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The map label for this gene is rstB [H]

Identifier: 156976558

GI number: 156976558

Start: 564430

End: 565752

Strand: Direct

Name: rstB [H]

Synonym: VIBHAR_05332

Alternate gene names: 156976558

Gene position: 564430-565752 (Clockwise)

Preceding gene: 156976557

Following gene: 156976560

Centisome position: 25.61

GC content: 46.86

Gene sequence:

>1323_bases
ATGTTCTCTCTGTTTACTCGGCTCTATGTTGGATTGGTAACGGGCCTTGCAGTGACTGTCGCCTTGTTTTTTACTATGGG
TGACGAGTTTATGCGCAAAACTGAAGTTGGCATCTTCTTGAGTGATGGTACGTATTTTGTCGATCAGTATACTGAGCAAC
GTGGGACGCCTAACTCACTCTACAAGCAGTTAACGAGAACAGGCAAACAGGATTTTTTCATCTTCGACCTGACTCTTCTT
AATAACTGGGACGGTTCGCCTCCTTGTTTGGACTGCGAATTGCTTTATCGTATGCAAGGTGTGCCCGTGTACTTGATCGA
TGGCAATATCTACGCTGCGGTTTTTCCACTCCCAAACTCATCAGAAAGTCTGGTATTTAAAGAAAAGCGAGATTTTTTTA
GTCCAGATGTTGATTGGGATGAAGACTCAGAAAGCATCTTTGTGTTCAGCTTGTTATTCACCATAATATGCAGCTTGGGT
GTCTTGGTTTATGTTCCGGTGCGTAGACTTGATCGCCAGATTCAACAATTGACGTCAGCACAGCAGCAATTTGGCTCCGG
TGAGCTCAGCACTCGTGTGGATTTAAAGCACTTCTCTTCTCCGGTCCGAGAGCTTGCTACTGGCTTCAACAACATGTCGG
AAGACATTGAAAGGCAAGTGCGCCAAACACACATTTTCACTCTGGCTATCCCTCATGAAGTTCGAACGCCACTGAGTCGA
ATTCAGCTAGCTACCGACTTGGCAAGGCGTACTAAAGGTGAAATGCAAGCGGAACTGCACGATGACATTGAAAGATATGT
GGATGTGGTGACGGATTTAACGTCTGACATCGTAATGCTTTCCCGCTTGAGCGTGAATAACTATTCGTTAGCCGATTCTG
TTGAAACCATTGAACTGCCGCTTTGGTGTAAAAGCCGCATGAATTATCGTGGGTTAGTGTCAGCGAAGCTTACTGTTGAG
AATGCTTTGGCTTACCAGTCCATCCACCTGCAACCAACGTTGGGTAATTTGGTTCTTGATAACCTACTGCAGAATGCAAA
ACGTTATGGCGAGTCTTGTGTGGAAGTGACGATCCGGTCGGTAGAGCACTTCTGGATCGTCGATATTGAAGATGATGGCC
CAGGGGTTCCTAAAGAGATGCGTGAAGAGGTATTCATTCCGTTTTCTCGTATAGATAAAAGCCGTAACGCCGATGTCAAA
GGTTTTGGCTTGGGACTCGCCATTGCGTCTAGTGCTGCGAGGCAACTAGGCTGGGAGCTGTCGGTCGGCGAGAGCCACTT
AGGGGGCGCGTTGTTTACCGTGCTTATTCCTGCCAGCGATTAG

Upstream 100 bases:

>100_bases
AATCTCTTCTTTGAGGAAAAAATTCTCAAAAGCCCTTATTCACACCGCTACCATTAAGACCGTGCGTAATCGTGGATACA
TGTTAACGGGGAAATAGCGG

Downstream 100 bases:

>100_bases
GGTAGGCACGACGGTAGTCTGGCTCTTTAATGCCTTCATACAAATCTTCACAAGCTTGCAGCGGGCCTGCAACAGTTCGG
TACGGCAAGACTCCAGCCCA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MFSLFTRLYVGLVTGLAVTVALFFTMGDEFMRKTEVGIFLSDGTYFVDQYTEQRGTPNSLYKQLTRTGKQDFFIFDLTLL
NNWDGSPPCLDCELLYRMQGVPVYLIDGNIYAAVFPLPNSSESLVFKEKRDFFSPDVDWDEDSESIFVFSLLFTIICSLG
VLVYVPVRRLDRQIQQLTSAQQQFGSGELSTRVDLKHFSSPVRELATGFNNMSEDIERQVRQTHIFTLAIPHEVRTPLSR
IQLATDLARRTKGEMQAELHDDIERYVDVVTDLTSDIVMLSRLSVNNYSLADSVETIELPLWCKSRMNYRGLVSAKLTVE
NALAYQSIHLQPTLGNLVLDNLLQNAKRYGESCVEVTIRSVEHFWIVDIEDDGPGVPKEMREEVFIPFSRIDKSRNADVK
GFGLGLAIASSAARQLGWELSVGESHLGGALFTVLIPASD

Sequences:

>Translated_440_residues
MFSLFTRLYVGLVTGLAVTVALFFTMGDEFMRKTEVGIFLSDGTYFVDQYTEQRGTPNSLYKQLTRTGKQDFFIFDLTLL
NNWDGSPPCLDCELLYRMQGVPVYLIDGNIYAAVFPLPNSSESLVFKEKRDFFSPDVDWDEDSESIFVFSLLFTIICSLG
VLVYVPVRRLDRQIQQLTSAQQQFGSGELSTRVDLKHFSSPVRELATGFNNMSEDIERQVRQTHIFTLAIPHEVRTPLSR
IQLATDLARRTKGEMQAELHDDIERYVDVVTDLTSDIVMLSRLSVNNYSLADSVETIELPLWCKSRMNYRGLVSAKLTVE
NALAYQSIHLQPTLGNLVLDNLLQNAKRYGESCVEVTIRSVEHFWIVDIEDDGPGVPKEMREEVFIPFSRIDKSRNADVK
GFGLGLAIASSAARQLGWELSVGESHLGGALFTVLIPASD
>Mature_440_residues
MFSLFTRLYVGLVTGLAVTVALFFTMGDEFMRKTEVGIFLSDGTYFVDQYTEQRGTPNSLYKQLTRTGKQDFFIFDLTLL
NNWDGSPPCLDCELLYRMQGVPVYLIDGNIYAAVFPLPNSSESLVFKEKRDFFSPDVDWDEDSESIFVFSLLFTIICSLG
VLVYVPVRRLDRQIQQLTSAQQQFGSGELSTRVDLKHFSSPVRELATGFNNMSEDIERQVRQTHIFTLAIPHEVRTPLSR
IQLATDLARRTKGEMQAELHDDIERYVDVVTDLTSDIVMLSRLSVNNYSLADSVETIELPLWCKSRMNYRGLVSAKLTVE
NALAYQSIHLQPTLGNLVLDNLLQNAKRYGESCVEVTIRSVEHFWIVDIEDDGPGVPKEMREEVFIPFSRIDKSRNADVK
GFGLGLAIASSAARQLGWELSVGESHLGGALFTVLIPASD

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=290, Percent_Identity=32.0689655172414, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790346, Length=269, Percent_Identity=31.2267657992565, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789808, Length=304, Percent_Identity=25.3289473684211, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=27.6666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=26.2987012987013, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=30.4147465437788, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=99, Percent_Identity=41.4141414141414, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790551, Length=275, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081816, Length=254, Percent_Identity=26.7716535433071, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=23.8095238095238, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49638; Mature: 49638

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLFTRLYVGLVTGLAVTVALFFTMGDEFMRKTEVGIFLSDGTYFVDQYTEQRGTPNSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCEEEEECCEEEEHHHHHHCCCHHHH
YKQLTRTGKQDFFIFDLTLLNNWDGSPPCLDCELLYRMQGVPVYLIDGNIYAAVFPLPNS
HHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCCEEEEEEECCCC
SESLVFKEKRDFFSPDVDWDEDSESIFVFSLLFTIICSLGVLVYVPVRRLDRQIQQLTSA
CCCEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQQFGSGELSTRVDLKHFSSPVRELATGFNNMSEDIERQVRQTHIFTLAIPHEVRTPLSR
HHHCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCHHHHCHHHH
IQLATDLARRTKGEMQAELHDDIERYVDVVTDLTSDIVMLSRLSVNNYSLADSVETIELP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
LWCKSRMNYRGLVSAKLTVENALAYQSIHLQPTLGNLVLDNLLQNAKRYGESCVEVTIRS
HHHHCCCCCCEEEEEEEEHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VEHFWIVDIEDDGPGVPKEMREEVFIPFSRIDKSRNADVKGFGLGLAIASSAARQLGWEL
CCEEEEEEEECCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCEE
SVGESHLGGALFTVLIPASD
ECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MFSLFTRLYVGLVTGLAVTVALFFTMGDEFMRKTEVGIFLSDGTYFVDQYTEQRGTPNSL
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCEEEEECCEEEEHHHHHHCCCHHHH
YKQLTRTGKQDFFIFDLTLLNNWDGSPPCLDCELLYRMQGVPVYLIDGNIYAAVFPLPNS
HHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCCEEEEEEECCCC
SESLVFKEKRDFFSPDVDWDEDSESIFVFSLLFTIICSLGVLVYVPVRRLDRQIQQLTSA
CCCEEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQQFGSGELSTRVDLKHFSSPVRELATGFNNMSEDIERQVRQTHIFTLAIPHEVRTPLSR
HHHCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCHHHHCHHHH
IQLATDLARRTKGEMQAELHDDIERYVDVVTDLTSDIVMLSRLSVNNYSLADSVETIELP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
LWCKSRMNYRGLVSAKLTVENALAYQSIHLQPTLGNLVLDNLLQNAKRYGESCVEVTIRS
HHHHCCCCCCEEEEEEEEHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VEHFWIVDIEDDGPGVPKEMREEVFIPFSRIDKSRNADVKGFGLGLAIASSAARQLGWEL
CCEEEEEEEECCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCEE
SVGESHLGGALFTVLIPASD
ECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]