Click here to switch to the map view.

The map label for this gene is fabH [C]

Identifier: 156937170

GI number: 156937170

Start: 331246

End: 332289

Strand: Direct

Name: fabH [C]

Synonym: Igni_0376

Alternate gene names: 156937170

Gene position: 331246-332289 (Clockwise)

Preceding gene: 156937169

Following gene: 156937171

Centisome position: 25.53

GC content: 61.78

Gene sequence:

>1044_bases
TTGGTCGGCATAGCGGGCTGGGGGGCCTACGTGCCCCTCTACAGAGTCTCCACAGAAGAGATAGCGCTTCACTGGGGCTC
CGACCCTAAGGTCCCCTTATCCTTGGGCGTGAAGGAGAAGTCCGTCGCGGGGCCTGACGAGGACTCGGTAACCATAGCGT
GGGAGGCCGCGGTAAACGCCTTGACGAGGGCCCAAGTGGACCCTAAAGACGTAGGGATGGTCTTCATAGGCTCCGAGTCC
AAGCCCTACGCGGTCAAGCCCTCCTCCACGATAATTGCCGACGCCCTAGGGATAGCTCCAGAGACCATGGCAGTGGACGC
GGAGTTCGCTTGCAGGGCAGCAAGCGAGGGCTTGCGCCACGCGCTGGTCTTGGTGAGCGAGGGGGCGGTGAGGCACGCGT
TGATAATAGGGACCGACACCGCCCAAGGGGCGCCCGGGGACGTCTTGGAGTACACGGCCGCCTCCGGCGGGGCGGCCTTC
GTGGTCTCCAACGAAAACGTCGTCGCCAACTTCCTCGACGCCTACACCTTCGTTACCGATACCCCGGACTTCTGGAGGCG
CGACGGGAGCCAGTTCCCCGCCCACGGGGAGGGCTTCACCGGGGAGCCGGCCTACTTCTACCACTTGTACAACGCCATAA
AAGAGCTGATGGAGAGGAACTCGTTAAAGCCCTCGGACGTGGCCTACTTCGTCCCCCACCAGCCCAACGTGAGGTTCCCC
ATGAAGCTGGCGAAGATGTTGGGCTTCAAGAGGGAACAGCTAATGATAGGCTTGACCACAGCTGACATAGGGAACACCTA
CAACGCCTCTGCCTTCCTAGGGATGACGAGGGTACTCGACAACGCGTCGCCGGGCGACTTGGTGCTGATGGCGCCGTTCG
GCTCGGGGGCGGGGGCGGACGCTTACCTCTTCGAGGTTACGGACAGGGTGGAGGAGGCCAAGGCCTTGGCGCCCACCACG
CAGAGCTACATAGAGAGGAGGAAGGTTATAGACTACGCGACCTACTTGCGGTTCAGGAACAAGATAAAGAAGTTGGCGAG
GTGA

Upstream 100 bases:

>100_bases
GTTTTCAAAGGCGTTTAACGAGGTTTGGCGGTAGTCGAACATAATATCAGACCAGCGTCTTAACTCCGCCTCCCCTTAGA
GGGCGCGGAGCGGTGAAACC

Downstream 100 bases:

>100_bases
GGCCGTTGTACGTCTGCTCGGCCAACGTGGTGAAGGTCGATAGGCACTACGACAAGAGCTCTTCTGCCTTGGCCGTGGAG
GCCCTCGCGCCGGTGGTGGA

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MVGIAGWGAYVPLYRVSTEEIALHWGSDPKVPLSLGVKEKSVAGPDEDSVTIAWEAAVNALTRAQVDPKDVGMVFIGSES
KPYAVKPSSTIIADALGIAPETMAVDAEFACRAASEGLRHALVLVSEGAVRHALIIGTDTAQGAPGDVLEYTAASGGAAF
VVSNENVVANFLDAYTFVTDTPDFWRRDGSQFPAHGEGFTGEPAYFYHLYNAIKELMERNSLKPSDVAYFVPHQPNVRFP
MKLAKMLGFKREQLMIGLTTADIGNTYNASAFLGMTRVLDNASPGDLVLMAPFGSGAGADAYLFEVTDRVEEAKALAPTT
QSYIERRKVIDYATYLRFRNKIKKLAR

Sequences:

>Translated_347_residues
MVGIAGWGAYVPLYRVSTEEIALHWGSDPKVPLSLGVKEKSVAGPDEDSVTIAWEAAVNALTRAQVDPKDVGMVFIGSES
KPYAVKPSSTIIADALGIAPETMAVDAEFACRAASEGLRHALVLVSEGAVRHALIIGTDTAQGAPGDVLEYTAASGGAAF
VVSNENVVANFLDAYTFVTDTPDFWRRDGSQFPAHGEGFTGEPAYFYHLYNAIKELMERNSLKPSDVAYFVPHQPNVRFP
MKLAKMLGFKREQLMIGLTTADIGNTYNASAFLGMTRVLDNASPGDLVLMAPFGSGAGADAYLFEVTDRVEEAKALAPTT
QSYIERRKVIDYATYLRFRNKIKKLAR
>Mature_347_residues
MVGIAGWGAYVPLYRVSTEEIALHWGSDPKVPLSLGVKEKSVAGPDEDSVTIAWEAAVNALTRAQVDPKDVGMVFIGSES
KPYAVKPSSTIIADALGIAPETMAVDAEFACRAASEGLRHALVLVSEGAVRHALIIGTDTAQGAPGDVLEYTAASGGAAF
VVSNENVVANFLDAYTFVTDTPDFWRRDGSQFPAHGEGFTGEPAYFYHLYNAIKELMERNSLKPSDVAYFVPHQPNVRFP
MKLAKMLGFKREQLMIGLTTADIGNTYNASAFLGMTRVLDNASPGDLVLMAPFGSGAGADAYLFEVTDRVEEAKALAPTT
QSYIERRKVIDYATYLRFRNKIKKLAR

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

Organism=Escherichia coli, GI1787333, Length=310, Percent_Identity=26.4516129032258, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08541 ACP_syn_III_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 37526; Mature: 37526

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGIAGWGAYVPLYRVSTEEIALHWGSDPKVPLSLGVKEKSVAGPDEDSVTIAWEAAVNA
CEEECCCCCCCEEEEECHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHHHH
LTRAQVDPKDVGMVFIGSESKPYAVKPSSTIIADALGIAPETMAVDAEFACRAASEGLRH
HHHCCCCHHHCCEEEECCCCCCEEECCCCHHHHHHHCCCCCCEEECHHHHHHHHHHHHHE
ALVLVSEGAVRHALIIGTDTAQGAPGDVLEYTAASGGAAFVVSNENVVANFLDAYTFVTD
EEEEEECCCEEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHEECC
TPDFWRRDGSQFPAHGEGFTGEPAYFYHLYNAIKELMERNSLKPSDVAYFVPHQPNVRFP
CHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHH
MKLAKMLGFKREQLMIGLTTADIGNTYNASAFLGMTRVLDNASPGDLVLMAPFGSGAGAD
HHHHHHHCCCCHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
AYLFEVTDRVEEAKALAPTTQSYIERRKVIDYATYLRFRNKIKKLAR
EEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVGIAGWGAYVPLYRVSTEEIALHWGSDPKVPLSLGVKEKSVAGPDEDSVTIAWEAAVNA
CEEECCCCCCCEEEEECHHHEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHHHH
LTRAQVDPKDVGMVFIGSESKPYAVKPSSTIIADALGIAPETMAVDAEFACRAASEGLRH
HHHCCCCHHHCCEEEECCCCCCEEECCCCHHHHHHHCCCCCCEEECHHHHHHHHHHHHHE
ALVLVSEGAVRHALIIGTDTAQGAPGDVLEYTAASGGAAFVVSNENVVANFLDAYTFVTD
EEEEEECCCEEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHEECC
TPDFWRRDGSQFPAHGEGFTGEPAYFYHLYNAIKELMERNSLKPSDVAYFVPHQPNVRFP
CHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHH
MKLAKMLGFKREQLMIGLTTADIGNTYNASAFLGMTRVLDNASPGDLVLMAPFGSGAGAD
HHHHHHHCCCCHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
AYLFEVTDRVEEAKALAPTTQSYIERRKVIDYATYLRFRNKIKKLAR
EEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]