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The map label for this gene is radA

Identifier: 156937796

GI number: 156937796

Start: 896102

End: 897085

Strand: Reverse

Name: radA

Synonym: Igni_1006

Alternate gene names: 156937796

Gene position: 897085-896102 (Counterclockwise)

Preceding gene: 156937797

Following gene: 156937789

Centisome position: 69.14

GC content: 58.54

Gene sequence:

>984_bases
GTGGGTCAAGTGGCCACTGAGGAGAAGAGGCCGACGTCGGTGGCGGAGCTCCCCGGCGTGGGGCCCTCCACCGCGGCCAA
GCTGATAGACGCCGGCTACGGCACCATAGAGGCGCTGGCCGTCGCCACGCCGGAGGAGCTCGTAGCGATAGGCATACCCC
TCACTACGGCCCAGAAGATCATCAGAGCCGCGAGGCAGATGCTGGACATAAGGTTCAGGACGGCAAAAGAGGTAAAGCTA
GAAAGGATGAACTTAAGGAAGATAACCACCGGCTCTAAGAACTTGGACGACTTGCTGGGAGGCGGTATAGAGACCAAAAC
CATAACCGAATTCTTCGGCGAGTTCGGGAGCGGCAAGAGCCAGCTCTGCCACCAAGCGAGCGTCAACGTCCAACTCCCCT
TGGAGCAAGGGGGCTTGAGCGAGGGCGACAAGGTCGCGAAGGCCGTCTACGTAGACACCGAGGGCACCTTCAGGTGGGAG
AGGATAGAACAAATGGCCAAGTGCTTGGGCCTCGACCCGGACCAAGTAATGGACAACATATACTACATCAGGGCAGTTAA
CAGCGACCACCAGATGGCCATCGTAGAGGAGCTCTTCAACTTGGTACCCAAGGAAAACGTGAAACTAATAGTGGTCGACT
CGGTGACCAGCCACTTCAGGGCGGAGTACCCCGGCCGGGAGAACTTGGCGGTAAGGCAACAGAAGTTGAATAAGCACCTA
CACCAGCTGGGCAAGCTGGCGGAAGTTTACAACACCGCAGTCATAATTACAAACCAAGTAATGGCGAGGCCAGACGTGTT
CTACGGCGACCCCACCCAAGCGGTGGGCGGCCACGTACTCTACCACGCCCCGGGCGTGAGGGTGCAGCTGAAGAAGGCGA
GGGGCAACAAGAGGATAGCGAGGGTTGTAGATGCTCCCCACTTGCCGGAGGCGGAGGCTGTCTTCGCGATAACGGACTGC
GGAATAAGGGACCCGGAGGACTAA

Upstream 100 bases:

>100_bases
CTCGGGAGGCCTAAGTTCGCCAGGGACGCCTACTTCCGAACTTGAGTTACGTCGACCCCTCTTGACTAATAAAGCTCTTT
TTTACCCCAATAGGGCACGG

Downstream 100 bases:

>100_bases
CTAAGTTTTAATAAAAAATCATTTGGAAGCGAACCTCCTCCTCAGCTTGCCTAGGAACGGCAAGGCCAACACTCCGAGGG
CCGGCACCCTGTTAGTCCCG

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MGQVATEEKRPTSVAELPGVGPSTAAKLIDAGYGTIEALAVATPEELVAIGIPLTTAQKIIRAARQMLDIRFRTAKEVKL
ERMNLRKITTGSKNLDDLLGGGIETKTITEFFGEFGSGKSQLCHQASVNVQLPLEQGGLSEGDKVAKAVYVDTEGTFRWE
RIEQMAKCLGLDPDQVMDNIYYIRAVNSDHQMAIVEELFNLVPKENVKLIVVDSVTSHFRAEYPGRENLAVRQQKLNKHL
HQLGKLAEVYNTAVIITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLKKARGNKRIARVVDAPHLPEAEAVFAITDC
GIRDPED

Sequences:

>Translated_327_residues
MGQVATEEKRPTSVAELPGVGPSTAAKLIDAGYGTIEALAVATPEELVAIGIPLTTAQKIIRAARQMLDIRFRTAKEVKL
ERMNLRKITTGSKNLDDLLGGGIETKTITEFFGEFGSGKSQLCHQASVNVQLPLEQGGLSEGDKVAKAVYVDTEGTFRWE
RIEQMAKCLGLDPDQVMDNIYYIRAVNSDHQMAIVEELFNLVPKENVKLIVVDSVTSHFRAEYPGRENLAVRQQKLNKHL
HQLGKLAEVYNTAVIITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLKKARGNKRIARVVDAPHLPEAEAVFAITDC
GIRDPED
>Mature_326_residues
GQVATEEKRPTSVAELPGVGPSTAAKLIDAGYGTIEALAVATPEELVAIGIPLTTAQKIIRAARQMLDIRFRTAKEVKLE
RMNLRKITTGSKNLDDLLGGGIETKTITEFFGEFGSGKSQLCHQASVNVQLPLEQGGLSEGDKVAKAVYVDTEGTFRWER
IEQMAKCLGLDPDQVMDNIYYIRAVNSDHQMAIVEELFNLVPKENVKLIVVDSVTSHFRAEYPGRENLAVRQQKLNKHLH
QLGKLAEVYNTAVIITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLKKARGNKRIARVVDAPHLPEAEAVFAITDCG
IRDPED

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family

Homologues:

Organism=Homo sapiens, GI19924133, Length=322, Percent_Identity=43.7888198757764, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI23238219, Length=315, Percent_Identity=41.2698412698413, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI256017143, Length=324, Percent_Identity=39.8148148148148, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI256017141, Length=324, Percent_Identity=39.8148148148148, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI256017145, Length=240, Percent_Identity=43.3333333333333, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI17402896, Length=343, Percent_Identity=30.0291545189504, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI153946430, Length=266, Percent_Identity=28.1954887218045, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4885659, Length=266, Percent_Identity=28.1954887218045, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI153946427, Length=266, Percent_Identity=28.1954887218045, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI46255039, Length=322, Percent_Identity=24.5341614906832, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI19924117, Length=322, Percent_Identity=24.5341614906832, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI10835029, Length=322, Percent_Identity=24.5341614906832, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71997301, Length=306, Percent_Identity=44.4444444444444, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71997295, Length=306, Percent_Identity=44.4444444444444, Blast_Score=254, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321027, Length=327, Percent_Identity=43.7308868501529, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320942, Length=321, Percent_Identity=42.3676012461059, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6320207, Length=191, Percent_Identity=33.5078534031414, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17864108, Length=324, Percent_Identity=40.7407407407407, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24651285, Length=272, Percent_Identity=41.5441176470588, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17136506, Length=182, Percent_Identity=32.967032967033, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650551, Length=187, Percent_Identity=31.5508021390374, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADA_IGNH4 (A8AB83)

Other databases:

- EMBL:   CP000816
- RefSeq:   YP_001435592.1
- ProteinModelPortal:   A8AB83
- SMR:   A8AB83
- STRING:   A8AB83
- GeneID:   5563025
- GenomeReviews:   CP000816_GR
- KEGG:   iho:Igni_1006
- eggNOG:   arNOG04716
- HOGENOM:   HBG413235
- OMA:   FVARAYN
- ProtClustDB:   PRK04301
- BioCyc:   IHOS453591:IGNI_1006-MONOMER
- HAMAP:   MF_00348
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR020587
- PIRSF:   PIRSF005856
- SMART:   SM00382
- SMART:   SM00278
- TIGRFAMs:   TIGR02236

Pfam domain/function: PF08423 Rad51; SSF47794 Rad51_N

EC number: NA

Molecular weight: Translated: 35907; Mature: 35775

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVATEEKRPTSVAELPGVGPSTAAKLIDAGYGTIEALAVATPEELVAIGIPLTTAQKI
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCHHHHEEECCCHHHEEECCCHHHHHHH
IRAARQMLDIRFRTAKEVKLERMNLRKITTGSKNLDDLLGGGIETKTITEFFGEFGSGKS
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHH
QLCHQASVNVQLPLEQGGLSEGDKVAKAVYVDTEGTFRWERIEQMAKCLGLDPDQVMDNI
HHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCE
YYIRAVNSDHQMAIVEELFNLVPKENVKLIVVDSVTSHFRAEYPGRENLAVRQQKLNKHL
EEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHH
HQLGKLAEVYNTAVIITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLKKARGNKRIA
HHHHHHHHHHCCEEEEECHHHCCCCCEECCCCHHHCCEEEEECCCCEEEEEECCCCCHHH
RVVDAPHLPEAEAVFAITDCGIRDPED
HHHCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
GQVATEEKRPTSVAELPGVGPSTAAKLIDAGYGTIEALAVATPEELVAIGIPLTTAQKI
CCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCHHHHEEECCCHHHEEECCCHHHHHHH
IRAARQMLDIRFRTAKEVKLERMNLRKITTGSKNLDDLLGGGIETKTITEFFGEFGSGKS
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHH
QLCHQASVNVQLPLEQGGLSEGDKVAKAVYVDTEGTFRWERIEQMAKCLGLDPDQVMDNI
HHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCE
YYIRAVNSDHQMAIVEELFNLVPKENVKLIVVDSVTSHFRAEYPGRENLAVRQQKLNKHL
EEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHH
HQLGKLAEVYNTAVIITNQVMARPDVFYGDPTQAVGGHVLYHAPGVRVQLKKARGNKRIA
HHHHHHHHHHCCEEEEECHHHCCCCCEECCCCHHHCCEEEEECCCCEEEEEECCCCCHHH
RVVDAPHLPEAEAVFAITDCGIRDPED
HHHCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA