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The map label for this gene is sapA [H]

Identifier: 156937811

GI number: 156937811

Start: 910377

End: 911897

Strand: Reverse

Name: sapA [H]

Synonym: Igni_1021

Alternate gene names: 156937811

Gene position: 911897-910377 (Counterclockwise)

Preceding gene: 156937818

Following gene: 156937810

Centisome position: 70.28

GC content: 60.88

Gene sequence:

>1521_bases
TTGAGGAGTGCATTACTCGTCGCCTTGGCGCTTAGTCTCCAAGCCTCGGCGCTGAGCGTCGGTCTGGGCGACTGGGGGCT
GCCGACGCCCTTCTCCTTCTACCCGAGGGGCCCAGGGTACGTGCTGACGAGCTTCTCCTTCGACACGCTGGTGTGGAAGG
ACGAGAGGGGAGTGATCCCGTGGCTTGCGTACAAGTGGGAGGCGAACGGAACCGCGGTCAAGTTCTACTTGAGGGAGGCC
AAGTGGAGCGACGGGGAGCCCCTCACGGCAGAGGACGTGGTCTTCACGTTCAAGTACCTCATGAAACACGGGTGGCACTG
GAAGAACATCCCTAAGGACCTTATAAAGGACGTCAAGGCTTTGGACGACCGAACGGTGGTCGTAGAGCTCTCGAGGCCTT
TCCCGTTCTTCGTGGAGGAGTACGCGACGACCGTGTTCGTGCTCCCCAAGCACGTGTGGGAGAAGGTGAAGGACCCGTTC
TCCTATAGAGGGAAGGACGCCTTCGTCTGCAGCGGTCCCTACGTGCTGAAGGAATACGTCCCCGGCTCCCGCTACGTCTT
CGTGAGGAACCCCTTCTTCTGGGGACCCAAGCCGAAGTACGAAGAGCTCGTGGTATCTGCCGTAGGCCCCTTCCAGCCTC
AGAAGGCCGTGGCGGCCCTAATAAGGGGGGAGGTAGACAGCGTCTCCCTCTTCGGAAAGGCTTACAGGCTAGTGGAGCTG
GCCAAGAGGGCCAAGCCGGACTTGATCGTAAAGAGGGGGCCCTCCTACTGGGTGCTCTTCTTAGGCTTCAACCTGGACGA
AGAGCCCTACTCCTTCAAGGAGTTTCGCAAGGCCGTCGCAATGTCTTTGGACTTGAAGGAGCTAGTAGAGCTCAGCGCCG
GCCCGGGCGCCGCCGAGCCCGGCTCCCCGGGCTACGTGCCGCCGTACAGCCGCTTCTACGAGGACGACGTGCCTAAGTAC
CGCTACGACCCCGAGGCGGCAGCGAGGCTCCTCAAGTCCGTGGGAGTGGAAGACGTGAACGGGGACGGGTGCGCGGAGTT
CCGAGGGAGGCCGTGGAGGCCGCTGTTAGTAACTACTAAACAGTTCGTGCAAGAAGCCCTAATAGTTAAGAAGATGCTAA
AAAGAGTTAAGATTTGTGTGAACGTCAAGGTGGTTCCCGGGCCCAAGCAGCTCGACTTAATAGTTAAGGGCGGGGGGTAC
GGCTTCGTTATAAGCGGCCACGGGGCGGTGGGGAACGACCCTCTGGCGGCCGCTTGGTGGTTCGAGAGGTTCAGCGCGCC
TTGGAGGGACCCCCGCTACTTGGAGCTCGCGAGGGAGCTCTTGAACGCCCCCAGCGCGGAGGAAGCGATCAAAGTAGTGA
AGGAAATTCAGAGGCTCGTAGCGGAGGAGCTGCCCCGCATAGCGCTTTACTACCCCTACCAGTTCTTGCTCGACTCGTCG
CGGGCCGGGTGGTTCTTCACCGAGGGAGGGATAGACGGCGGCGTGCCGCTGCCCTTCAACAAGCTCGCCCTGTTGAGGTG
A

Upstream 100 bases:

>100_bases
TGTAACACTCGCACAAGACGGCGACCCTCACCTCTCTTCCCCCTCGGGCCGCGCGCGGTAGGGCTATTTACGCGGCACAG
GGCTTGTGCACGGTGACACC

Downstream 100 bases:

>100_bases
GCCGTTGATAGGCAAGGTAATAACGCTAATCATTAGTGTTTTGATAGCGGCGCTCGTGTCCGGTAACCTAGGCAACCCCG
GGGCCGGCCTCGGGAGCTCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRSALLVALALSLQASALSVGLGDWGLPTPFSFYPRGPGYVLTSFSFDTLVWKDERGVIPWLAYKWEANGTAVKFYLREA
KWSDGEPLTAEDVVFTFKYLMKHGWHWKNIPKDLIKDVKALDDRTVVVELSRPFPFFVEEYATTVFVLPKHVWEKVKDPF
SYRGKDAFVCSGPYVLKEYVPGSRYVFVRNPFFWGPKPKYEELVVSAVGPFQPQKAVAALIRGEVDSVSLFGKAYRLVEL
AKRAKPDLIVKRGPSYWVLFLGFNLDEEPYSFKEFRKAVAMSLDLKELVELSAGPGAAEPGSPGYVPPYSRFYEDDVPKY
RYDPEAAARLLKSVGVEDVNGDGCAEFRGRPWRPLLVTTKQFVQEALIVKKMLKRVKICVNVKVVPGPKQLDLIVKGGGY
GFVISGHGAVGNDPLAAAWWFERFSAPWRDPRYLELARELLNAPSAEEAIKVVKEIQRLVAEELPRIALYYPYQFLLDSS
RAGWFFTEGGIDGGVPLPFNKLALLR

Sequences:

>Translated_506_residues
MRSALLVALALSLQASALSVGLGDWGLPTPFSFYPRGPGYVLTSFSFDTLVWKDERGVIPWLAYKWEANGTAVKFYLREA
KWSDGEPLTAEDVVFTFKYLMKHGWHWKNIPKDLIKDVKALDDRTVVVELSRPFPFFVEEYATTVFVLPKHVWEKVKDPF
SYRGKDAFVCSGPYVLKEYVPGSRYVFVRNPFFWGPKPKYEELVVSAVGPFQPQKAVAALIRGEVDSVSLFGKAYRLVEL
AKRAKPDLIVKRGPSYWVLFLGFNLDEEPYSFKEFRKAVAMSLDLKELVELSAGPGAAEPGSPGYVPPYSRFYEDDVPKY
RYDPEAAARLLKSVGVEDVNGDGCAEFRGRPWRPLLVTTKQFVQEALIVKKMLKRVKICVNVKVVPGPKQLDLIVKGGGY
GFVISGHGAVGNDPLAAAWWFERFSAPWRDPRYLELARELLNAPSAEEAIKVVKEIQRLVAEELPRIALYYPYQFLLDSS
RAGWFFTEGGIDGGVPLPFNKLALLR
>Mature_506_residues
MRSALLVALALSLQASALSVGLGDWGLPTPFSFYPRGPGYVLTSFSFDTLVWKDERGVIPWLAYKWEANGTAVKFYLREA
KWSDGEPLTAEDVVFTFKYLMKHGWHWKNIPKDLIKDVKALDDRTVVVELSRPFPFFVEEYATTVFVLPKHVWEKVKDPF
SYRGKDAFVCSGPYVLKEYVPGSRYVFVRNPFFWGPKPKYEELVVSAVGPFQPQKAVAALIRGEVDSVSLFGKAYRLVEL
AKRAKPDLIVKRGPSYWVLFLGFNLDEEPYSFKEFRKAVAMSLDLKELVELSAGPGAAEPGSPGYVPPYSRFYEDDVPKY
RYDPEAAARLLKSVGVEDVNGDGCAEFRGRPWRPLLVTTKQFVQEALIVKKMLKRVKICVNVKVVPGPKQLDLIVKGGGY
GFVISGHGAVGNDPLAAAWWFERFSAPWRDPRYLELARELLNAPSAEEAIKVVKEIQRLVAEELPRIALYYPYQFLLDSS
RAGWFFTEGGIDGGVPLPFNKLALLR

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=443, Percent_Identity=25.9593679458239, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789966, Length=364, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787762, Length=443, Percent_Identity=23.9277652370203, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789887, Length=331, Percent_Identity=26.2839879154079, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787495, Length=453, Percent_Identity=24.9448123620309, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87081878, Length=276, Percent_Identity=25.3623188405797, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57106; Mature: 57106

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSALLVALALSLQASALSVGLGDWGLPTPFSFYPRGPGYVLTSFSFDTLVWKDERGVIP
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCC
WLAYKWEANGTAVKFYLREAKWSDGEPLTAEDVVFTFKYLMKHGWHWKNIPKDLIKDVKA
EEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LDDRTVVVELSRPFPFFVEEYATTVFVLPKHVWEKVKDPFSYRGKDAFVCSGPYVLKEYV
CCCCEEEEEECCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCCEEEEECCCHHHHHCC
PGSRYVFVRNPFFWGPKPKYEELVVSAVGPFQPQKAVAALIRGEVDSVSLFGKAYRLVEL
CCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AKRAKPDLIVKRGPSYWVLFLGFNLDEEPYSFKEFRKAVAMSLDLKELVELSAGPGAAEP
HHHCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GSPGYVPPYSRFYEDDVPKYRYDPEAAARLLKSVGVEDVNGDGCAEFRGRPWRPLLVTTK
CCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHH
QFVQEALIVKKMLKRVKICVNVKVVPGPKQLDLIVKGGGYGFVISGHGAVGNDPLAAAWW
HHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHH
FERFSAPWRDPRYLELARELLNAPSAEEAIKVVKEIQRLVAEELPRIALYYPYQFLLDSS
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHEECCC
RAGWFFTEGGIDGGVPLPFNKLALLR
CCCEEEECCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MRSALLVALALSLQASALSVGLGDWGLPTPFSFYPRGPGYVLTSFSFDTLVWKDERGVIP
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCC
WLAYKWEANGTAVKFYLREAKWSDGEPLTAEDVVFTFKYLMKHGWHWKNIPKDLIKDVKA
EEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LDDRTVVVELSRPFPFFVEEYATTVFVLPKHVWEKVKDPFSYRGKDAFVCSGPYVLKEYV
CCCCEEEEEECCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCCEEEEECCCHHHHHCC
PGSRYVFVRNPFFWGPKPKYEELVVSAVGPFQPQKAVAALIRGEVDSVSLFGKAYRLVEL
CCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AKRAKPDLIVKRGPSYWVLFLGFNLDEEPYSFKEFRKAVAMSLDLKELVELSAGPGAAEP
HHHCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GSPGYVPPYSRFYEDDVPKYRYDPEAAARLLKSVGVEDVNGDGCAEFRGRPWRPLLVTTK
CCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHH
QFVQEALIVKKMLKRVKICVNVKVVPGPKQLDLIVKGGGYGFVISGHGAVGNDPLAAAWW
HHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHH
FERFSAPWRDPRYLELARELLNAPSAEEAIKVVKEIQRLVAEELPRIALYYPYQFLLDSS
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHEECCC
RAGWFFTEGGIDGGVPLPFNKLALLR
CCCEEEECCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]