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The map label for this gene is pheS [H]

Identifier: 156938014

GI number: 156938014

Start: 1092633

End: 1094069

Strand: Reverse

Name: pheS [H]

Synonym: Igni_1227

Alternate gene names: 156938014

Gene position: 1094069-1092633 (Counterclockwise)

Preceding gene: 156938015

Following gene: 156938013

Centisome position: 84.32

GC content: 58.32

Gene sequence:

>1437_bases
TTGATAAGGGTGCCCGAGGAACAGTACCGCTTGCTCGTGTCACTCAGCAAGGACGGAGAGGACCTCGAAACGGTGGCCAA
GAAGTTAGGAAAGAGGAAGGAAGAACTCATGAGGCCCCTGGCGGAACTGGAGAAGAAGGGGTTAGTGGAGGTTGAGCGCG
TCGAGGTGGAAACTTTCGAGCTTAACGAGAAGGGGAAGAAGTATCTGAGCGAAAGGTTGCCGGAGGAGAAGTTGTTAGAG
ATGGGGGAGTGTGTCGAGCTGAGCGAGCTCAAGTCGAAGCTCGGGCCCGAGGCGGGGGTCGCGATAGGCCTCGCTAAGCG
CTTGGGGGGCGAAATAAGGGAAGGCAAGCTGTGTTACGACGCGAATGTGTTGAAGGAGTACGCCTCGAGGGTTAGGAGGT
GCTTGGAGGAGGGGAGCCCCTGCGAGGAGCTCCGGAAGAGGGGGTTCTTAAGGAAGGTCAAGGTGAAGAAGATAATAATA
AAACCTTCGGAGTTGGCCTTGAAGGCCTTGGAGCGCGGGGAGCTAGTCCCGAGTAAGACGGTTACCGCCCTAACTAGGAA
GGACTTAATAGAGGGGACTTGGAAAGACGCCGAGTTCAAGAAGTTTGACTTGAGCGTGGAGTTCCCCGCGAGGCTTCCCA
AGAGGAGGCACCCGCTATTGGAGTTCCACGAGTACGTGAAGGAGGTCTTGGTATCTATGGGGTTCGAGGAGGTCGTGGGG
GACTACGTAACCAACGCCTTCTGGAACTTCGACGTGCTCTTGGTCCCCCAGTTCCACCCGGCCCGCTCGGAGACAGACGT
GTTCTACGCGTCCGGGGCCCGGGTGCCTCCCCCGCCCGAGGGGCTCTTGGAGGAGGTCGCGGAGGTGCACCGGGAGATGT
GGGGCTACTGGAGGGCCGAAGTTGCGTTGAAGGTCCTCTTGAGGACCCACACCACCGTGGTAAGCGCGCAGACCATGTAC
TCCCGTGGGGGAGGGGAGTACAGAGCGTTCTCCTTGGACAGGGTGTTTAGGTCAGAGACATTGGACCCGACGCACTCCAT
GGAGTTCCACCAGCTCGAGGGGATAATAGTGGGGAAGGAGGTGTCGTTCAAGCACTTGTTGGGCTTCTTCAAGGAGTTCG
CTAAGAAGCTCGGCTTGGGGGAGGTCAAGTTCAAGCCGGCTTACTTCCCCTTTACCGAGCCCTCCGTGGAGGGCTTCGTC
AAGCACCCTGAGCTGGGCTGGGTCGAGGTCTTCCCGGGAGGGATGTTCAGGCCGGAGATGTTGAAGCCGTTGGGCCTCGG
GGACTTCTCCGTGGCGGCTTGGGGGATAGGGATAGACAGGCTCGCCATGCTGGCCTTGGGGCTCTCTGACATAAGGGACT
TGTACTCCAACGACTTGATAAAGCTGAGGAAGGTGAAGCCTATAAAGGGCCTGTGGTCGGGGAAGGCGTTATTTTGA

Upstream 100 bases:

>100_bases
CATATGAGGCGTCGGAAGAGGGCCTCTCCTCACCGCTCGAGCGGCCTAGCGCTCCTCATCCGCCTTTTTAGTCCCCCAAG
TTATGTCGTAGGGACCTTTC

Downstream 100 bases:

>100_bases
TTCCTTTACATCCCCCAGGGGAGAGGCTTGCCAGTAGTCACGGTCAAGCTGTGGGACTTGGAGCGCCTCGCCCGAGGGCC
TCTGTCCGAGGACCTCCGGC

Product: phenylalanyl-tRNA synthetase, alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MIRVPEEQYRLLVSLSKDGEDLETVAKKLGKRKEELMRPLAELEKKGLVEVERVEVETFELNEKGKKYLSERLPEEKLLE
MGECVELSELKSKLGPEAGVAIGLAKRLGGEIREGKLCYDANVLKEYASRVRRCLEEGSPCEELRKRGFLRKVKVKKIII
KPSELALKALERGELVPSKTVTALTRKDLIEGTWKDAEFKKFDLSVEFPARLPKRRHPLLEFHEYVKEVLVSMGFEEVVG
DYVTNAFWNFDVLLVPQFHPARSETDVFYASGARVPPPPEGLLEEVAEVHREMWGYWRAEVALKVLLRTHTTVVSAQTMY
SRGGGEYRAFSLDRVFRSETLDPTHSMEFHQLEGIIVGKEVSFKHLLGFFKEFAKKLGLGEVKFKPAYFPFTEPSVEGFV
KHPELGWVEVFPGGMFRPEMLKPLGLGDFSVAAWGIGIDRLAMLALGLSDIRDLYSNDLIKLRKVKPIKGLWSGKALF

Sequences:

>Translated_478_residues
MIRVPEEQYRLLVSLSKDGEDLETVAKKLGKRKEELMRPLAELEKKGLVEVERVEVETFELNEKGKKYLSERLPEEKLLE
MGECVELSELKSKLGPEAGVAIGLAKRLGGEIREGKLCYDANVLKEYASRVRRCLEEGSPCEELRKRGFLRKVKVKKIII
KPSELALKALERGELVPSKTVTALTRKDLIEGTWKDAEFKKFDLSVEFPARLPKRRHPLLEFHEYVKEVLVSMGFEEVVG
DYVTNAFWNFDVLLVPQFHPARSETDVFYASGARVPPPPEGLLEEVAEVHREMWGYWRAEVALKVLLRTHTTVVSAQTMY
SRGGGEYRAFSLDRVFRSETLDPTHSMEFHQLEGIIVGKEVSFKHLLGFFKEFAKKLGLGEVKFKPAYFPFTEPSVEGFV
KHPELGWVEVFPGGMFRPEMLKPLGLGDFSVAAWGIGIDRLAMLALGLSDIRDLYSNDLIKLRKVKPIKGLWSGKALF
>Mature_478_residues
MIRVPEEQYRLLVSLSKDGEDLETVAKKLGKRKEELMRPLAELEKKGLVEVERVEVETFELNEKGKKYLSERLPEEKLLE
MGECVELSELKSKLGPEAGVAIGLAKRLGGEIREGKLCYDANVLKEYASRVRRCLEEGSPCEELRKRGFLRKVKVKKIII
KPSELALKALERGELVPSKTVTALTRKDLIEGTWKDAEFKKFDLSVEFPARLPKRRHPLLEFHEYVKEVLVSMGFEEVVG
DYVTNAFWNFDVLLVPQFHPARSETDVFYASGARVPPPPEGLLEEVAEVHREMWGYWRAEVALKVLLRTHTTVVSAQTMY
SRGGGEYRAFSLDRVFRSETLDPTHSMEFHQLEGIIVGKEVSFKHLLGFFKEFAKKLGLGEVKFKPAYFPFTEPSVEGFV
KHPELGWVEVFPGGMFRPEMLKPLGLGDFSVAAWGIGIDRLAMLALGLSDIRDLYSNDLIKLRKVKPIKGLWSGKALF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=471, Percent_Identity=32.9087048832272, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1788007, Length=287, Percent_Identity=30.3135888501742, Blast_Score=132, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32563657, Length=440, Percent_Identity=35.4545454545455, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17508957, Length=440, Percent_Identity=35.4545454545455, Blast_Score=244, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321087, Length=478, Percent_Identity=31.1715481171548, Blast_Score=217, Evalue=4e-57,
Organism=Drosophila melanogaster, GI18858079, Length=489, Percent_Identity=32.1063394683027, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 54387; Mature: 54387

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVPEEQYRLLVSLSKDGEDLETVAKKLGKRKEELMRPLAELEKKGLVEVERVEVETFE
CCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
LNEKGKKYLSERLPEEKLLEMGECVELSELKSKLGPEAGVAIGLAKRLGGEIREGKLCYD
CCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEC
ANVLKEYASRVRRCLEEGSPCEELRKRGFLRKVKVKKIIIKPSELALKALERGELVPSKT
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHEEECCHHHHHHHHHHCCCCCCHH
VTALTRKDLIEGTWKDAEFKKFDLSVEFPARLPKRRHPLLEFHEYVKEVLVSMGFEEVVG
HHHHHHHHHHCCCCCCCCCEEEEEEEECHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
DYVTNAFWNFDVLLVPQFHPARSETDVFYASGARVPPPPEGLLEEVAEVHREMWGYWRAE
HHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALKVLLRTHTTVVSAQTMYSRGGGEYRAFSLDRVFRSETLDPTHSMEFHQLEGIIVGKE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHCCEEEECC
VSFKHLLGFFKEFAKKLGLGEVKFKPAYFPFTEPSVEGFVKHPELGWVEVFPGGMFRPEM
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCHHH
LKPLGLGDFSVAAWGIGIDRLAMLALGLSDIRDLYSNDLIKLRKVKPIKGLWSGKALF
HCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRVPEEQYRLLVSLSKDGEDLETVAKKLGKRKEELMRPLAELEKKGLVEVERVEVETFE
CCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
LNEKGKKYLSERLPEEKLLEMGECVELSELKSKLGPEAGVAIGLAKRLGGEIREGKLCYD
CCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEC
ANVLKEYASRVRRCLEEGSPCEELRKRGFLRKVKVKKIIIKPSELALKALERGELVPSKT
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHEEECCHHHHHHHHHHCCCCCCHH
VTALTRKDLIEGTWKDAEFKKFDLSVEFPARLPKRRHPLLEFHEYVKEVLVSMGFEEVVG
HHHHHHHHHHCCCCCCCCCEEEEEEEECHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
DYVTNAFWNFDVLLVPQFHPARSETDVFYASGARVPPPPEGLLEEVAEVHREMWGYWRAE
HHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALKVLLRTHTTVVSAQTMYSRGGGEYRAFSLDRVFRSETLDPTHSMEFHQLEGIIVGKE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHCCEEEECC
VSFKHLLGFFKEFAKKLGLGEVKFKPAYFPFTEPSVEGFVKHPELGWVEVFPGGMFRPEM
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCHHH
LKPLGLGDFSVAAWGIGIDRLAMLALGLSDIRDLYSNDLIKLRKVKPIKGLWSGKALF
HCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA