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The map label for this gene is gcvA [H]

Identifier: 156976209

GI number: 156976209

Start: 237239

End: 238174

Strand: Reverse

Name: gcvA [H]

Synonym: VIBHAR_04980

Alternate gene names: 156976209

Gene position: 238174-237239 (Counterclockwise)

Preceding gene: 156976216

Following gene: 156976208

Centisome position: 10.81

GC content: 45.83

Gene sequence:

>936_bases
ATGAAAAAAGAAGGCATTAGGAGTAAAGATACCATGCGCAAGTTAGTCCCTTTGAAGTCCATCTACGCCTTTGTCGCGGT
TGCCGAGACAGGCAGCATGACAGAAGCCGCTCAACTACTCTCTGTAAGCCATTCCGCTGTGAGTCAAGCCATCAAATCTC
TCGAAAGCCAAGTGAATAAACCTCTGTTCGATCGAGTCGGTCGCCAAGTTCGTCTCAATAACGACGGCAAAAAGTATTAC
CGCAAAGTCGCCCCGGCACTTGAGCAGATCGTTGATGCAACAGAAGCGCTCCTTCACGACCAAAATTCAAAACGGATTAC
GTTTAATATGGCCAACTCTCTGGCCCTGCATTGGTGGATTCCTAGAATGTCGAGTTTACAAGACTTCGCTCCGCAACTTG
ATATCCGCTTATCCAACCTTATTGGGCAGTTCAGTTTGGAGCAAGAAGGTATTGATGCTGCATTGGTGCACGGTAGCCCT
CAAGAGTGGCAAGATTACTACTGCGAGAAGTTGAGCGACGATGAGTTAGTCCTGGTGTGCAGCCCAGAACTATTAGATCA
ACCAAATAGCCAAGACATCAAACAGCTGTTATCAACCTACCCAATTATTGAAGTCACCAATGACCGCCGCAAGCACGATT
GGCAAATCTGGCAAGAACAAACTGGCATAGAAAGGCCAAAGGGAAAAAAACCGGTTACCTTTGACATGTCCATTCAAGCT
GTACAAGCAACTATTCGCCGTCTGGGCTTACTGGTCACACATCGATTGTTCGTAACAGATGACATCAAATACGGCCAGTT
GGTAGAACTAGGGCGGCCTATCGTTAATCCATCCCAACAATTCTACTTGATATGTCCACCACATAAACTTAAGCATGAAG
CCTTTCATTTGTTACGTTCTTGGCTACGACAAGAATTTAGTCAAACGAAAGTGTAA

Upstream 100 bases:

>100_bases
CTTAATTCACGGAGTATTTTAAGAAAAGGCACAAGCGAGAAATAGCGAGTATTTTTAAACGCTAATGTAAGTAAAACTTA
CAAAATAAAGTTATTTAAAG

Downstream 100 bases:

>100_bases
CTTGTTGAGTATTAGCTAGGCATTAAGACATTTATTCGTTTTCTAGGTGAATAGAATAATGCCGGAATTGGTATAAAGTA
AGAAGAGTACTTTCAAACCC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKKEGIRSKDTMRKLVPLKSIYAFVAVAETGSMTEAAQLLSVSHSAVSQAIKSLESQVNKPLFDRVGRQVRLNNDGKKYY
RKVAPALEQIVDATEALLHDQNSKRITFNMANSLALHWWIPRMSSLQDFAPQLDIRLSNLIGQFSLEQEGIDAALVHGSP
QEWQDYYCEKLSDDELVLVCSPELLDQPNSQDIKQLLSTYPIIEVTNDRRKHDWQIWQEQTGIERPKGKKPVTFDMSIQA
VQATIRRLGLLVTHRLFVTDDIKYGQLVELGRPIVNPSQQFYLICPPHKLKHEAFHLLRSWLRQEFSQTKV

Sequences:

>Translated_311_residues
MKKEGIRSKDTMRKLVPLKSIYAFVAVAETGSMTEAAQLLSVSHSAVSQAIKSLESQVNKPLFDRVGRQVRLNNDGKKYY
RKVAPALEQIVDATEALLHDQNSKRITFNMANSLALHWWIPRMSSLQDFAPQLDIRLSNLIGQFSLEQEGIDAALVHGSP
QEWQDYYCEKLSDDELVLVCSPELLDQPNSQDIKQLLSTYPIIEVTNDRRKHDWQIWQEQTGIERPKGKKPVTFDMSIQA
VQATIRRLGLLVTHRLFVTDDIKYGQLVELGRPIVNPSQQFYLICPPHKLKHEAFHLLRSWLRQEFSQTKV
>Mature_311_residues
MKKEGIRSKDTMRKLVPLKSIYAFVAVAETGSMTEAAQLLSVSHSAVSQAIKSLESQVNKPLFDRVGRQVRLNNDGKKYY
RKVAPALEQIVDATEALLHDQNSKRITFNMANSLALHWWIPRMSSLQDFAPQLDIRLSNLIGQFSLEQEGIDAALVHGSP
QEWQDYYCEKLSDDELVLVCSPELLDQPNSQDIKQLLSTYPIIEVTNDRRKHDWQIWQEQTGIERPKGKKPVTFDMSIQA
VQATIRRLGLLVTHRLFVTDDIKYGQLVELGRPIVNPSQQFYLICPPHKLKHEAFHLLRSWLRQEFSQTKV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=27.9310344827586, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786448, Length=301, Percent_Identity=27.906976744186, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=25.752508361204, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786401, Length=267, Percent_Identity=24.3445692883895, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788481, Length=264, Percent_Identity=26.5151515151515, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693193, Length=250, Percent_Identity=22.4, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=82, Percent_Identity=42.6829268292683, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35688; Mature: 35688

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEGIRSKDTMRKLVPLKSIYAFVAVAETGSMTEAAQLLSVSHSAVSQAIKSLESQVNK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLFDRVGRQVRLNNDGKKYYRKVAPALEQIVDATEALLHDQNSKRITFNMANSLALHWWI
CHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
PRMSSLQDFAPQLDIRLSNLIGQFSLEQEGIDAALVHGSPQEWQDYYCEKLSDDELVLVC
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEE
SPELLDQPNSQDIKQLLSTYPIIEVTNDRRKHDWQIWQEQTGIERPKGKKPVTFDMSIQA
CHHHHCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECHHHHH
VQATIRRLGLLVTHRLFVTDDIKYGQLVELGRPIVNPSQQFYLICPPHKLKHEAFHLLRS
HHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
WLRQEFSQTKV
HHHHHHHHCCC
>Mature Secondary Structure
MKKEGIRSKDTMRKLVPLKSIYAFVAVAETGSMTEAAQLLSVSHSAVSQAIKSLESQVNK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLFDRVGRQVRLNNDGKKYYRKVAPALEQIVDATEALLHDQNSKRITFNMANSLALHWWI
CHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
PRMSSLQDFAPQLDIRLSNLIGQFSLEQEGIDAALVHGSPQEWQDYYCEKLSDDELVLVC
CCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEE
SPELLDQPNSQDIKQLLSTYPIIEVTNDRRKHDWQIWQEQTGIERPKGKKPVTFDMSIQA
CHHHHCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECHHHHH
VQATIRRLGLLVTHRLFVTDDIKYGQLVELGRPIVNPSQQFYLICPPHKLKHEAFHLLRS
HHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
WLRQEFSQTKV
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]