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The map label for this gene is gatA-2 [H]

Identifier: 156937707

GI number: 156937707

Start: 812869

End: 814206

Strand: Direct

Name: gatA-2 [H]

Synonym: Igni_0916

Alternate gene names: 156937707

Gene position: 812869-814206 (Clockwise)

Preceding gene: 156937702

Following gene: 156937709

Centisome position: 62.65

GC content: 61.81

Gene sequence:

>1338_bases
TTGGAGAGCTTGGAGAGGCTTTACGACGCGCTGGAGCGCGACGAGCTCAACGCTTTCATAACCTTGCGCCCCCGCGAGGA
AGTCCTCAGAGAGGCGAGGTCCCTGCTGGAGAGGGGCCTGAGGCCAAAGCCCGTCACGATCAAGGACAACATAAGCACGA
AGGGAATTAGGACCACCGCGGGCTCCTTAATGCTGAAGGATTACGTACCTCCCTTCGACGCGACCGTAATTAAGAGGTTA
AAGGAGAGAGGCTACGTAATAGTAGGAAAGACCAACATGGACGAGTTCGGAATGGGCAGCACCGGTGAGAACAGCGCCTT
CGGGCCCACCAAGAACCCCTTGGACCCGGCCAAAGTGCCGGGGGGCTCTTCCTCGGGGGCCGGCGCGGCCGCTAGGAGGT
ACTCCCTCCCGGGGCTGGGGAGCGACACGGGGGGCTCAGTAAGGGCTCCGGCGGCTTGGTGCTCCGCCTTCGGCTTGAAG
CCCACCTACGGGGCGGTCTCGCGTTACGGGCTTATCCCCTACGCGGACTCGCTCGAGCAGATCTCCCCCATAGGCCCCAC
CGTCAAGAGCGTCGAGGAGCTCTTGGACGTTATAGCCGGTTACGACGAGATGGACGGGACGACCGTGAAACGGGAGCTGC
GTGTGAAGGGCTGTAGCGAGAAGGGGGTGGAGGGCCTAAAGCTCTTCGTCCCGTCTAACGCTCTGGAATACGTCGATAAC
GACGTCAAAGCTGAGTTCAACGCCGCCTTGAGGAAGTTGAGCTCCGAGGGGGCAGAAGTAGAGGAGGGCCAGCTGCCCGA
GCTCGAGGCCGCCTTGCCGGCGTACTACACAATAGCTATGGCGGAGGCGTCCAGCAACCTGTCCCGGTACGACGGCATAA
GGTACGGCCTAAGGGACGAAGACCTCCCGCCCATAACTGAGAACTTCTACGAGTACGTCTCGGAGGTACGCGGGAAGTAC
TTCGGCTGGGAGGTGAAGAGGAGGATAGTCGCCGGCGCGACGGTGCTCTCCGCCGGTTACAGCGAGGAGCTGTACTTGAA
GGCTTTGAAGGCTAGGAGGAAGATAAGCGAACGCATATACGAGATAACCGAGGAAAGGCTCATAGTTACCCCCACCATGC
CGGTCAAGCCGCCTAGCCTGGCCTCGGCCAGGTCGCCCAAGGAGCTCTTCGCAATGGACGTCTTAACGGTCTTCGCCAAC
CTCGCGGGCGTGCCCGCGGGGAGCGCGCCGGTGGGCGAGGGAGTGGGCCTCCAAGTGGTGGGACCGAGGTGGGGCGAGTG
CGAGGTGTTGAGGGTTATGGGGACCTTCGAGAGGAAGGTTGACGCGCCGTGGCTTTAG

Upstream 100 bases:

>100_bases
CAAGCGCGTACCCTCGCGCTAGATGGGTCAAGCGTTTTATTGAGCGGAGCCCTCAAGGCCTGTCAAAAACGGCCCCTCCG
AGCCGGCGCGGGGCCCAACT

Downstream 100 bases:

>100_bases
GCTCTCCACTTAAAGGTCCGGTCACCCCTCCCCGAGTACAGCCCCAGTCATCGCCGCGACACACCTCATAGCCTCCCTTA
AAGCCTTCTTGTGGGCCCTC

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MESLERLYDALERDELNAFITLRPREEVLREARSLLERGLRPKPVTIKDNISTKGIRTTAGSLMLKDYVPPFDATVIKRL
KERGYVIVGKTNMDEFGMGSTGENSAFGPTKNPLDPAKVPGGSSSGAGAAARRYSLPGLGSDTGGSVRAPAAWCSAFGLK
PTYGAVSRYGLIPYADSLEQISPIGPTVKSVEELLDVIAGYDEMDGTTVKRELRVKGCSEKGVEGLKLFVPSNALEYVDN
DVKAEFNAALRKLSSEGAEVEEGQLPELEAALPAYYTIAMAEASSNLSRYDGIRYGLRDEDLPPITENFYEYVSEVRGKY
FGWEVKRRIVAGATVLSAGYSEELYLKALKARRKISERIYEITEERLIVTPTMPVKPPSLASARSPKELFAMDVLTVFAN
LAGVPAGSAPVGEGVGLQVVGPRWGECEVLRVMGTFERKVDAPWL

Sequences:

>Translated_445_residues
MESLERLYDALERDELNAFITLRPREEVLREARSLLERGLRPKPVTIKDNISTKGIRTTAGSLMLKDYVPPFDATVIKRL
KERGYVIVGKTNMDEFGMGSTGENSAFGPTKNPLDPAKVPGGSSSGAGAAARRYSLPGLGSDTGGSVRAPAAWCSAFGLK
PTYGAVSRYGLIPYADSLEQISPIGPTVKSVEELLDVIAGYDEMDGTTVKRELRVKGCSEKGVEGLKLFVPSNALEYVDN
DVKAEFNAALRKLSSEGAEVEEGQLPELEAALPAYYTIAMAEASSNLSRYDGIRYGLRDEDLPPITENFYEYVSEVRGKY
FGWEVKRRIVAGATVLSAGYSEELYLKALKARRKISERIYEITEERLIVTPTMPVKPPSLASARSPKELFAMDVLTVFAN
LAGVPAGSAPVGEGVGLQVVGPRWGECEVLRVMGTFERKVDAPWL
>Mature_445_residues
MESLERLYDALERDELNAFITLRPREEVLREARSLLERGLRPKPVTIKDNISTKGIRTTAGSLMLKDYVPPFDATVIKRL
KERGYVIVGKTNMDEFGMGSTGENSAFGPTKNPLDPAKVPGGSSSGAGAAARRYSLPGLGSDTGGSVRAPAAWCSAFGLK
PTYGAVSRYGLIPYADSLEQISPIGPTVKSVEELLDVIAGYDEMDGTTVKRELRVKGCSEKGVEGLKLFVPSNALEYVDN
DVKAEFNAALRKLSSEGAEVEEGQLPELEAALPAYYTIAMAEASSNLSRYDGIRYGLRDEDLPPITENFYEYVSEVRGKY
FGWEVKRRIVAGATVLSAGYSEELYLKALKARRKISERIYEITEERLIVTPTMPVKPPSLASARSPKELFAMDVLTVFAN
LAGVPAGSAPVGEGVGLQVVGPRWGECEVLRVMGTFERKVDAPWL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=474, Percent_Identity=34.1772151898734, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI195972892, Length=423, Percent_Identity=26.241134751773, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=36.8055555555556, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17537465, Length=448, Percent_Identity=24.3303571428571, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=120, Percent_Identity=35, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=118, Percent_Identity=35.5932203389831, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=120, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=457, Percent_Identity=36.5426695842451, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=443, Percent_Identity=28.2167042889391, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=155, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=472, Percent_Identity=35.3813559322034, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161078093, Length=462, Percent_Identity=29.2207792207792, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24644968, Length=417, Percent_Identity=26.1390887290168, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652985, Length=400, Percent_Identity=26.25, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19922090, Length=400, Percent_Identity=26.25, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24652981, Length=400, Percent_Identity=26.25, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24652983, Length=400, Percent_Identity=26.25, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45550774, Length=305, Percent_Identity=27.8688524590164, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648435, Length=305, Percent_Identity=27.8688524590164, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648437, Length=305, Percent_Identity=27.8688524590164, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=304, Percent_Identity=27.9605263157895, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=304, Percent_Identity=27.9605263157895, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356731, Length=157, Percent_Identity=34.3949044585987, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48494; Mature: 48494

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLERLYDALERDELNAFITLRPREEVLREARSLLERGLRPKPVTIKDNISTKGIRTTA
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHH
GSLMLKDYVPPFDATVIKRLKERGYVIVGKTNMDEFGMGSTGENSAFGPTKNPLDPAKVP
HHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGSSSGAGAAARRYSLPGLGSDTGGSVRAPAAWCSAFGLKPTYGAVSRYGLIPYADSLEQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCHHHHHH
ISPIGPTVKSVEELLDVIAGYDEMDGTTVKRELRVKGCSEKGVEGLKLFVPSNALEYVDN
HCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCC
DVKAEFNAALRKLSSEGAEVEEGQLPELEAALPAYYTIAMAEASSNLSRYDGIRYGLRDE
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHEEHHHHHCCHHHHCCCCCCCCCC
DLPPITENFYEYVSEVRGKYFGWEVKRRIVAGATVLSAGYSEELYLKALKARRKISERIY
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EITEERLIVTPTMPVKPPSLASARSPKELFAMDVLTVFANLAGVPAGSAPVGEGVGLQVV
HHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
GPRWGECEVLRVMGTFERKVDAPWL
CCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MESLERLYDALERDELNAFITLRPREEVLREARSLLERGLRPKPVTIKDNISTKGIRTTA
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHH
GSLMLKDYVPPFDATVIKRLKERGYVIVGKTNMDEFGMGSTGENSAFGPTKNPLDPAKVP
HHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGSSSGAGAAARRYSLPGLGSDTGGSVRAPAAWCSAFGLKPTYGAVSRYGLIPYADSLEQ
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCHHHHHH
ISPIGPTVKSVEELLDVIAGYDEMDGTTVKRELRVKGCSEKGVEGLKLFVPSNALEYVDN
HCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCC
DVKAEFNAALRKLSSEGAEVEEGQLPELEAALPAYYTIAMAEASSNLSRYDGIRYGLRDE
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHEEHHHHHCCHHHHCCCCCCCCCC
DLPPITENFYEYVSEVRGKYFGWEVKRRIVAGATVLSAGYSEELYLKALKARRKISERIY
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EITEERLIVTPTMPVKPPSLASARSPKELFAMDVLTVFANLAGVPAGSAPVGEGVGLQVV
HHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
GPRWGECEVLRVMGTFERKVDAPWL
CCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]