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The map label for this gene is 156976485

Identifier: 156976485

GI number: 156976485

Start: 506643

End: 507758

Strand: Reverse

Name: 156976485

Synonym: VIBHAR_05258

Alternate gene names: NA

Gene position: 507758-506643 (Counterclockwise)

Preceding gene: 156976488

Following gene: 156976482

Centisome position: 23.04

GC content: 45.25

Gene sequence:

>1116_bases
ATGGTGAGAATTACACGTAATAATGACCTCGTATTGAAACGTTACGAGGGCAATTCAATGAAAGGTTTTAGCAAAAAAGT
TCTTCTAGCAGCATCTGTTGCAATGGCAGTTGGCAGCATCTCTACCGCAGCAAATGCGTGTTCACGTTTGGTTTGGGAGA
CTCAAGACCACGGTGTATTTGTCTCTCGTACCATGGATTGGATGGAAGCCAATCAGCCAACCATTGATGTTCGTCACGCA
GGCCAAACCTACCGCGGCTACGATAAAGATGATGCGCTTACGTGCACATCGAAATACGCTTCTATCGGTGTAAGTATTTA
CGGCGTGGGCATTATTGATGGCTTTAACGAGAAAGGCTTTGCGGCGAATGCCCTGTTCTTGGATGAAGAAAACCCAGGGG
CACCACAAGCTGGCAAACAACAAATTGAAAACTCGCGTTTTGTCGCCTATTTGATTGATAGCTTTGCATCCGTTGATGAA
GCATTGAAGAATCTAGCTTCAATCCAAATCCAACAGTTCAGCCACAATGGCATCGAAATGAAGGGACATTATTCATTAGA
AGACGCAAGTGGTGACTCTGCTGTGTTGGAATACATCGACGGCGCATGGCAAGTTCATCATGGCAAACAATATGACGTGA
TGACTAACAGCCCAGAGTACGCGCAACACTTGAAAAACTGGCAAGAAGCGCAACCAAAAGCGAAGAGTGATGTGAATGGT
GAGTTCCCGATCCCAGGTAACATTAATTCTGCTCAACGCTTTATCTGGAACAGCTACATGAAGGACCAACTTAAAGAGCC
TAGCAGCTACACCAACGGCATTGCGAAACTGGATAGTGTGACCTATAAAATCCCATTGGATGCGGCAAACCGCCCAGTGA
ATGGCGAAATGCGTGGCTACGCAACCATCTATGGTTTGGTGTACAACCTAGACCAAAAAGTAATGAACGTGCGTTACCAG
TATGACGACAGTTACACGCAGTACTCAGTGGACTTTAATAAGTTGAATGACGGCCATAACTACACCATCAAAGCCGATTT
ACCGGATCTGTTTGGTGACATTTCATCTCGCTTAGAAAAAGGCGATGGCGTAATGGGTCAACACTTAGTGAAATAA

Upstream 100 bases:

>100_bases
ACTGGCTCTGGGCATTGGAAGCTCCTCTAACAATAAACCCGTTTATCTTAGCTCAACCGATATCCTTTTGTTGCAAAGTT
TGCAACATAACTGAGATTAA

Downstream 100 bases:

>100_bases
CTCAGATTACGTAAAAAACAGAACTTGAAAGGTGTGTGGACAAAAGCACCATTCAAAAAAGCGCCGACATGTTCGGCGCT
TTTCCTTTCTATTCGTTTTG

Product: penicillin acylase

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVRITRNNDLVLKRYEGNSMKGFSKKVLLAASVAMAVGSISTAANACSRLVWETQDHGVFVSRTMDWMEANQPTIDVRHA
GQTYRGYDKDDALTCTSKYASIGVSIYGVGIIDGFNEKGFAANALFLDEENPGAPQAGKQQIENSRFVAYLIDSFASVDE
ALKNLASIQIQQFSHNGIEMKGHYSLEDASGDSAVLEYIDGAWQVHHGKQYDVMTNSPEYAQHLKNWQEAQPKAKSDVNG
EFPIPGNINSAQRFIWNSYMKDQLKEPSSYTNGIAKLDSVTYKIPLDAANRPVNGEMRGYATIYGLVYNLDQKVMNVRYQ
YDDSYTQYSVDFNKLNDGHNYTIKADLPDLFGDISSRLEKGDGVMGQHLVK

Sequences:

>Translated_371_residues
MVRITRNNDLVLKRYEGNSMKGFSKKVLLAASVAMAVGSISTAANACSRLVWETQDHGVFVSRTMDWMEANQPTIDVRHA
GQTYRGYDKDDALTCTSKYASIGVSIYGVGIIDGFNEKGFAANALFLDEENPGAPQAGKQQIENSRFVAYLIDSFASVDE
ALKNLASIQIQQFSHNGIEMKGHYSLEDASGDSAVLEYIDGAWQVHHGKQYDVMTNSPEYAQHLKNWQEAQPKAKSDVNG
EFPIPGNINSAQRFIWNSYMKDQLKEPSSYTNGIAKLDSVTYKIPLDAANRPVNGEMRGYATIYGLVYNLDQKVMNVRYQ
YDDSYTQYSVDFNKLNDGHNYTIKADLPDLFGDISSRLEKGDGVMGQHLVK
>Mature_371_residues
MVRITRNNDLVLKRYEGNSMKGFSKKVLLAASVAMAVGSISTAANACSRLVWETQDHGVFVSRTMDWMEANQPTIDVRHA
GQTYRGYDKDDALTCTSKYASIGVSIYGVGIIDGFNEKGFAANALFLDEENPGAPQAGKQQIENSRFVAYLIDSFASVDE
ALKNLASIQIQQFSHNGIEMKGHYSLEDASGDSAVLEYIDGAWQVHHGKQYDVMTNSPEYAQHLKNWQEAQPKAKSDVNG
EFPIPGNINSAQRFIWNSYMKDQLKEPSSYTNGIAKLDSVTYKIPLDAANRPVNGEMRGYATIYGLVYNLDQKVMNVRYQ
YDDSYTQYSVDFNKLNDGHNYTIKADLPDLFGDISSRLEKGDGVMGQHLVK

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 41346; Mature: 41346

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRITRNNDLVLKRYEGNSMKGFSKKVLLAASVAMAVGSISTAANACSRLVWETQDHGVF
CEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEE
VSRTMDWMEANQPTIDVRHAGQTYRGYDKDDALTCTSKYASIGVSIYGVGIIDGFNEKGF
EEECHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHCCEEEEEEEEEECCCCCCC
AANALFLDEENPGAPQAGKQQIENSRFVAYLIDSFASVDEALKNLASIQIQQFSHNGIEM
EEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEE
KGHYSLEDASGDSAVLEYIDGAWQVHHGKQYDVMTNSPEYAQHLKNWQEAQPKAKSDVNG
ECCEEECCCCCCHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
EFPIPGNINSAQRFIWNSYMKDQLKEPSSYTNGIAKLDSVTYKIPLDAANRPVNGEMRGY
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHH
ATIYGLVYNLDQKVMNVRYQYDDSYTQYSVDFNKLNDGHNYTIKADLPDLFGDISSRLEK
HEEHHHHHHHCHHHHEEEEEECCCEEEEEEEHHHCCCCCCEEEEECCHHHHHHHHHHHHH
GDGVMGQHLVK
CCCCCHHHHCC
>Mature Secondary Structure
MVRITRNNDLVLKRYEGNSMKGFSKKVLLAASVAMAVGSISTAANACSRLVWETQDHGVF
CEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEE
VSRTMDWMEANQPTIDVRHAGQTYRGYDKDDALTCTSKYASIGVSIYGVGIIDGFNEKGF
EEECHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHCCEEEEEEEEEECCCCCCC
AANALFLDEENPGAPQAGKQQIENSRFVAYLIDSFASVDEALKNLASIQIQQFSHNGIEM
EEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEE
KGHYSLEDASGDSAVLEYIDGAWQVHHGKQYDVMTNSPEYAQHLKNWQEAQPKAKSDVNG
ECCEEECCCCCCHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCC
EFPIPGNINSAQRFIWNSYMKDQLKEPSSYTNGIAKLDSVTYKIPLDAANRPVNGEMRGY
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHH
ATIYGLVYNLDQKVMNVRYQYDDSYTQYSVDFNKLNDGHNYTIKADLPDLFGDISSRLEK
HEEHHHHHHHCHHHHEEEEEECCCEEEEEEEHHHCCCCCCEEEEECCHHHHHHHHHHHHH
GDGVMGQHLVK
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]