRASQPGNVANDMMAEYYAQRASAGLIVAEGTQ
CCCCCCCCHHHCCEEECCEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCE
ISPMGQGYAWTPGIYSQEQIAGWKKVTDAVHAKGGAIFAQLWHVGRVTHPDNIGGEQPIS
ECCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SSALKAENVKVFIDNGTDEPGFVDVVEPREMTKEDIKLVIEEYRQAALNAIEAGFDGIEL
CCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLVNQFIDSEANNRTDEYGGSVENRLRFLGEVVEAMTQAIGADRVGVRLAPFTSL
EECCCEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
NGTVDKTPVETYTAAAALLDKLNVVYMHIAEVDWDDAPDTPAEFKTAVREAYKGTIIYAG
CCCCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEC
RYNTEKAAQALESGLADMVGFGRPFIANPDLPNRIKNDYPLAAHDPATLFGGNEKGLLDY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCEEC
PTYQAN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]