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The map label for this gene is frwB [H]

Identifier: 156976414

GI number: 156976414

Start: 444006

End: 444305

Strand: Reverse

Name: frwB [H]

Synonym: VIBHAR_05187

Alternate gene names: 156976414

Gene position: 444305-444006 (Counterclockwise)

Preceding gene: 156976417

Following gene: 156976413

Centisome position: 20.16

GC content: 46.67

Gene sequence:

>300_bases
ATGAAAATCGTAGCGGTGACAGCGTGCCCTACAGGCATCGCTCATACTTATATGGCGGCAGATGCGCTAATGAAAGCTGC
GCCTAAGCATAACGTACAAATTAAGGTCGAAACGCAAGGTGCGATGGGTATTGAGAATCAGCTCACTCCCCACGACATTG
CACAAGCGGATCGTGTCTTAATTGTGTCCGATATCGAGATTGAACAGCCAGCTCGCTTTGAAGGCACCAACAAAGTCCAA
ATCCCAATTGAAGACGTGCTACTTAATGTTGATAAGGTGTTCCTTGTACACTATCGATGA

Upstream 100 bases:

>100_bases
GAAATTTAAACTGTGATAGAGCTCTTGCGATTATCTGCTTTATATCCATCCTTCCGACGACCACTATGCTAAAATCCACA
AAAAATAAAGTGGATAGAAC

Downstream 100 bases:

>100_bases
TACTCTTTGCAGATGAACGAATACCAGATTACCTTTTTTGTCGATGATATTAACGCCAGTGCTCATGTTGCACAGCCTCT
CAATCACGTCGCCAAAAAGT

Product: hypothetical protein

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MKIVAVTACPTGIAHTYMAADALMKAAPKHNVQIKVETQGAMGIENQLTPHDIAQADRVLIVSDIEIEQPARFEGTNKVQ
IPIEDVLLNVDKVFLVHYR

Sequences:

>Translated_99_residues
MKIVAVTACPTGIAHTYMAADALMKAAPKHNVQIKVETQGAMGIENQLTPHDIAQADRVLIVSDIEIEQPARFEGTNKVQ
IPIEDVLLNVDKVFLVHYR
>Mature_99_residues
MKIVAVTACPTGIAHTYMAADALMKAAPKHNVQIKVETQGAMGIENQLTPHDIAQADRVLIVSDIEIEQPARFEGTNKVQ
IPIEDVLLNVDKVFLVHYR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=93, Percent_Identity=49.4623655913978, Blast_Score=100, Evalue=1e-23,
Organism=Escherichia coli, GI87082348, Length=96, Percent_Identity=44.7916666666667, Blast_Score=85, Evalue=9e-19,
Organism=Escherichia coli, GI1790390, Length=92, Percent_Identity=44.5652173913043, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI1788492, Length=74, Percent_Identity=50, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1788730, Length=88, Percent_Identity=43.1818181818182, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1786951, Length=83, Percent_Identity=44.578313253012, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 10905; Mature: 10905

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVAVTACPTGIAHTYMAADALMKAAPKHNVQIKVETQGAMGIENQLTPHDIAQADRVL
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEE
IVSDIEIEQPARFEGTNKVQIPIEDVLLNVDKVFLVHYR
EEECCCCCCCCCCCCCCEEEEEHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MKIVAVTACPTGIAHTYMAADALMKAAPKHNVQIKVETQGAMGIENQLTPHDIAQADRVL
CEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEE
IVSDIEIEQPARFEGTNKVQIPIEDVLLNVDKVFLVHYR
EEECCCCCCCCCCCCCCEEEEEHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]