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The map label for this gene is truB [H]

Identifier: 156937715

GI number: 156937715

Start: 817968

End: 819017

Strand: Direct

Name: truB [H]

Synonym: Igni_0924

Alternate gene names: 156937715

Gene position: 817968-819017 (Clockwise)

Preceding gene: 156937714

Following gene: 156937718

Centisome position: 63.04

GC content: 57.52

Gene sequence:

>1050_bases
GTGAAGGAGGCGCTCGAGTGGTACGAAGAGATGCACCGGAAGTGCGACTTCCGCGGGAAGTGGGTGATTAGGGAGGAGGC
GGAGACCGACCCCAAGTACGGCAAGGAGCCGTGGAAGAGGGACGTGAAGGAGCTGGTGGAGCTCGGCGTGGTGAACTTGG
ACAAGCCGCCCGGCCCGACCTCCCACGAGGTGGTGGCGTGGATAAAGAGGATGTTCGGGTTGGAGAGGGCCGGCCACGGG
GGGACCCTCGACCCCAAGGTAACCGGCGTCCTGCCGGTGGCCCTAGCGGAGGCAACTAAGGTCATCCACTACGTGATGCT
CTCTGGCAAAGAGTACGTGATGGTGATACAGTTCCACGACGCCGTTAAGGAAGAGGAGGTCATCGAGAACTTGAAGTACT
TGGTGGGGGAGATATACCAAAGGCCCCCTCTGAGGAGCAGCGTTAAGAGGCAGTTGAGAACCAAGAAGGTGTATTACATA
CACGTCTTGGAGTTCTGGCCCGAGAGGAGGATGGCCTTAGTGAGGGTCGGCTCGGAGAGCGGAACTTACATGAGAAAGCT
CGCCCACGACTTGGGCTTGCTCGTGGGAACGGGGGCCCACATGAGGGAGCTCAGGAGGACCCGGAGCGGCCCCTTCCACG
AGGATTGGAACTTGGTTCGGATGCAAGACTTAAGCGAGGCTAAATTCTTATACGACAACTACGGCGACGACAGCCTCTTG
AAGAAGTATATAATGCCTTGTGAAGTAGCCACTTGTCATATGCCAAAGATAATGATCAAGGACGGCGCTGTGGACGCGGT
GGCCCACGGCGCCAACGTCTCGATAAGGGGCGTTGCGGCGCTCACCGACAACATGAAGAAGGGGGACGTAGTGGCGGTGG
TTAGCCTAAAGGGGGAGCTAGTGGCAATAGCACAAGCTCTGGTCTCTTCGCAAGAGGCCTTAAAAATGGAGAAGGGTTGG
GTGGCGAAGACCAAGAGAGTGATAATGAAGCCCGGAACGTACCCGGACGTTTGGAGGAAGAAGAAGGCCTCACAGCAAGA
AGGGGGCTAA

Upstream 100 bases:

>100_bases
ATTCCGGACGACAACTACATCTACAAGATAGCGAAAGAAGTCTTCAAAATCCCCGAGGAGAGGCTCCGTAAGGTCCTCAA
GGTCAAGGAGGGGTGACGAG

Downstream 100 bases:

>100_bases
GGCCCTCTTGAGCGCGGCCAGCTTGTGCTCGGTCTCCTCCCACTCCTTTAGGGGTACGGAATAGTAAACTATCCCCGCCC
CGGCTTGGGCCCTCAGCAGC

Product: rRNA pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKEALEWYEEMHRKCDFRGKWVIREEAETDPKYGKEPWKRDVKELVELGVVNLDKPPGPTSHEVVAWIKRMFGLERAGHG
GTLDPKVTGVLPVALAEATKVIHYVMLSGKEYVMVIQFHDAVKEEEVIENLKYLVGEIYQRPPLRSSVKRQLRTKKVYYI
HVLEFWPERRMALVRVGSESGTYMRKLAHDLGLLVGTGAHMRELRRTRSGPFHEDWNLVRMQDLSEAKFLYDNYGDDSLL
KKYIMPCEVATCHMPKIMIKDGAVDAVAHGANVSIRGVAALTDNMKKGDVVAVVSLKGELVAIAQALVSSQEALKMEKGW
VAKTKRVIMKPGTYPDVWRKKKASQQEGG

Sequences:

>Translated_349_residues
MKEALEWYEEMHRKCDFRGKWVIREEAETDPKYGKEPWKRDVKELVELGVVNLDKPPGPTSHEVVAWIKRMFGLERAGHG
GTLDPKVTGVLPVALAEATKVIHYVMLSGKEYVMVIQFHDAVKEEEVIENLKYLVGEIYQRPPLRSSVKRQLRTKKVYYI
HVLEFWPERRMALVRVGSESGTYMRKLAHDLGLLVGTGAHMRELRRTRSGPFHEDWNLVRMQDLSEAKFLYDNYGDDSLL
KKYIMPCEVATCHMPKIMIKDGAVDAVAHGANVSIRGVAALTDNMKKGDVVAVVSLKGELVAIAQALVSSQEALKMEKGW
VAKTKRVIMKPGTYPDVWRKKKASQQEGG
>Mature_349_residues
MKEALEWYEEMHRKCDFRGKWVIREEAETDPKYGKEPWKRDVKELVELGVVNLDKPPGPTSHEVVAWIKRMFGLERAGHG
GTLDPKVTGVLPVALAEATKVIHYVMLSGKEYVMVIQFHDAVKEEEVIENLKYLVGEIYQRPPLRSSVKRQLRTKKVYYI
HVLEFWPERRMALVRVGSESGTYMRKLAHDLGLLVGTGAHMRELRRTRSGPFHEDWNLVRMQDLSEAKFLYDNYGDDSLL
KKYIMPCEVATCHMPKIMIKDGAVDAVAHGANVSIRGVAALTDNMKKGDVVAVVSLKGELVAIAQALVSSQEALKMEKGW
VAKTKRVIMKPGTYPDVWRKKKASQQEGG

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI215599015, Length=330, Percent_Identity=40.9090909090909, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI4503337, Length=316, Percent_Identity=41.7721518987342, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI21040257, Length=254, Percent_Identity=26.3779527559055, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI2367200, Length=190, Percent_Identity=32.1052631578947, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17553978, Length=306, Percent_Identity=39.5424836601307, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6323204, Length=336, Percent_Identity=41.6666666666667, Blast_Score=272, Evalue=5e-74,
Organism=Drosophila melanogaster, GI281364189, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI281364187, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI281364185, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI281364183, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI62471759, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI17975520, Length=320, Percent_Identity=41.5625, Blast_Score=257, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 39644; Mature: 39644

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEALEWYEEMHRKCDFRGKWVIREEAETDPKYGKEPWKRDVKELVELGVVNLDKPPGPT
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCC
SHEVVAWIKRMFGLERAGHGGTLDPKVTGVLPVALAEATKVIHYVMLSGKEYVMVIQFHD
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECH
AVKEEEVIENLKYLVGEIYQRPPLRSSVKRQLRTKKVYYIHVLEFWPERRMALVRVGSES
HCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEEHHCCCCCEEEEEECCCC
GTYMRKLAHDLGLLVGTGAHMRELRRTRSGPFHEDWNLVRMQDLSEAKFLYDNYGDDSLL
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHH
KKYIMPCEVATCHMPKIMIKDGAVDAVAHGANVSIRGVAALTDNMKKGDVVAVVSLKGEL
HHHHCCCCHHHCCCCEEEEECCCHHHHHCCCCCEEEEEHHHHCCCCCCCEEEEEECCCHH
VAIAQALVSSQEALKMEKGWVAKTKRVIMKPGTYPDVWRKKKASQQEGG
HHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKEALEWYEEMHRKCDFRGKWVIREEAETDPKYGKEPWKRDVKELVELGVVNLDKPPGPT
CCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCC
SHEVVAWIKRMFGLERAGHGGTLDPKVTGVLPVALAEATKVIHYVMLSGKEYVMVIQFHD
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECH
AVKEEEVIENLKYLVGEIYQRPPLRSSVKRQLRTKKVYYIHVLEFWPERRMALVRVGSES
HCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEEEHHCCCCCEEEEEECCCC
GTYMRKLAHDLGLLVGTGAHMRELRRTRSGPFHEDWNLVRMQDLSEAKFLYDNYGDDSLL
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHH
KKYIMPCEVATCHMPKIMIKDGAVDAVAHGANVSIRGVAALTDNMKKGDVVAVVSLKGEL
HHHHCCCCHHHCCCCEEEEECCCHHHHHCCCCCEEEEEHHHHCCCCCCCEEEEEECCCHH
VAIAQALVSSQEALKMEKGWVAKTKRVIMKPGTYPDVWRKKKASQQEGG
HHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]