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The map label for this gene is rstB [H]

Identifier: 156976519

GI number: 156976519

Start: 530632

End: 531918

Strand: Reverse

Name: rstB [H]

Synonym: VIBHAR_05292

Alternate gene names: 156976519

Gene position: 531918-530632 (Counterclockwise)

Preceding gene: 156976520

Following gene: 156976518

Centisome position: 24.13

GC content: 44.76

Gene sequence:

>1287_bases
ATGAAGCGCATTTATCTGGAAAGCTTTTTAGGTCTGATCATTTTATTTGTAGCCAGTCTTGTTGGCTATGAAGTCATTGT
TTATCAACTCAATACGGACTACGACTACCTACTCGAAGAGCATGAAGCTCAAGCTTTCCATGAGCTGATTTATCCGGTGT
ATCAAGAGAAAGGTGAAGATTACACCGTTCAGCAGTTAGAGAAATTCGCAACATCGAGTCACATGTTGTTAATTGCTCAG
GAAATGACGAGTCTTCCTCCTGAGATACAACAAGCATTCGATGAAGATCCATCTGTGAATACGGCTTTTGATGATGAGCG
AGACTTGTGGTTTCGTTTTGAACCCGGTTCGCCTATTTTCAAGTTAAGCCAAAACCCCAACAGCCCTATCATTCAAGCCA
TCAACTTTGATGACAACATCATTTGGCTGTTCTTTCTTGCCGGATTTGCGCTTTACTGTGTCTTACTGATTTGGTTCTTA
AGCCGACGCGTTCGCGCTTTAGAAAAGGTTACCGTTGATTTTGCCTCTGGTGATCTTAACGCCAGAACGGATACCTCCAG
CGCCAAATCCGTTGGAACTTTGAACAGAAGCTTCAACCATATGGCAGATAAGGTATCTCGACTCATCACCACAAACAAAA
TGCTCACCAATGCAGTAGCACATGAGCTTCGTACTCCAATCTTTCGTCTTCAATGGCAAGCCGATCTACTTGCCGACGCG
GCTTTAAATGAACAACAACAGAAGTACGTCGACAGCATCGTAGAAGATATCGACGACATGGAAAATATGGTGGAAGAACT
CCTCTACTACGCCAAAATGGAGCGACCAAATGCCGAGATTCAAACTTCCTCTGTCGTCTTATCGAGCTTTGCTCAGTCGC
TTTTACCTAAGTGGCAACGTGACACCCAACTTTCCATCTTGATTGACGACACGAGCTCACAACAGGCGATCATTGACGCC
GACCCAAAACTGCTAAAACGTGCTTTAGATAACCTACTTCGCAATGCAATGCGTTATGCGCAAACCCAAATTGTGCTAGA
GATTATGGAGACACCAGAGAAATCTCGTATTCGCGTGCATGACGATGGCTGTGGTGTGGAAGAGAAAGATTGGCCTCATC
TGTTTGATGCGTTTTACAGTGCTGATAAGTCGCGTAACAAGAGCACTAGCGGGTTCGGTTTGGGGCTTGCTATCGTCAAA
CAGATCGTTGAATTGCAGAACGGCGAAGTCTATGTCTCACACAGCCAGTACGGTGGTGCGTGTTTTACAATCTGCTTTCC
CAAATAA

Upstream 100 bases:

>100_bases
CGCTGCGTAAAAAGTTAGGCGACAGCACCACACCACCACAGAAAATCATTACTGTGCGCGGTAAAGGTTACTTGTTTATG
CCTGATGCTTGGAACGCGTA

Downstream 100 bases:

>100_bases
ATTCATAAACTTATCAATACTCCCTTAAACGCCATTACAAAAGGCGACACTTCAATACAATTGGCCGACAGAGCACCACA
ATAAAACAGTGTACATTTAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRIYLESFLGLIILFVASLVGYEVIVYQLNTDYDYLLEEHEAQAFHELIYPVYQEKGEDYTVQQLEKFATSSHMLLIAQ
EMTSLPPEIQQAFDEDPSVNTAFDDERDLWFRFEPGSPIFKLSQNPNSPIIQAINFDDNIIWLFFLAGFALYCVLLIWFL
SRRVRALEKVTVDFASGDLNARTDTSSAKSVGTLNRSFNHMADKVSRLITTNKMLTNAVAHELRTPIFRLQWQADLLADA
ALNEQQQKYVDSIVEDIDDMENMVEELLYYAKMERPNAEIQTSSVVLSSFAQSLLPKWQRDTQLSILIDDTSSQQAIIDA
DPKLLKRALDNLLRNAMRYAQTQIVLEIMETPEKSRIRVHDDGCGVEEKDWPHLFDAFYSADKSRNKSTSGFGLGLAIVK
QIVELQNGEVYVSHSQYGGACFTICFPK

Sequences:

>Translated_428_residues
MKRIYLESFLGLIILFVASLVGYEVIVYQLNTDYDYLLEEHEAQAFHELIYPVYQEKGEDYTVQQLEKFATSSHMLLIAQ
EMTSLPPEIQQAFDEDPSVNTAFDDERDLWFRFEPGSPIFKLSQNPNSPIIQAINFDDNIIWLFFLAGFALYCVLLIWFL
SRRVRALEKVTVDFASGDLNARTDTSSAKSVGTLNRSFNHMADKVSRLITTNKMLTNAVAHELRTPIFRLQWQADLLADA
ALNEQQQKYVDSIVEDIDDMENMVEELLYYAKMERPNAEIQTSSVVLSSFAQSLLPKWQRDTQLSILIDDTSSQQAIIDA
DPKLLKRALDNLLRNAMRYAQTQIVLEIMETPEKSRIRVHDDGCGVEEKDWPHLFDAFYSADKSRNKSTSGFGLGLAIVK
QIVELQNGEVYVSHSQYGGACFTICFPK
>Mature_428_residues
MKRIYLESFLGLIILFVASLVGYEVIVYQLNTDYDYLLEEHEAQAFHELIYPVYQEKGEDYTVQQLEKFATSSHMLLIAQ
EMTSLPPEIQQAFDEDPSVNTAFDDERDLWFRFEPGSPIFKLSQNPNSPIIQAINFDDNIIWLFFLAGFALYCVLLIWFL
SRRVRALEKVTVDFASGDLNARTDTSSAKSVGTLNRSFNHMADKVSRLITTNKMLTNAVAHELRTPIFRLQWQADLLADA
ALNEQQQKYVDSIVEDIDDMENMVEELLYYAKMERPNAEIQTSSVVLSSFAQSLLPKWQRDTQLSILIDDTSSQQAIIDA
DPKLLKRALDNLLRNAMRYAQTQIVLEIMETPEKSRIRVHDDGCGVEEKDWPHLFDAFYSADKSRNKSTSGFGLGLAIVK
QIVELQNGEVYVSHSQYGGACFTICFPK

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=416, Percent_Identity=28.8461538461538, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1789808, Length=386, Percent_Identity=25.9067357512953, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=28.9377289377289, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=27.2727272727273, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=25.9414225941423, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=27.7272727272727, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790861, Length=209, Percent_Identity=28.2296650717703, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=222, Percent_Identity=28.8288288288288, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=26.6375545851528, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082128, Length=293, Percent_Identity=21.160409556314, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=219, Percent_Identity=26.4840182648402, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=26.6891891891892, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=24.3801652892562, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790551, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=213, Percent_Identity=24.4131455399061, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=220, Percent_Identity=22.7272727272727, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=27.3542600896861, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48940; Mature: 48940

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIYLESFLGLIILFVASLVGYEVIVYQLNTDYDYLLEEHEAQAFHELIYPVYQEKGED
CCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YTVQQLEKFATSSHMLLIAQEMTSLPPEIQQAFDEDPSVNTAFDDERDLWFRFEPGSPIF
CHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEE
KLSQNPNSPIIQAINFDDNIIWLFFLAGFALYCVLLIWFLSRRVRALEKVTVDFASGDLN
EECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
ARTDTSSAKSVGTLNRSFNHMADKVSRLITTNKMLTNAVAHELRTPIFRLQWQADLLADA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHH
ALNEQQQKYVDSIVEDIDDMENMVEELLYYAKMERPNAEIQTSSVVLSSFAQSLLPKWQR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DTQLSILIDDTSSQQAIIDADPKLLKRALDNLLRNAMRYAQTQIVLEIMETPEKSRIRVH
CCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DDGCGVEEKDWPHLFDAFYSADKSRNKSTSGFGLGLAIVKQIVELQNGEVYVSHSQYGGA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCE
CFTICFPK
EEEEEECC
>Mature Secondary Structure
MKRIYLESFLGLIILFVASLVGYEVIVYQLNTDYDYLLEEHEAQAFHELIYPVYQEKGED
CCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YTVQQLEKFATSSHMLLIAQEMTSLPPEIQQAFDEDPSVNTAFDDERDLWFRFEPGSPIF
CHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEE
KLSQNPNSPIIQAINFDDNIIWLFFLAGFALYCVLLIWFLSRRVRALEKVTVDFASGDLN
EECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
ARTDTSSAKSVGTLNRSFNHMADKVSRLITTNKMLTNAVAHELRTPIFRLQWQADLLADA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHH
ALNEQQQKYVDSIVEDIDDMENMVEELLYYAKMERPNAEIQTSSVVLSSFAQSLLPKWQR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DTQLSILIDDTSSQQAIIDADPKLLKRALDNLLRNAMRYAQTQIVLEIMETPEKSRIRVH
CCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DDGCGVEEKDWPHLFDAFYSADKSRNKSTSGFGLGLAIVKQIVELQNGEVYVSHSQYGGA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCE
CFTICFPK
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]