Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 156938015

GI number: 156938015

Start: 1094163

End: 1095656

Strand: Reverse

Name: glnA [H]

Synonym: Igni_1228

Alternate gene names: 156938015

Gene position: 1095656-1094163 (Counterclockwise)

Preceding gene: 156938018

Following gene: 156938014

Centisome position: 84.44

GC content: 57.83

Gene sequence:

>1494_bases
GTGTCACGCGAGGAGTTGGTTAAGGAAGTAGTGGAGAGCATGAAAACCGAGAACGTCAGGTGGGTGATGGTCCAGTTCTT
GGACGTCCCCGGAAGGTTGCAACAAATGACCTTCCCCGCGCGGGAGATACTGGACGACCCGGAGAACGCCTTCAACGTCG
CCGTTGGGGGCTTTGACGGCTCCTCCATAAAGGGCTTCACTATGATTAACGAGAGCGACATGCTCTTGAGGCCCGTGCCG
GAGACCTTCGCAATAATTCCGGACGACTGGCAGAGCCCTCCGGGGGTGGCCGCGGGGAGGACCGCTAGGTTCTTGGCTCA
GATCTTCTGGGGAGGTTATAAGAAGGGCGAAGCGAAGAGGTTCGAGAAGGACCCGAGGTACATAGCGGAGAGGGCCGAGG
AGTACCTAGCTGAGCAAGGGTACAAGGCCTACTTCGGTCCCGAAGTGGAGTTCATGATACTCAACAAAGTAATCAATGAG
GTGGACAGACCTTGGAAGAAGATGCACGTGGAGGTGGTCTCTGCCGAGGCCGCGTGGAACACGGAGCTCCCGATCATAAG
GTTCGAGGAAGGTTACGTGCCGGCCATACCATACGACAAGATGTTCTGGATAAGGCAAGAGATAGCTACCGTGCTCGAGG
ACCATTTCGGGATACAAGTGGAGGCTCACCACCACGAGGTGGCCTCCCCCGGCCAAGGCGAGATAGACTTCCGTTTCGAC
ACGCTCACCAGAGCGGCTGACAAGGTAGTCACCTTGAAGTTCGTGGTAAAGAACGTGGCTGCCAAGTACGGCCTGGTAGC
GACGTTCATGCCCAAGCCCGTCTACGCCGACAACGGAAACGGCATGCACACCCACCAGAGCTTGTGGAGCAAGGACAAAG
ACGAGAACTTGTTCTACGACCCCAACGACGAGTACGCGGAGCTGAGCCAGCTGGCTAGGTACTACATAGGAGGTATCTTG
AAGCACGCCAGGGCGCTCAGCGCGATAGTCAACCCGACCACCTCCAGCTACAAGAGGCTGGTGCCGGGCTACGAGGCCCC
CATATACATAGCTTGGAGCAAGGCCAACCGCTCCGCCATAATCAGGGTGCCGAACTACATGAGGGGAGTGCCGAAGGCGG
CGAGGATAGAGTACCGCTCCCCCGACCCCAGCACCAACCCCTACTTGGCCTTCGCGGCGATGGTTGCCGCGGGCTTGGAC
GGCATCAAGAAGAAGATCGAGCCCCCGCCGCCGGTGGACCAGAACATATACGCCATGGACCCGAAGACGAGGAGGGAGTT
GGAAGAGGTAGTGAGGAAGCAGCTCGGCACCCCCTTGCAGCTGCCGAGGACCTTGTGCGAGGCGATAGAGGAGCTGGAGA
ACGACCACGACTGGCTGTTCCCCATATTCACCAAGGAGATGTTGGAGACTTGGATAGAGATGAAGAAAGAGGAGTGCTTG
AAGATAGAGAGCTACCCGCACCCGGCGGAGTTCTACCAGTACTTCGACATATGA

Upstream 100 bases:

>100_bases
AAACGTTTATAGAAATTGACAATTTTGTGAACATTGTTCAGTAATATTGGGATAGATGATAAATACTTGTTTATCAGTGA
AGGGGAACGCGGGAACAGTT

Downstream 100 bases:

>100_bases
GGCGTCGGAAGAGGGCCTCTCCTCACCGCTCGAGCGGCCTAGCGCTCCTCATCCGCCTTTTTAGTCCCCCAAGTTATGTC
GTAGGGACCTTTCTTGATAA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSREELVKEVVESMKTENVRWVMVQFLDVPGRLQQMTFPAREILDDPENAFNVAVGGFDGSSIKGFTMINESDMLLRPVP
ETFAIIPDDWQSPPGVAAGRTARFLAQIFWGGYKKGEAKRFEKDPRYIAERAEEYLAEQGYKAYFGPEVEFMILNKVINE
VDRPWKKMHVEVVSAEAAWNTELPIIRFEEGYVPAIPYDKMFWIRQEIATVLEDHFGIQVEAHHHEVASPGQGEIDFRFD
TLTRAADKVVTLKFVVKNVAAKYGLVATFMPKPVYADNGNGMHTHQSLWSKDKDENLFYDPNDEYAELSQLARYYIGGIL
KHARALSAIVNPTTSSYKRLVPGYEAPIYIAWSKANRSAIIRVPNYMRGVPKAARIEYRSPDPSTNPYLAFAAMVAAGLD
GIKKKIEPPPPVDQNIYAMDPKTRRELEEVVRKQLGTPLQLPRTLCEAIEELENDHDWLFPIFTKEMLETWIEMKKEECL
KIESYPHPAEFYQYFDI

Sequences:

>Translated_497_residues
MSREELVKEVVESMKTENVRWVMVQFLDVPGRLQQMTFPAREILDDPENAFNVAVGGFDGSSIKGFTMINESDMLLRPVP
ETFAIIPDDWQSPPGVAAGRTARFLAQIFWGGYKKGEAKRFEKDPRYIAERAEEYLAEQGYKAYFGPEVEFMILNKVINE
VDRPWKKMHVEVVSAEAAWNTELPIIRFEEGYVPAIPYDKMFWIRQEIATVLEDHFGIQVEAHHHEVASPGQGEIDFRFD
TLTRAADKVVTLKFVVKNVAAKYGLVATFMPKPVYADNGNGMHTHQSLWSKDKDENLFYDPNDEYAELSQLARYYIGGIL
KHARALSAIVNPTTSSYKRLVPGYEAPIYIAWSKANRSAIIRVPNYMRGVPKAARIEYRSPDPSTNPYLAFAAMVAAGLD
GIKKKIEPPPPVDQNIYAMDPKTRRELEEVVRKQLGTPLQLPRTLCEAIEELENDHDWLFPIFTKEMLETWIEMKKEECL
KIESYPHPAEFYQYFDI
>Mature_496_residues
SREELVKEVVESMKTENVRWVMVQFLDVPGRLQQMTFPAREILDDPENAFNVAVGGFDGSSIKGFTMINESDMLLRPVPE
TFAIIPDDWQSPPGVAAGRTARFLAQIFWGGYKKGEAKRFEKDPRYIAERAEEYLAEQGYKAYFGPEVEFMILNKVINEV
DRPWKKMHVEVVSAEAAWNTELPIIRFEEGYVPAIPYDKMFWIRQEIATVLEDHFGIQVEAHHHEVASPGQGEIDFRFDT
LTRAADKVVTLKFVVKNVAAKYGLVATFMPKPVYADNGNGMHTHQSLWSKDKDENLFYDPNDEYAELSQLARYYIGGILK
HARALSAIVNPTTSSYKRLVPGYEAPIYIAWSKANRSAIIRVPNYMRGVPKAARIEYRSPDPSTNPYLAFAAMVAAGLDG
IKKKIEPPPPVDQNIYAMDPKTRRELEEVVRKQLGTPLQLPRTLCEAIEELENDHDWLFPIFTKEMLETWIEMKKEECLK
IESYPHPAEFYQYFDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=503, Percent_Identity=25.6461232604374, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1790301, Length=505, Percent_Identity=41.5841584158416, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87081870, Length=258, Percent_Identity=30.6201550387597, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 56993; Mature: 56861

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREELVKEVVESMKTENVRWVMVQFLDVPGRLQQMTFPAREILDDPENAFNVAVGGFDG
CCHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHCCCHHHHHCCCCCCEEEEEECCCC
SSIKGFTMINESDMLLRPVPETFAIIPDDWQSPPGVAAGRTARFLAQIFWGGYKKGEAKR
CCCCEEEEECCCCCEEECCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
FEKDPRYIAERAEEYLAEQGYKAYFGPEVEFMILNKVINEVDRPWKKMHVEVVSAEAAWN
CCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCC
TELPIIRFEEGYVPAIPYDKMFWIRQEIATVLEDHFGIQVEAHHHEVASPGQGEIDFRFD
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEHH
TLTRAADKVVTLKFVVKNVAAKYGLVATFMPKPVYADNGNGMHTHQSLWSKDKDENLFYD
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEECCCCCCCCHHHHHHCCCCCCCCCCC
PNDEYAELSQLARYYIGGILKHARALSAIVNPTTSSYKRLVPGYEAPIYIAWSKANRSAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCEE
IRVPNYMRGVPKAARIEYRSPDPSTNPYLAFAAMVAAGLDGIKKKIEPPPPVDQNIYAMD
EECCHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
PKTRRELEEVVRKQLGTPLQLPRTLCEAIEELENDHDWLFPIFTKEMLETWIEMKKEECL
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
KIESYPHPAEFYQYFDI
CCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SREELVKEVVESMKTENVRWVMVQFLDVPGRLQQMTFPAREILDDPENAFNVAVGGFDG
CHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHCCCHHHHHCCCCCCEEEEEECCCC
SSIKGFTMINESDMLLRPVPETFAIIPDDWQSPPGVAAGRTARFLAQIFWGGYKKGEAKR
CCCCEEEEECCCCCEEECCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
FEKDPRYIAERAEEYLAEQGYKAYFGPEVEFMILNKVINEVDRPWKKMHVEVVSAEAAWN
CCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEECCCCC
TELPIIRFEEGYVPAIPYDKMFWIRQEIATVLEDHFGIQVEAHHHEVASPGQGEIDFRFD
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEHH
TLTRAADKVVTLKFVVKNVAAKYGLVATFMPKPVYADNGNGMHTHQSLWSKDKDENLFYD
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEECCCCCCCCHHHHHHCCCCCCCCCCC
PNDEYAELSQLARYYIGGILKHARALSAIVNPTTSSYKRLVPGYEAPIYIAWSKANRSAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCEE
IRVPNYMRGVPKAARIEYRSPDPSTNPYLAFAAMVAAGLDGIKKKIEPPPPVDQNIYAMD
EECCHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
PKTRRELEEVVRKQLGTPLQLPRTLCEAIEELENDHDWLFPIFTKEMLETWIEMKKEECL
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
KIESYPHPAEFYQYFDI
CCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973523; 11427726 [H]