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The map label for this gene is cofH [H]

Identifier: 156937874

GI number: 156937874

Start: 967973

End: 969028

Strand: Reverse

Name: cofH [H]

Synonym: Igni_1086

Alternate gene names: 156937874

Gene position: 969028-967973 (Counterclockwise)

Preceding gene: 156937875

Following gene: 156937873

Centisome position: 74.68

GC content: 57.1

Gene sequence:

>1056_bases
TTGAGCGCGCCGAAGGACCCCCCTAGCTTCTCCGAAGCCTTGGACTTGTGGCACTACGACTTGTACGAGCTAGGCATGAT
GGCCGAAGATCTCCGGAAGAAGACCGTGGGGGACGTGGTTACCTTCGTAGCCAACTACAACATAAACTACACCAACGTCT
GCGTCTACCGCTGCCCCCTGTGCGCTTTTTATAAAGAGAAGGGAGAGGAGGGGGCTTACTTCTTGTCCGTCGAAGAGATG
TTGAAGCAAGCTGCAGAGGCCGTGTGGCTGGGCGCCACGGAGCTCCACGTGGTCGGGGGTATAATTCCGGAACTCACGGT
AGAGTACTTCGAGAAGTTCTTCTCAGAAGTGAAGAGGAGGTGGCCCTTCCTAACCATAAAAGCCTTGACGGCCACCGAGG
TGAGCTACTTGGCCAGGATAAATAGGATGAGCGTGAAGGAAGTGTTAGAGAGGCTGAAGGAGGCCGGGCTAGACGCTATG
CCCGGCGGAGGGGCCGAGGTGTTGGACGACGAGGTCCTCAAGGTAATAGCCCCCGCCAAGAGCGCCGAGGAGTGGCTCAG
GACAATCGAAACCGCCCACGAGCTGGGCATAAGGACCAACGCCACCATGCTGTACGGGCACGTGGAGAGGCCCGAGCACA
TAATTAGACACTTGATGAGGATTAGGGAGCTGCAAGAGAAGACCGGGGGGTTCCTGACCTTCATCCTCTTGAAGTTTAAA
CCCAAGAACACGGAGCTCTGGAGGAAGGGACTGGTTAAGCACGAGGCCCCCGCCCCCTACGACGCCAAGGTAGTAGCCAT
ATCGAGGATAGTGCTGAACGGGTTCATCAACAACATATCCGTGTACTGGGTCGGGTACGGGAAGAAGTACGCCTCCGCGC
TGCTCAACTTCGGCGGCTCCGACTTGGTGGGCACGGCCTTCAGCGAGAAGATATTTAGGTCTGCGGAAGCCCACGACGGC
TACGCTACGCTGGAGGAGTTGGCCCACTACGCTAGGACCTCCGGGAGGGTGCCGGCCCAGAGGGACACGTTCTTTAACAT
AATAAGGTACTTGTAG

Upstream 100 bases:

>100_bases
GCTCGGGGCCGGTCCACCTCCTCACCGCCTCGGACTAGGTCCCGTAACCCTCATCGCTGAAAACCTCTCGCTCGCCCGGG
GGGCTACGGGTCAAAAGGTT

Downstream 100 bases:

>100_bases
GCCCCGTCCCCCCGCCCCCGGAAGCCGAGGTTGCCGGCGGTCGAAGTTAAGGACGTCTGGAGGTCCTTCGGCAAGAAGAC
CGTCCTGAGGGGCGTGAGCT

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSAPKDPPSFSEALDLWHYDLYELGMMAEDLRKKTVGDVVTFVANYNINYTNVCVYRCPLCAFYKEKGEEGAYFLSVEEM
LKQAAEAVWLGATELHVVGGIIPELTVEYFEKFFSEVKRRWPFLTIKALTATEVSYLARINRMSVKEVLERLKEAGLDAM
PGGGAEVLDDEVLKVIAPAKSAEEWLRTIETAHELGIRTNATMLYGHVERPEHIIRHLMRIRELQEKTGGFLTFILLKFK
PKNTELWRKGLVKHEAPAPYDAKVVAISRIVLNGFINNISVYWVGYGKKYASALLNFGGSDLVGTAFSEKIFRSAEAHDG
YATLEELAHYARTSGRVPAQRDTFFNIIRYL

Sequences:

>Translated_351_residues
MSAPKDPPSFSEALDLWHYDLYELGMMAEDLRKKTVGDVVTFVANYNINYTNVCVYRCPLCAFYKEKGEEGAYFLSVEEM
LKQAAEAVWLGATELHVVGGIIPELTVEYFEKFFSEVKRRWPFLTIKALTATEVSYLARINRMSVKEVLERLKEAGLDAM
PGGGAEVLDDEVLKVIAPAKSAEEWLRTIETAHELGIRTNATMLYGHVERPEHIIRHLMRIRELQEKTGGFLTFILLKFK
PKNTELWRKGLVKHEAPAPYDAKVVAISRIVLNGFINNISVYWVGYGKKYASALLNFGGSDLVGTAFSEKIFRSAEAHDG
YATLEELAHYARTSGRVPAQRDTFFNIIRYL
>Mature_350_residues
SAPKDPPSFSEALDLWHYDLYELGMMAEDLRKKTVGDVVTFVANYNINYTNVCVYRCPLCAFYKEKGEEGAYFLSVEEML
KQAAEAVWLGATELHVVGGIIPELTVEYFEKFFSEVKRRWPFLTIKALTATEVSYLARINRMSVKEVLERLKEAGLDAMP
GGGAEVLDDEVLKVIAPAKSAEEWLRTIETAHELGIRTNATMLYGHVERPEHIIRHLMRIRELQEKTGGFLTFILLKFKP
KNTELWRKGLVKHEAPAPYDAKVVAISRIVLNGFINNISVYWVGYGKKYASALLNFGGSDLVGTAFSEKIFRSAEAHDGY
ATLEELAHYARTSGRVPAQRDTFFNIIRYL

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39777; Mature: 39646

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPKDPPSFSEALDLWHYDLYELGMMAEDLRKKTVGDVVTFVANYNINYTNVCVYRCPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
CAFYKEKGEEGAYFLSVEEMLKQAAEAVWLGATELHVVGGIIPELTVEYFEKFFSEVKRR
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPFLTIKALTATEVSYLARINRMSVKEVLERLKEAGLDAMPGGGAEVLDDEVLKVIAPAK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
SAEEWLRTIETAHELGIRTNATMLYGHVERPEHIIRHLMRIRELQEKTGGFLTFILLKFK
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
PKNTELWRKGLVKHEAPAPYDAKVVAISRIVLNGFINNISVYWVGYGKKYASALLNFGGS
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCC
DLVGTAFSEKIFRSAEAHDGYATLEELAHYARTSGRVPAQRDTFFNIIRYL
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SAPKDPPSFSEALDLWHYDLYELGMMAEDLRKKTVGDVVTFVANYNINYTNVCVYRCPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
CAFYKEKGEEGAYFLSVEEMLKQAAEAVWLGATELHVVGGIIPELTVEYFEKFFSEVKRR
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPFLTIKALTATEVSYLARINRMSVKEVLERLKEAGLDAMPGGGAEVLDDEVLKVIAPAK
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
SAEEWLRTIETAHELGIRTNATMLYGHVERPEHIIRHLMRIRELQEKTGGFLTFILLKFK
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
PKNTELWRKGLVKHEAPAPYDAKVVAISRIVLNGFINNISVYWVGYGKKYASALLNFGGS
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHCCCC
DLVGTAFSEKIFRSAEAHDGYATLEELAHYARTSGRVPAQRDTFFNIIRYL
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]