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The map label for this gene is rstA [H]

Identifier: 156976520

GI number: 156976520

Start: 531918

End: 532631

Strand: Reverse

Name: rstA [H]

Synonym: VIBHAR_05293

Alternate gene names: 156976520

Gene position: 532631-531918 (Counterclockwise)

Preceding gene: 156976521

Following gene: 156976519

Centisome position: 24.17

GC content: 47.2

Gene sequence:

>714_bases
ATGAACGTACAAGACATTAAGCAATCACTTATCATCGTGGAAGACGATCCAAAATTGCAGGCTATGCTAAGCGAATACTT
TGAAGGACAAGGCTTCGAGGTTAGCTGCATAGACAATGGAACGGACGCTCCGGATGCGATTCTTGAAAGCCAGCCAGATC
TCGTTCTTTTAGATTTGATGTTGCCGGGGCAAGATGGATTGAGCGTTTGCCGCCAGATCCGCGAATGCTATAAAGGCAAA
GTGCTGATGCTTACTGCCAGTGATGATGATTTTGACCATGTTGCTGCGCTTGAAATCGGTGCCGATGATTTCGTTACCAA
ACCGATTAAACCTCGCGTGTTACTCGCTCGCATTCGAATGCTATTACGCCGTAAAGAAGCATCTGCAACAGATTCAGACA
CGTGTTTAGAAGTTGGCGTCCTGTGCCTTAATCGCCTGCGTAAAACATGCGCTCTAAACCAAGAACCCATTCCTCTCACC
GACGGTGAATTTGATCTCCTTTGGGTACTCGCAAACCATCCTGAACAAACCCTTTCTCGCGAGTGGCTCACCAAAAAATT
ACGTGGCATCGAATACGACGGCACCGACCGAACTATTGATAACCGTGTCGTAACGCTGCGTAAAAAGTTAGGCGACAGCA
CCACACCACCACAGAAAATCATTACTGTGCGCGGTAAAGGTTACTTGTTTATGCCTGATGCTTGGAACGCGTAA

Upstream 100 bases:

>100_bases
ACTCTCGTCTATAAATCCCCTCTGACTCTTTACGTTTATTGAAATCCCTGACAAATTTGTTTAAGTTTCAGGGATAATTC
CTACAAGATGCATCACTGCT

Downstream 100 bases:

>100_bases
TGAAGCGCATTTATCTGGAAAGCTTTTTAGGTCTGATCATTTTATTTGTAGCCAGTCTTGTTGGCTATGAAGTCATTGTT
TATCAACTCAATACGGACTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNVQDIKQSLIIVEDDPKLQAMLSEYFEGQGFEVSCIDNGTDAPDAILESQPDLVLLDLMLPGQDGLSVCRQIRECYKGK
VLMLTASDDDFDHVAALEIGADDFVTKPIKPRVLLARIRMLLRRKEASATDSDTCLEVGVLCLNRLRKTCALNQEPIPLT
DGEFDLLWVLANHPEQTLSREWLTKKLRGIEYDGTDRTIDNRVVTLRKKLGDSTTPPQKIITVRGKGYLFMPDAWNA

Sequences:

>Translated_237_residues
MNVQDIKQSLIIVEDDPKLQAMLSEYFEGQGFEVSCIDNGTDAPDAILESQPDLVLLDLMLPGQDGLSVCRQIRECYKGK
VLMLTASDDDFDHVAALEIGADDFVTKPIKPRVLLARIRMLLRRKEASATDSDTCLEVGVLCLNRLRKTCALNQEPIPLT
DGEFDLLWVLANHPEQTLSREWLTKKLRGIEYDGTDRTIDNRVVTLRKKLGDSTTPPQKIITVRGKGYLFMPDAWNA
>Mature_237_residues
MNVQDIKQSLIIVEDDPKLQAMLSEYFEGQGFEVSCIDNGTDAPDAILESQPDLVLLDLMLPGQDGLSVCRQIRECYKGK
VLMLTASDDDFDHVAALEIGADDFVTKPIKPRVLLARIRMLLRRKEASATDSDTCLEVGVLCLNRLRKTCALNQEPIPLT
DGEFDLLWVLANHPEQTLSREWLTKKLRGIEYDGTDRTIDNRVVTLRKKLGDSTTPPQKIITVRGKGYLFMPDAWNA

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=39.662447257384, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=38.6266094420601, Blast_Score=173, Evalue=9e-45,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=36.2445414847162, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=34.4827586206897, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=37.4449339207048, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=32.4675324675325, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=30.9417040358744, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=34.5291479820628, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=34.8017621145374, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=31.8777292576419, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=32.8828828828829, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=31.5315315315315, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788550, Length=101, Percent_Identity=35.6435643564356, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=99, Percent_Identity=31.3131313131313, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26619; Mature: 26619

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQDIKQSLIIVEDDPKLQAMLSEYFEGQGFEVSCIDNGTDAPDAILESQPDLVLLDLM
CCHHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEEE
LPGQDGLSVCRQIRECYKGKVLMLTASDDDFDHVAALEIGADDFVTKPIKPRVLLARIRM
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHH
LLRRKEASATDSDTCLEVGVLCLNRLRKTCALNQEPIPLTDGEFDLLWVLANHPEQTLSR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
EWLTKKLRGIEYDGTDRTIDNRVVTLRKKLGDSTTPPQKIITVRGKGYLFMPDAWNA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MNVQDIKQSLIIVEDDPKLQAMLSEYFEGQGFEVSCIDNGTDAPDAILESQPDLVLLDLM
CCHHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEEE
LPGQDGLSVCRQIRECYKGKVLMLTASDDDFDHVAALEIGADDFVTKPIKPRVLLARIRM
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHH
LLRRKEASATDSDTCLEVGVLCLNRLRKTCALNQEPIPLTDGEFDLLWVLANHPEQTLSR
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
EWLTKKLRGIEYDGTDRTIDNRVVTLRKKLGDSTTPPQKIITVRGKGYLFMPDAWNA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]