Click here to switch to the map view.

The map label for this gene is polB [H]

Identifier: 156936857

GI number: 156936857

Start: 58650

End: 61010

Strand: Reverse

Name: polB [H]

Synonym: Igni_0062

Alternate gene names: 156936857

Gene position: 61010-58650 (Counterclockwise)

Preceding gene: 156936858

Following gene: 156936854

Centisome position: 4.7

GC content: 49.77

Gene sequence:

>2361_bases
TTGAAGTTCTACGTACTCGACGTGAGCTACGAACTGGAAGGCAACAAACCAGTAATACTAATTTGGGCGCTCGACGAGAA
CGAAAGGAGAGTCCTACTCAAAGACAAGAGGTTTAGACCCTACTTTTATGTGCTCCTAAAAGAGAACGCAGACTTGGAAA
GGCTTAAGAGGAAATTATCCCTCTTGAGTAAGCCCAAGTCTCCCATAATTTCCCTCGAGGTACTTAAGAAAAAGCTCATT
GGTAGGGAAAAGACTGTAATTAAAGTGACTACCGTAATCCCCGAGAGCGTTAGAGAGTACAGAGAAGAGGTCAAGAGGTT
GGAGGAAGTCGCAGACGTACTGGAGGCAGACATAAGGTTTTCGATGAGGTACATAATAGATACCGGCTTAAGGCCTTGTG
GCTGGGCGGAGTTCGAGGTAAAAGAGCTTGGCCCCGAGAAGAGCTACCGAGTAGAAACGGTCTACGAGGTCTTGAACGGA
CCCGAGGCACTGAACATAGATGTCAAACCGAAGTTGAAGACCTTAGCCTTCGACATAGAGGTATATAACAAGTCGGGAAC
CCCCCGACCGGAGAGGGACCCAATAATTTCTATTGCATTGGCCTCCGAAGGCTCCGTAGTCTCTAAGCTCTCCCAAGATA
AGAACGATAAGGACTTGATCGTTTGGTTCAAAAAGGAGATGCTCGAACGAGACCCAGACGTCGTCGTTGGGTACAACAGC
AACAGCTTCGACTGGCCGTACTTGATAGAGAGGAGCAAGGTCGTCGGCGTACGCCTAGACGTGGGACGGAAGGTCGGCGT
AATCCCCACTACCGGCGCCTACGGCCACGTCTCCGTTCCGGGGCGCCTTAACGTGGACGTCTACGACTTTGCTAAAGAGG
TGTACGAAGTTAAGGTGAAGACCTTGGAGAACGTCGCAGATTACTTCAACGTCATGAAAAAGGACGAAAGGCCGTTGATC
CCTCACCACCTAATATACCAGTACTGGGAGGACCCTCAAAAGAGGGACGTCTTGAGGAAGTACAACGAAGCTGACGCCCT
CAGCACGCTGATGCTGGCCGAGCTCTTCATTCCTTTCGGAGAACAGCTGAGCTACCTAACCGGCCTTCCGTTAGACCAAG
TCATGGCGGCGTCCGTCGGCTATAGGGTTGAGTGGTACTTGATGAGAGTTGCCTTCGTCACTAACGAGCTCGTGCCGAAT
AGGGTAGAGAGGAAGGTGGCCAGTTACAAGGGGGCCATAGTGCTGCGCCCGCTAAAAGGGGTACACGAGAACGTCGCCGT
TTTGGACTTCTCGTCGATGTATCCAAACATAATGATAAAGTACAACGTCGGCCCTGACACCTTAGTGAGGCCCGGCGAGA
AGGTGTCGCCCGACGAGGTATACGTCTCGCCGGCCGGTTACATGTTCAAAAAGAGGCCCGACGCGTTCTTCAAAAGGTCA
TTAGTAACCTTGCTAAACTTAAGGAAGGAAATTAAGGAAAGAATGAAGTCGTTAAGCCCCGAAGACCCCTTGTACAAGCT
ATTAGACAATAGGCAAAAGGCAGTGAAGGTGCTCGCCAACGCTCACTACGGCTACATGGGGTGGCCCCACGCCCGGTGGT
ACTGTAGGGAGTGCGCAGAGGCAGTAACGTCGTGGGGGAGAGAGTTGATCTTGAAGGCGATCCAAATGGCCCGGGAACTC
GGACTTAAGGTAATTTACGGGGACACGGACAGCTTGTTCGTAATATATGATAAAGAAAAAGTAGAGAAGTTAATCGAAAG
AATAGAAAAAGAGCTGGGCTTTGAAATAAAGATAGATAAAATATACAAAAAGCTCTTCTTCACGGAAGCGAAAAAGAGGT
ACGCCGGCATCTTAGAGGACGGCCGGATAGACGTAGTAGGGTTCGAGGCCGTCAGAGGCGACTGGGCGGAGGTCGCCAAG
GAGGTCCAAGAAAAAATTATTGAAATAGTTCTTAAAGAAGGTGACGTGAATAAGGCGGTGGAGTACGTCAATTCAATAAT
AGAAAAACTCAGGAGAGGCGAGGTACCTATAGAGAAGTTAGTCATTTGGAAGACGTTAGAGAAATCCTTAGACGAGTATA
AGTCTGAGCCGCCGCACGTAGCGGCTGCGAAGAGGGCCATAGAAAGAGGGTACGAAGTAAAGAAAGGGGACAAGGTAGGC
TACGTGATAGTGAAGGGCGTAGGGAAGCTGTCGAGCAGGGCGTGGCCTTGGTTCTTGGTCCAGAAAGAAATGATCGACTA
CAACTATTACATAGACCATCAAGTCATACCGGCAGCCATGAGGATATTGGGATACTTCGGGATCACTGAGGCTAGGCTCA
AGGGGAGGAAAGACGGCCTTTGGAAGTTCATAAACAAGTAA

Upstream 100 bases:

>100_bases
GAGCGTGCCGATATCCTCGTGGCCGCCCTTCCCGCCCGCCGCCAACTCCAGCACCACCTAAATTTCTCGAAGGCGTTGAA
GGCGAACGGTAGGAAGGACT

Downstream 100 bases:

>100_bases
TAAGGAGAGATTAACTCAAGAGGACTACGAACACGCCCTTAGGCCTCTTGCTGACCCTAGCTACCCTCCTCCCCAGTATC
AATCGGCCTCCCTTCAAGCT

Product: DNA polymerase II

Products: NA

Alternate protein names: DNA polymerase II [H]

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MKFYVLDVSYELEGNKPVILIWALDENERRVLLKDKRFRPYFYVLLKENADLERLKRKLSLLSKPKSPIISLEVLKKKLI
GREKTVIKVTTVIPESVREYREEVKRLEEVADVLEADIRFSMRYIIDTGLRPCGWAEFEVKELGPEKSYRVETVYEVLNG
PEALNIDVKPKLKTLAFDIEVYNKSGTPRPERDPIISIALASEGSVVSKLSQDKNDKDLIVWFKKEMLERDPDVVVGYNS
NSFDWPYLIERSKVVGVRLDVGRKVGVIPTTGAYGHVSVPGRLNVDVYDFAKEVYEVKVKTLENVADYFNVMKKDERPLI
PHHLIYQYWEDPQKRDVLRKYNEADALSTLMLAELFIPFGEQLSYLTGLPLDQVMAASVGYRVEWYLMRVAFVTNELVPN
RVERKVASYKGAIVLRPLKGVHENVAVLDFSSMYPNIMIKYNVGPDTLVRPGEKVSPDEVYVSPAGYMFKKRPDAFFKRS
LVTLLNLRKEIKERMKSLSPEDPLYKLLDNRQKAVKVLANAHYGYMGWPHARWYCRECAEAVTSWGRELILKAIQMAREL
GLKVIYGDTDSLFVIYDKEKVEKLIERIEKELGFEIKIDKIYKKLFFTEAKKRYAGILEDGRIDVVGFEAVRGDWAEVAK
EVQEKIIEIVLKEGDVNKAVEYVNSIIEKLRRGEVPIEKLVIWKTLEKSLDEYKSEPPHVAAAKRAIERGYEVKKGDKVG
YVIVKGVGKLSSRAWPWFLVQKEMIDYNYYIDHQVIPAAMRILGYFGITEARLKGRKDGLWKFINK

Sequences:

>Translated_786_residues
MKFYVLDVSYELEGNKPVILIWALDENERRVLLKDKRFRPYFYVLLKENADLERLKRKLSLLSKPKSPIISLEVLKKKLI
GREKTVIKVTTVIPESVREYREEVKRLEEVADVLEADIRFSMRYIIDTGLRPCGWAEFEVKELGPEKSYRVETVYEVLNG
PEALNIDVKPKLKTLAFDIEVYNKSGTPRPERDPIISIALASEGSVVSKLSQDKNDKDLIVWFKKEMLERDPDVVVGYNS
NSFDWPYLIERSKVVGVRLDVGRKVGVIPTTGAYGHVSVPGRLNVDVYDFAKEVYEVKVKTLENVADYFNVMKKDERPLI
PHHLIYQYWEDPQKRDVLRKYNEADALSTLMLAELFIPFGEQLSYLTGLPLDQVMAASVGYRVEWYLMRVAFVTNELVPN
RVERKVASYKGAIVLRPLKGVHENVAVLDFSSMYPNIMIKYNVGPDTLVRPGEKVSPDEVYVSPAGYMFKKRPDAFFKRS
LVTLLNLRKEIKERMKSLSPEDPLYKLLDNRQKAVKVLANAHYGYMGWPHARWYCRECAEAVTSWGRELILKAIQMAREL
GLKVIYGDTDSLFVIYDKEKVEKLIERIEKELGFEIKIDKIYKKLFFTEAKKRYAGILEDGRIDVVGFEAVRGDWAEVAK
EVQEKIIEIVLKEGDVNKAVEYVNSIIEKLRRGEVPIEKLVIWKTLEKSLDEYKSEPPHVAAAKRAIERGYEVKKGDKVG
YVIVKGVGKLSSRAWPWFLVQKEMIDYNYYIDHQVIPAAMRILGYFGITEARLKGRKDGLWKFINK
>Mature_786_residues
MKFYVLDVSYELEGNKPVILIWALDENERRVLLKDKRFRPYFYVLLKENADLERLKRKLSLLSKPKSPIISLEVLKKKLI
GREKTVIKVTTVIPESVREYREEVKRLEEVADVLEADIRFSMRYIIDTGLRPCGWAEFEVKELGPEKSYRVETVYEVLNG
PEALNIDVKPKLKTLAFDIEVYNKSGTPRPERDPIISIALASEGSVVSKLSQDKNDKDLIVWFKKEMLERDPDVVVGYNS
NSFDWPYLIERSKVVGVRLDVGRKVGVIPTTGAYGHVSVPGRLNVDVYDFAKEVYEVKVKTLENVADYFNVMKKDERPLI
PHHLIYQYWEDPQKRDVLRKYNEADALSTLMLAELFIPFGEQLSYLTGLPLDQVMAASVGYRVEWYLMRVAFVTNELVPN
RVERKVASYKGAIVLRPLKGVHENVAVLDFSSMYPNIMIKYNVGPDTLVRPGEKVSPDEVYVSPAGYMFKKRPDAFFKRS
LVTLLNLRKEIKERMKSLSPEDPLYKLLDNRQKAVKVLANAHYGYMGWPHARWYCRECAEAVTSWGRELILKAIQMAREL
GLKVIYGDTDSLFVIYDKEKVEKLIERIEKELGFEIKIDKIYKKLFFTEAKKRYAGILEDGRIDVVGFEAVRGDWAEVAK
EVQEKIIEIVLKEGDVNKAVEYVNSIIEKLRRGEVPIEKLVIWKTLEKSLDEYKSEPPHVAAAKRAIERGYEVKKGDKVG
YVIVKGVGKLSSRAWPWFLVQKEMIDYNYYIDHQVIPAAMRILGYFGITEARLKGRKDGLWKFINK

Specific function: Thought To Be Involved In DNA Repair And/Or Mutagenesis. [C]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=858, Percent_Identity=27.7389277389277, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI106507301, Length=618, Percent_Identity=28.3171521035599, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI153792012, Length=616, Percent_Identity=25.6493506493506, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1786246, Length=744, Percent_Identity=24.8655913978495, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17559488, Length=741, Percent_Identity=26.9905533063428, Blast_Score=205, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI32565317, Length=610, Percent_Identity=26.0655737704918, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI86563326, Length=630, Percent_Identity=26.1904761904762, Blast_Score=131, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320101, Length=885, Percent_Identity=25.9887005649718, Blast_Score=217, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6325090, Length=615, Percent_Identity=25.5284552845528, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324227, Length=625, Percent_Identity=25.28, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24664937, Length=874, Percent_Identity=26.6590389016018, Blast_Score=201, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24648780, Length=618, Percent_Identity=26.3754045307443, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24586371, Length=622, Percent_Identity=25.08038585209, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90904; Mature: 90904

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYVLDVSYELEGNKPVILIWALDENERRVLLKDKRFRPYFYVLLKENADLERLKRKLS
CEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHH
LLSKPKSPIISLEVLKKKLIGREKTVIKVTTVIPESVREYREEVKRLEEVADVLEADIRF
HHHCCCCCCEEHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMRYIIDTGLRPCGWAEFEVKELGPEKSYRVETVYEVLNGPEALNIDVKPKLKTLAFDIE
HHHHHHHCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
VYNKSGTPRPERDPIISIALASEGSVVSKLSQDKNDKDLIVWFKKEMLERDPDVVVGYNS
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHCCCCCEEEECCC
NSFDWPYLIERSKVVGVRLDVGRKVGVIPTTGAYGHVSVPGRLNVDVYDFAKEVYEVKVK
CCCCCCCEEECCEEEEEEEECCCEECCEECCCCCCEEECCCEECCHHHHHHHHHHHHHHH
TLENVADYFNVMKKDERPLIPHHLIYQYWEDPQKRDVLRKYNEADALSTLMLAELFIPFG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC
EQLSYLTGLPLDQVMAASVGYRVEWYLMRVAFVTNELVPNRVERKVASYKGAIVLRPLKG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEECCCC
VHENVAVLDFSSMYPNIMIKYNVGPDTLVRPGEKVSPDEVYVSPAGYMFKKRPDAFFKRS
CCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
LVTLLNLRKEIKERMKSLSPEDPLYKLLDNRQKAVKVLANAHYGYMGWPHARWYCRECAE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AVTSWGRELILKAIQMARELGLKVIYGDTDSLFVIYDKEKVEKLIERIEKELGFEIKIDK
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCEEEHHH
IYKKLFFTEAKKRYAGILEDGRIDVVGFEAVRGDWAEVAKEVQEKIIEIVLKEGDVNKAV
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
EYVNSIIEKLRRGEVPIEKLVIWKTLEKSLDEYKSEPPHVAAAKRAIERGYEVKKGDKVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEE
YVIVKGVGKLSSRAWPWFLVQKEMIDYNYYIDHQVIPAAMRILGYFGITEARLKGRKDGL
EEEEECCHHHCCCCCCEEEEEHHHHCCEEEECCHHHHHHHHHHHHHCCHHHHHCCCCCCH
WKFINK
HHHHCC
>Mature Secondary Structure
MKFYVLDVSYELEGNKPVILIWALDENERRVLLKDKRFRPYFYVLLKENADLERLKRKLS
CEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHH
LLSKPKSPIISLEVLKKKLIGREKTVIKVTTVIPESVREYREEVKRLEEVADVLEADIRF
HHHCCCCCCEEHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMRYIIDTGLRPCGWAEFEVKELGPEKSYRVETVYEVLNGPEALNIDVKPKLKTLAFDIE
HHHHHHHCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEE
VYNKSGTPRPERDPIISIALASEGSVVSKLSQDKNDKDLIVWFKKEMLERDPDVVVGYNS
EECCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHCCCCCEEEECCC
NSFDWPYLIERSKVVGVRLDVGRKVGVIPTTGAYGHVSVPGRLNVDVYDFAKEVYEVKVK
CCCCCCCEEECCEEEEEEEECCCEECCEECCCCCCEEECCCEECCHHHHHHHHHHHHHHH
TLENVADYFNVMKKDERPLIPHHLIYQYWEDPQKRDVLRKYNEADALSTLMLAELFIPFG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC
EQLSYLTGLPLDQVMAASVGYRVEWYLMRVAFVTNELVPNRVERKVASYKGAIVLRPLKG
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEECCCC
VHENVAVLDFSSMYPNIMIKYNVGPDTLVRPGEKVSPDEVYVSPAGYMFKKRPDAFFKRS
CCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
LVTLLNLRKEIKERMKSLSPEDPLYKLLDNRQKAVKVLANAHYGYMGWPHARWYCRECAE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
AVTSWGRELILKAIQMARELGLKVIYGDTDSLFVIYDKEKVEKLIERIEKELGFEIKIDK
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCCEEEHHH
IYKKLFFTEAKKRYAGILEDGRIDVVGFEAVRGDWAEVAKEVQEKIIEIVLKEGDVNKAV
HHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
EYVNSIIEKLRRGEVPIEKLVIWKTLEKSLDEYKSEPPHVAAAKRAIERGYEVKKGDKVG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEE
YVIVKGVGKLSSRAWPWFLVQKEMIDYNYYIDHQVIPAAMRILGYFGITEARLKGRKDGL
EEEEECCHHHCCCCCCEEEEEHHHHCCEEEECCHHHHHHHHHHHHHCCHHHHHCCCCCCH
WKFINK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]