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The map label for this gene is gatD [H]

Identifier: 156936996

GI number: 156936996

Start: 178708

End: 180036

Strand: Direct

Name: gatD [H]

Synonym: Igni_0201

Alternate gene names: 156936996

Gene position: 178708-180036 (Clockwise)

Preceding gene: 156936993

Following gene: 156936998

Centisome position: 13.77

GC content: 58.99

Gene sequence:

>1329_bases
TTGAGTTACCCGTCCGACTTGGCGGCCTCGCTTCTCCAGAAGGGCATAGAAGTAGGGGACGTGATTAAGGTAGTGAGGGA
TGACGGCTTAGTAGTCGAAGGCATACTCATGCCCAGGCCGAAGGGCGGAAGGTACATAGTCGTCAAGCTGAAGAACGGTT
ACAACGTCGGGATAGACGTAAGGAGGATAAAGGAATTAGAGCTCGTGGCTAAGAGGAAGCCCCAACGGAGGGAGAAGGGC
GAGAAGCCCCCTAGGAGGCCGGGCTACCCTTTGGTAAAGGTGTTAGGCACGGGCGGTACCATAGCTTCTAGGATAGACTA
CGAAACGGGCGCCGTGAAGCCAGCCATGAGTACGGAAGAGCTCGTAGAGGAGGTACCGGAGTTGCTCGACTACGCCGACG
TGGAGGCTGAACAGATAATGGACGTCCTCTCGGAGAACATGACTCCGGAGATGTGGAGCCGCGTGGCGGAAGCGGTACAC
GCGGAGCTGCTGAGGGAGGAGGTTGCTGGAGTAGTGGTGGCCCACGGGACTGACACGATGGTTTACACCGCCTCCGCGGT
AGCGTTCGCAATAAGAGAGATAAACAAACCCGTCGTGTTCGTGGGGGCGCAGAGGAGCTCCGACAGGCCTTCCAGCGACG
CTACGCTGAACCTCATAGGGGCTACTATAGCGGCCGCGAGGTCTGACATAGCGGAGTCCATAATAGCGATGCACGGGGAA
AGCAGCGACACCTTCGTGGTCTTGCACAGAGCCACGAGGACTAAGAAGTTCCACTCGACGCGCAGGGACGCGTTTCAGAG
CATATGTGCCCAGCCCCTAGCTTACGTGTGGCCGTGGACCGGCGACGTCGAACCGAAGTCCCGGCCGCTGCGCACGAGGG
GCGACTGGACCCCGAAGCTGATGAACTCCTTCGAGCCGCGGGTGCTCCAGCTAAGGGCTTACCCCGGGATGCCCCCGGAC
CTCCTATACTTGGCCAAGGATAAGGGCTTCAAAGGGGTGATAATAGAGGGCACCGGAATGGGCCACGTCCCGGAGTACTT
GGTGGACGCCATAAAGGAGTTGACCGAGTCCGGAATATTCGTCGGCGTGACGACCCAGTGCGTCCACGGCACCACAAACT
TGAACGTCTACGCGACCGGGAGGAAGATGCTGGAGGCCGGGGCGACCCCCTTAGGGGACATGTTAACTGAAGTGGCAACG
GTCAAGCTTATGTGGGCGTTGGCCAACTATCCCCCGGAGGAGGTACCCAAAGTCATGGTGACCCCTCTAGAGGGCGAGTT
GAGGGAGCGCAGGTTGCTCAGAGATTATCGGCTCTCGTATAGGCGATGA

Upstream 100 bases:

>100_bases
CCGCCGATGGCGGGCGGTGAGGGACCCCGGCACCCCTGGGCACCTCCCCCCTCACATAAGATTAATGCTCAACCTCGCCT
CTTTCCGGGGCGCGCAGCAG

Downstream 100 bases:

>100_bases
CCTTCCTTAACGAGGCCACGAAGCTCAAGGGGGCTACGAGGGGGTCGTAGGGCAAGCTCACTAGGCCGGCCCTCATCCTA
ACCGCCATCCCCCTCACCCA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSYPSDLAASLLQKGIEVGDVIKVVRDDGLVVEGILMPRPKGGRYIVVKLKNGYNVGIDVRRIKELELVAKRKPQRREKG
EKPPRRPGYPLVKVLGTGGTIASRIDYETGAVKPAMSTEELVEEVPELLDYADVEAEQIMDVLSENMTPEMWSRVAEAVH
AELLREEVAGVVVAHGTDTMVYTASAVAFAIREINKPVVFVGAQRSSDRPSSDATLNLIGATIAAARSDIAESIIAMHGE
SSDTFVVLHRATRTKKFHSTRRDAFQSICAQPLAYVWPWTGDVEPKSRPLRTRGDWTPKLMNSFEPRVLQLRAYPGMPPD
LLYLAKDKGFKGVIIEGTGMGHVPEYLVDAIKELTESGIFVGVTTQCVHGTTNLNVYATGRKMLEAGATPLGDMLTEVAT
VKLMWALANYPPEEVPKVMVTPLEGELRERRLLRDYRLSYRR

Sequences:

>Translated_442_residues
MSYPSDLAASLLQKGIEVGDVIKVVRDDGLVVEGILMPRPKGGRYIVVKLKNGYNVGIDVRRIKELELVAKRKPQRREKG
EKPPRRPGYPLVKVLGTGGTIASRIDYETGAVKPAMSTEELVEEVPELLDYADVEAEQIMDVLSENMTPEMWSRVAEAVH
AELLREEVAGVVVAHGTDTMVYTASAVAFAIREINKPVVFVGAQRSSDRPSSDATLNLIGATIAAARSDIAESIIAMHGE
SSDTFVVLHRATRTKKFHSTRRDAFQSICAQPLAYVWPWTGDVEPKSRPLRTRGDWTPKLMNSFEPRVLQLRAYPGMPPD
LLYLAKDKGFKGVIIEGTGMGHVPEYLVDAIKELTESGIFVGVTTQCVHGTTNLNVYATGRKMLEAGATPLGDMLTEVAT
VKLMWALANYPPEEVPKVMVTPLEGELRERRLLRDYRLSYRR
>Mature_441_residues
SYPSDLAASLLQKGIEVGDVIKVVRDDGLVVEGILMPRPKGGRYIVVKLKNGYNVGIDVRRIKELELVAKRKPQRREKGE
KPPRRPGYPLVKVLGTGGTIASRIDYETGAVKPAMSTEELVEEVPELLDYADVEAEQIMDVLSENMTPEMWSRVAEAVHA
ELLREEVAGVVVAHGTDTMVYTASAVAFAIREINKPVVFVGAQRSSDRPSSDATLNLIGATIAAARSDIAESIIAMHGES
SDTFVVLHRATRTKKFHSTRRDAFQSICAQPLAYVWPWTGDVEPKSRPLRTRGDWTPKLMNSFEPRVLQLRAYPGMPPDL
LYLAKDKGFKGVIIEGTGMGHVPEYLVDAIKELTESGIFVGVTTQCVHGTTNLNVYATGRKMLEAGATPLGDMLTEVATV
KLMWALANYPPEEVPKVMVTPLEGELRERRLLRDYRLSYRR

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=343, Percent_Identity=29.1545189504373, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788065, Length=306, Percent_Identity=31.0457516339869, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789327, Length=280, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI193207372, Length=282, Percent_Identity=30.8510638297872, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17558280, Length=282, Percent_Identity=30.8510638297872, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17558278, Length=282, Percent_Identity=30.8510638297872, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193207374, Length=282, Percent_Identity=30.8510638297872, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320527, Length=274, Percent_Identity=26.2773722627737, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323189, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323187, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323186, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323184, Length=267, Percent_Identity=29.9625468164794, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24645530, Length=320, Percent_Identity=30.625, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24639609, Length=331, Percent_Identity=28.3987915407855, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: 3.5.1.1

Molecular weight: Translated: 48897; Mature: 48766

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPSDLAASLLQKGIEVGDVIKVVRDDGLVVEGILMPRPKGGRYIVVKLKNGYNVGIDV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCH
RRIKELELVAKRKPQRREKGEKPPRRPGYPLVKVLGTGGTIASRIDYETGAVKPAMSTEE
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHH
LVEEVPELLDYADVEAEQIMDVLSENMTPEMWSRVAEAVHAELLREEVAGVVVAHGTDTM
HHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCE
VYTASAVAFAIREINKPVVFVGAQRSSDRPSSDATLNLIGATIAAARSDIAESIIAMHGE
EEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
SSDTFVVLHRATRTKKFHSTRRDAFQSICAQPLAYVWPWTGDVEPKSRPLRTRGDWTPKL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCCHHH
MNSFEPRVLQLRAYPGMPPDLLYLAKDKGFKGVIIEGTGMGHVPEYLVDAIKELTESGIF
HCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEE
VGVTTQCVHGTTNLNVYATGRKMLEAGATPLGDMLTEVATVKLMWALANYPPEEVPKVMV
EEEEHHEECCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
TPLEGELRERRLLRDYRLSYRR
ECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYPSDLAASLLQKGIEVGDVIKVVRDDGLVVEGILMPRPKGGRYIVVKLKNGYNVGIDV
CCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCH
RRIKELELVAKRKPQRREKGEKPPRRPGYPLVKVLGTGGTIASRIDYETGAVKPAMSTEE
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCHHH
LVEEVPELLDYADVEAEQIMDVLSENMTPEMWSRVAEAVHAELLREEVAGVVVAHGTDTM
HHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCE
VYTASAVAFAIREINKPVVFVGAQRSSDRPSSDATLNLIGATIAAARSDIAESIIAMHGE
EEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCC
SSDTFVVLHRATRTKKFHSTRRDAFQSICAQPLAYVWPWTGDVEPKSRPLRTRGDWTPKL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCCCCCHHH
MNSFEPRVLQLRAYPGMPPDLLYLAKDKGFKGVIIEGTGMGHVPEYLVDAIKELTESGIF
HCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEE
VGVTTQCVHGTTNLNVYATGRKMLEAGATPLGDMLTEVATVKLMWALANYPPEEVPKVMV
EEEEHHEECCCCCEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
TPLEGELRERRLLRDYRLSYRR
ECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA