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The map label for this gene is rpoA2 [H]

Identifier: 156937039

GI number: 156937039

Start: 212753

End: 214021

Strand: Direct

Name: rpoA2 [H]

Synonym: Igni_0244

Alternate gene names: 156937039

Gene position: 212753-214021 (Clockwise)

Preceding gene: 156937038

Following gene: 156937040

Centisome position: 16.4

GC content: 54.85

Gene sequence:

>1269_bases
GTGAGCGGGGCGGCCGAGAAGCTGGAGTACGCGTTCAAGCTGATAGACGAGTGGGAGAGGAAGCTTTACGAGGAGTACAA
CATAAGGTCGAAGCCGAAGTTCATCGAAGAGCTGAGGAGGAACATCGAGAAGCTGATAGACGTAAAGCCGGAGGTCAGCG
AGGAAGAGCTGAAGAAGGTAGTGGACCTGACGTTCAGGTACTTCCTGAGGGCGCAGGTCGAGCCCGGAGAGGCCGTGGGC
ACGGTGGCTGCCCAGTCCATAGGCGAGCCCTCCACGCAGATGACACTGAGGACCTTCCACTATGCGGGCGTAAAGGAGCT
AAACGTAACCTTGGGCCTTCCTAGGCTAATAGAGATAGTTGACGCGAGGCGCACCCCCAGCACCCCCACTATGACCGTCT
ACTTGGACGAGGAACACAGACACGACAAAGAGAAAGCTCTCGAAGTGGCTAGAAAGATAGAGATGACGAAGGTCGAAAAC
GTGGCGTCGAGCGTAGAGATAGATTGGTTCGAGACCACCATAATTGTTCGGCTCGACCCAGAGATGATGGAGGATAAAGG
AATAACGAGGGACGACGTGATCAAGGCAATAAACAGGTTGAGGATAAAGGGAAGGGCGGAGCCGGACCCCGACGACGAAT
ACGCCGTGCTCGTGAGGCTGGACCCGGAGGCTTACACCTCCATAGGCGAGCTGCAGAAGGTAAGGGATAAGGTTCTGTCC
GCTAAGATTAAGGGCATTAAAGGGATAAACAGAGTAATCGTTCAACAGAGGGAGGATGAGTACGTCTTAGTTACCGACGG
CTCTAACTTGGCCCAAGTGTTAGAGGTAGAAGGCGTGGACCCCACTCGGACGGTGAGCGACAGCATCAAGGAGATAGAAG
AAGTGTTAGGTATAGAAGCCGCTAGGCAAGCAATAATAAATGAGATATTGGACGTCTTGGAGAACCAAGGTTTGGACGTG
GATAGGAGGCACATAATGTTGTTAGCCGACGCGATGACTTGGAGCGGCGAGGTCAGGCAGGTGGGGAGGCACGGGATAGC
CGGTACCAAGCCCAGCGTGCTCGCCAGGGCGGCCTTCGAGGTCACTGTTAAGCAGCTCGTGGACGCGGCGGTGGAAGGGG
AGGTGGACGAGCTGAAGGGAGTCACTGAGAACATAATAATCGGCCAGCCGATACCGTTGGGCACCGGGATGGTGCTCTTG
GCTATGAATCCGCAAGAGTTCGTGAGGAGAGTTAAAAAGAGCAGTGAAGAGCGCCAAGGGGGTGAATGA

Upstream 100 bases:

>100_bases
AGAGCGTCTACGGCGACGACGGCGTGGACCCCATGAAGAGTGCCCACGGGGAGTCGGTCGACGTGAAGCACGTAATTGAA
AAGGTCACGAAGGTGAGACT

Downstream 100 bases:

>100_bases
CCGTGACCGCGATAGAGGCCGAGTTGAAGACTGCCTTGAGAACGGGAAAAGTGGTCTTGGGCGCTAAACAAACCATAAAG
TTGTTGAAGACCGGGAAGGC

Product: DNA-directed RNA polymerase, subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSGAAEKLEYAFKLIDEWERKLYEEYNIRSKPKFIEELRRNIEKLIDVKPEVSEEELKKVVDLTFRYFLRAQVEPGEAVG
TVAAQSIGEPSTQMTLRTFHYAGVKELNVTLGLPRLIEIVDARRTPSTPTMTVYLDEEHRHDKEKALEVARKIEMTKVEN
VASSVEIDWFETTIIVRLDPEMMEDKGITRDDVIKAINRLRIKGRAEPDPDDEYAVLVRLDPEAYTSIGELQKVRDKVLS
AKIKGIKGINRVIVQQREDEYVLVTDGSNLAQVLEVEGVDPTRTVSDSIKEIEEVLGIEAARQAIINEILDVLENQGLDV
DRRHIMLLADAMTWSGEVRQVGRHGIAGTKPSVLARAAFEVTVKQLVDAAVEGEVDELKGVTENIIIGQPIPLGTGMVLL
AMNPQEFVRRVKKSSEERQGGE

Sequences:

>Translated_422_residues
MSGAAEKLEYAFKLIDEWERKLYEEYNIRSKPKFIEELRRNIEKLIDVKPEVSEEELKKVVDLTFRYFLRAQVEPGEAVG
TVAAQSIGEPSTQMTLRTFHYAGVKELNVTLGLPRLIEIVDARRTPSTPTMTVYLDEEHRHDKEKALEVARKIEMTKVEN
VASSVEIDWFETTIIVRLDPEMMEDKGITRDDVIKAINRLRIKGRAEPDPDDEYAVLVRLDPEAYTSIGELQKVRDKVLS
AKIKGIKGINRVIVQQREDEYVLVTDGSNLAQVLEVEGVDPTRTVSDSIKEIEEVLGIEAARQAIINEILDVLENQGLDV
DRRHIMLLADAMTWSGEVRQVGRHGIAGTKPSVLARAAFEVTVKQLVDAAVEGEVDELKGVTENIIIGQPIPLGTGMVLL
AMNPQEFVRRVKKSSEERQGGE
>Mature_421_residues
SGAAEKLEYAFKLIDEWERKLYEEYNIRSKPKFIEELRRNIEKLIDVKPEVSEEELKKVVDLTFRYFLRAQVEPGEAVGT
VAAQSIGEPSTQMTLRTFHYAGVKELNVTLGLPRLIEIVDARRTPSTPTMTVYLDEEHRHDKEKALEVARKIEMTKVENV
ASSVEIDWFETTIIVRLDPEMMEDKGITRDDVIKAINRLRIKGRAEPDPDDEYAVLVRLDPEAYTSIGELQKVRDKVLSA
KIKGIKGINRVIVQQREDEYVLVTDGSNLAQVLEVEGVDPTRTVSDSIKEIEEVLGIEAARQAIINEILDVLENQGLDVD
RRHIMLLADAMTWSGEVRQVGRHGIAGTKPSVLARAAFEVTVKQLVDAAVEGEVDELKGVTENIIIGQPIPLGTGMVLLA
MNPQEFVRRVKKSSEERQGGE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=410, Percent_Identity=35.8536585365854, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI4505939, Length=405, Percent_Identity=33.8271604938272, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI103471997, Length=86, Percent_Identity=55.8139534883721, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25145495, Length=371, Percent_Identity=35.3099730458221, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71987878, Length=408, Percent_Identity=34.3137254901961, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71998295, Length=82, Percent_Identity=53.6585365853659, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320061, Length=400, Percent_Identity=35, Blast_Score=215, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6324690, Length=369, Percent_Identity=36.5853658536585, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324917, Length=91, Percent_Identity=49.4505494505495, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281360912, Length=395, Percent_Identity=38.2278481012658, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17530899, Length=432, Percent_Identity=33.1018518518519, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17647875, Length=165, Percent_Identity=32.7272727272727, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 47552; Mature: 47421

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAAEKLEYAFKLIDEWERKLYEEYNIRSKPKFIEELRRNIEKLIDVKPEVSEEELKKV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VDLTFRYFLRAQVEPGEAVGTVAAQSIGEPSTQMTLRTFHYAGVKELNVTLGLPRLIEIV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEECCHHHHHHH
DARRTPSTPTMTVYLDEEHRHDKEKALEVARKIEMTKVENVASSVEIDWFETTIIVRLDP
HCCCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEECH
EMMEDKGITRDDVIKAINRLRIKGRAEPDPDDEYAVLVRLDPEAYTSIGELQKVRDKVLS
HHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
AKIKGIKGINRVIVQQREDEYVLVTDGSNLAQVLEVEGVDPTRTVSDSIKEIEEVLGIEA
HHHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCHHH
ARQAIINEILDVLENQGLDVDRRHIMLLADAMTWSGEVRQVGRHGIAGTKPSVLARAAFE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VTVKQLVDAAVEGEVDELKGVTENIIIGQPIPLGTGMVLLAMNPQEFVRRVKKSSEERQG
HHHHHHHHHHHCCCHHHHCCCHHCEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHCCC
GE
CC
>Mature Secondary Structure 
SGAAEKLEYAFKLIDEWERKLYEEYNIRSKPKFIEELRRNIEKLIDVKPEVSEEELKKV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
VDLTFRYFLRAQVEPGEAVGTVAAQSIGEPSTQMTLRTFHYAGVKELNVTLGLPRLIEIV
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEECCHHHHHHH
DARRTPSTPTMTVYLDEEHRHDKEKALEVARKIEMTKVENVASSVEIDWFETTIIVRLDP
HCCCCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEEECH
EMMEDKGITRDDVIKAINRLRIKGRAEPDPDDEYAVLVRLDPEAYTSIGELQKVRDKVLS
HHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
AKIKGIKGINRVIVQQREDEYVLVTDGSNLAQVLEVEGVDPTRTVSDSIKEIEEVLGIEA
HHHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHCHHH
ARQAIINEILDVLENQGLDVDRRHIMLLADAMTWSGEVRQVGRHGIAGTKPSVLARAAFE
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
VTVKQLVDAAVEGEVDELKGVTENIIIGQPIPLGTGMVLLAMNPQEFVRRVKKSSEERQG
HHHHHHHHHHHCCCHHHHCCCHHCEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA