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The map label for this gene is leuC [H]

Identifier: 156937875

GI number: 156937875

Start: 969129

End: 970418

Strand: Reverse

Name: leuC [H]

Synonym: Igni_1087

Alternate gene names: 156937875

Gene position: 970418-969129 (Counterclockwise)

Preceding gene: 156937876

Following gene: 156937874

Centisome position: 74.79

GC content: 60.54

Gene sequence:

>1290_bases
TTGCCGAGCACGATTGCAGAGAAGATACTGGGGGCCAAGGTCGGTAAGGAAGTGAGCCCGGGCGAGCTGGTCGTAGTGGA
CGTGGACGGAGTAATGGCCCAAGACGGGACCGCCCCGCTTGCCATCAAAGTAATGAGGGAGAGGTTCGGGGAGGAGAGGG
TCTTCGACCCCGAGAAGGTAGCCTTCGTGATAGACCACACCGCCCCCAGCAACAACCCCGGCACCTCAGAGCTTCACATA
GAGATGAGGAGGTTCTGTAGGAAGCACAAGTGCACCCTCTACGACGTGGGCACCGGGATATGCCACCAAGTGATGGTGGA
GGAGGGGAGGGCGTGGCCCGGCTCGGTGTTCGTGGGGGCGGACTCCCACACAGTAACCTACGGCGCCCTGGGCTCCTTTG
CCACCGGCGTGGGCTCCACAGACGCCGCCATAGCGATGATGACCGGTAGGATATGGTTCAAGGTCCCGGAGGCGCTCAAG
TTCAACCTGATCGGCAAGTTTAGGGAGGGTGTAATGAGTAAAGACCTAATACTGAAGATAATAGGCGACTTGAAGGAGGA
CGGAGCGACCTACATGAGCGCGGAGTTCGTCGGCGACGGCTTGAAGGACATGAGGATAGACTCCAGGCTGACGGTGAGCA
ACATGGTGGTCGAGATGGGAGCCAAGGTAGGCCTCATGCCCGCCGACGAAGAAGTCATGAGGTTCGTGGAGGGCCGGGCT
AGGGGAGTCCCTAAGCCCGAGCTCACCTTCCCCGACCCCGGGGCCCACTACAAGGACGAGTTCACCTACGACTTGAGCGA
GATAGAGCCGATGGTGGCAAAGCCCTACTCGCCGGCGAACTCCGTTCCGGTGAGCGAGGTGGAGGGCATAGAGGTGGACC
AAGTGTTCATAGGCAGCTGTACCAACGGCAGGCTCGAAGACGTGGCAGTGGCCGCGAGGATACTGAAGGGGAAGAAGGTG
GCCCCGAACACTAGGTGTATAGTCATAACTGCCTCCAGGAACGTGTACTTGCAAGCGCTCAAGAAGGGCTACGTGGACGT
GCTCACCGAGGCCGGGTGCTTGGTCACCTTCGGCACTTGCGGCCCGTGCGTTGGGGCACACTACGGAGTACTGGGCCCCA
ACGAGGTGGGCTTGTTCACTACCAATAGAAACTTCAGGGGGAGGAGCGGGCACCGGACGAGCAAGGTCTACCTAGCGAGC
CCCGCCACTGCGGCGGCGAGCGCGGTGGAGGGCAAGATAGCTGACCCGCGGAAGTACTTGAAGGTCAGAGTTAGGGACAT
GGAGGTCTGA

Upstream 100 bases:

>100_bases
TGGTCGCGACCCTCAAGGCCCTCCCCGCGGGGACCTTGCCCCTGAGCCCGTGAGGGGGAAAACACTTATCCCTCTCCCAA
AACCCCTTTGGGGAAATACC

Downstream 100 bases:

>100_bases
GCTCGGGGCCGGTCCACCTCCTCACCGCCTCGGACTAGGTCCCGTAACCCTCATCGCTGAAAACCTCTCGCTCGCCCGGG
GGGCTACGGGTCAAAAGGTT

Product: 3-isopropylmalate dehydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPSTIAEKILGAKVGKEVSPGELVVVDVDGVMAQDGTAPLAIKVMRERFGEERVFDPEKVAFVIDHTAPSNNPGTSELHI
EMRRFCRKHKCTLYDVGTGICHQVMVEEGRAWPGSVFVGADSHTVTYGALGSFATGVGSTDAAIAMMTGRIWFKVPEALK
FNLIGKFREGVMSKDLILKIIGDLKEDGATYMSAEFVGDGLKDMRIDSRLTVSNMVVEMGAKVGLMPADEEVMRFVEGRA
RGVPKPELTFPDPGAHYKDEFTYDLSEIEPMVAKPYSPANSVPVSEVEGIEVDQVFIGSCTNGRLEDVAVAARILKGKKV
APNTRCIVITASRNVYLQALKKGYVDVLTEAGCLVTFGTCGPCVGAHYGVLGPNEVGLFTTNRNFRGRSGHRTSKVYLAS
PATAAASAVEGKIADPRKYLKVRVRDMEV

Sequences:

>Translated_429_residues
MPSTIAEKILGAKVGKEVSPGELVVVDVDGVMAQDGTAPLAIKVMRERFGEERVFDPEKVAFVIDHTAPSNNPGTSELHI
EMRRFCRKHKCTLYDVGTGICHQVMVEEGRAWPGSVFVGADSHTVTYGALGSFATGVGSTDAAIAMMTGRIWFKVPEALK
FNLIGKFREGVMSKDLILKIIGDLKEDGATYMSAEFVGDGLKDMRIDSRLTVSNMVVEMGAKVGLMPADEEVMRFVEGRA
RGVPKPELTFPDPGAHYKDEFTYDLSEIEPMVAKPYSPANSVPVSEVEGIEVDQVFIGSCTNGRLEDVAVAARILKGKKV
APNTRCIVITASRNVYLQALKKGYVDVLTEAGCLVTFGTCGPCVGAHYGVLGPNEVGLFTTNRNFRGRSGHRTSKVYLAS
PATAAASAVEGKIADPRKYLKVRVRDMEV
>Mature_428_residues
PSTIAEKILGAKVGKEVSPGELVVVDVDGVMAQDGTAPLAIKVMRERFGEERVFDPEKVAFVIDHTAPSNNPGTSELHIE
MRRFCRKHKCTLYDVGTGICHQVMVEEGRAWPGSVFVGADSHTVTYGALGSFATGVGSTDAAIAMMTGRIWFKVPEALKF
NLIGKFREGVMSKDLILKIIGDLKEDGATYMSAEFVGDGLKDMRIDSRLTVSNMVVEMGAKVGLMPADEEVMRFVEGRAR
GVPKPELTFPDPGAHYKDEFTYDLSEIEPMVAKPYSPANSVPVSEVEGIEVDQVFIGSCTNGRLEDVAVAARILKGKKVA
PNTRCIVITASRNVYLQALKKGYVDVLTEAGCLVTFGTCGPCVGAHYGVLGPNEVGLFTTNRNFRGRSGHRTSKVYLASP
ATAAASAVEGKIADPRKYLKVRVRDMEV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=450, Percent_Identity=28.4444444444444, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI8659555, Length=403, Percent_Identity=26.302729528536, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=25.0632911392405, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=31.7021276595745, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI87081781, Length=352, Percent_Identity=29.8295454545455, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787531, Length=501, Percent_Identity=25.9481037924152, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI2367097, Length=460, Percent_Identity=25.2173913043478, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=448, Percent_Identity=26.7857142857143, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=27.9005524861878, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=398, Percent_Identity=25.6281407035176, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25149342, Length=293, Percent_Identity=26.2798634812287, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320440, Length=432, Percent_Identity=34.7222222222222, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=28.2105263157895, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=450, Percent_Identity=29.5555555555556, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6322261, Length=459, Percent_Identity=27.8867102396514, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28571643, Length=448, Percent_Identity=28.125, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365315, Length=457, Percent_Identity=27.5711159737418, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=457, Percent_Identity=27.5711159737418, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=420, Percent_Identity=28.0952380952381, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17137564, Length=403, Percent_Identity=27.0471464019851, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645686, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 46388; Mature: 46257

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTIAEKILGAKVGKEVSPGELVVVDVDGVMAQDGTAPLAIKVMRERFGEERVFDPEKV
CCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCE
AFVIDHTAPSNNPGTSELHIEMRRFCRKHKCTLYDVGTGICHQVMVEEGRAWPGSVFVGA
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEC
DSHTVTYGALGSFATGVGSTDAAIAMMTGRIWFKVPEALKFNLIGKFREGVMSKDLILKI
CCCEEEEECHHHHHCCCCCCCCEEEEEECEEEEECCCHHEEHHHHHHHHCCCCHHHHHHH
IGDLKEDGATYMSAEFVGDGLKDMRIDSRLTVSNMVVEMGAKVGLMPADEEVMRFVEGRA
HHHHHHCCCCEEEHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RGVPKPELTFPDPGAHYKDEFTYDLSEIEPMVAKPYSPANSVPVSEVEGIEVDQVFIGSC
CCCCCCCCCCCCCCCCCCCCCEECHHHCCHHHCCCCCCCCCCCHHHCCCCEECEEEEECC
TNGRLEDVAVAARILKGKKVAPNTRCIVITASRNVYLQALKKGYVDVLTEAGCLVTFGTC
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCC
GPCVGAHYGVLGPNEVGLFTTNRNFRGRSGHRTSKVYLASPATAAASAVEGKIADPRKYL
CCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHH
KVRVRDMEV
EEEEEECCC
>Mature Secondary Structure 
PSTIAEKILGAKVGKEVSPGELVVVDVDGVMAQDGTAPLAIKVMRERFGEERVFDPEKV
CHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCE
AFVIDHTAPSNNPGTSELHIEMRRFCRKHKCTLYDVGTGICHQVMVEEGRAWPGSVFVGA
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEC
DSHTVTYGALGSFATGVGSTDAAIAMMTGRIWFKVPEALKFNLIGKFREGVMSKDLILKI
CCCEEEEECHHHHHCCCCCCCCEEEEEECEEEEECCCHHEEHHHHHHHHCCCCHHHHHHH
IGDLKEDGATYMSAEFVGDGLKDMRIDSRLTVSNMVVEMGAKVGLMPADEEVMRFVEGRA
HHHHHHCCCCEEEHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RGVPKPELTFPDPGAHYKDEFTYDLSEIEPMVAKPYSPANSVPVSEVEGIEVDQVFIGSC
CCCCCCCCCCCCCCCCCCCCCEECHHHCCHHHCCCCCCCCCCCHHHCCCCEECEEEEECC
TNGRLEDVAVAARILKGKKVAPNTRCIVITASRNVYLQALKKGYVDVLTEAGCLVTFGTC
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCC
GPCVGAHYGVLGPNEVGLFTTNRNFRGRSGHRTSKVYLASPATAAASAVEGKIADPRKYL
CCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHH
KVRVRDMEV
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA