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The map label for this gene is kefA [C]

Identifier: 156936851

GI number: 156936851

Start: 53197

End: 53970

Strand: Reverse

Name: kefA [C]

Synonym: Igni_0056

Alternate gene names: 156936851

Gene position: 53970-53197 (Counterclockwise)

Preceding gene: 156936854

Following gene: 156936849

Centisome position: 4.16

GC content: 44.19

Gene sequence:

>774_bases
TTGACGCCCGTGGACGTTCTGAGTATTGTAGTGTTGCTGTCGATATATCTAATCATCAGAAAAGTTGGAGAGGACAAGCT
GAGTGAACTCTTGGTCAGAAGCTTAGGCGACGAAAACCTAGCGTTTATGGGCGTTCGCATAATAACGTACCTCATTTACG
CACTTTTGATAATGGCTTTCTTGTCGGCAATAGGAGTCCCAATACAAAACTTGCTGATGGCAGGTGGTATATTAACCTTG
GCTATTTCGTTCGCAGCTCAAACAGTAATATCAAACGCGATATCCGGCCTATTCCTAATGATTGAAAGACCCCTAAAAAT
AGGGGACTTTGTCGAGCTCAAGAATAGCGGCATCAGCGGGAAAGTCCAATCTTTCAACATATTTTCTACTATGCTACTCA
CCATAGACGGCGAGTTAGCGCGCATACCTAACTCAACCTTCTTCAATGACATTATAATCAATAAGACTGGAACTGTTGCA
AGACGCCTCCAAGTACGGGTCGGCGTAAGCTATTCTGACGCGTTGAAGGCGTTAGACGTCTTTAGGAGTTACTTCTCCAA
ACACCCCGACGTCTTGCAAGTACCCGAGCCAGAGATATTCATCGAAGATTTCGGCGACTTCTACGTCCTAATAAGGCTTA
ACTTGTGGGTGCCCACGAAGAAGTGGTATGATATGTACAAATCAATCCGTGAGGAAATATTCAAGGTGTGCCAGAAGTAC
AACATTGAGATACCGTTCCCTATACACGTAGTCTACCTCAAAGACCAAAAATAG

Upstream 100 bases:

>100_bases
GCCTCGGTGCACGGCCTCGTCTCCTCACGAGTTCGGCTGAAACACGGGTCCTCATCGGCTTTTACGACCCTGGCACACGC
GTCTCGTCGGAAGTAAAGCG

Downstream 100 bases:

>100_bases
CCACCCACCTAGGAACCTTCCCCGTGTCCGGGACTTCCACGTAACCTTGCTTTCTGGGTGTGTCCCTCAGCCTTCTGATC
GTAGCCACGCCCCTCTCTAT

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTPVDVLSIVVLLSIYLIIRKVGEDKLSELLVRSLGDENLAFMGVRIITYLIYALLIMAFLSAIGVPIQNLLMAGGILTL
AISFAAQTVISNAISGLFLMIERPLKIGDFVELKNSGISGKVQSFNIFSTMLLTIDGELARIPNSTFFNDIIINKTGTVA
RRLQVRVGVSYSDALKALDVFRSYFSKHPDVLQVPEPEIFIEDFGDFYVLIRLNLWVPTKKWYDMYKSIREEIFKVCQKY
NIEIPFPIHVVYLKDQK

Sequences:

>Translated_257_residues
MTPVDVLSIVVLLSIYLIIRKVGEDKLSELLVRSLGDENLAFMGVRIITYLIYALLIMAFLSAIGVPIQNLLMAGGILTL
AISFAAQTVISNAISGLFLMIERPLKIGDFVELKNSGISGKVQSFNIFSTMLLTIDGELARIPNSTFFNDIIINKTGTVA
RRLQVRVGVSYSDALKALDVFRSYFSKHPDVLQVPEPEIFIEDFGDFYVLIRLNLWVPTKKWYDMYKSIREEIFKVCQKY
NIEIPFPIHVVYLKDQK
>Mature_256_residues
TPVDVLSIVVLLSIYLIIRKVGEDKLSELLVRSLGDENLAFMGVRIITYLIYALLIMAFLSAIGVPIQNLLMAGGILTLA
ISFAAQTVISNAISGLFLMIERPLKIGDFVELKNSGISGKVQSFNIFSTMLLTIDGELARIPNSTFFNDIIINKTGTVAR
RLQVRVGVSYSDALKALDVFRSYFSKHPDVLQVPEPEIFIEDFGDFYVLIRLNLWVPTKKWYDMYKSIREEIFKVCQKYN
IEIPFPIHVVYLKDQK

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=216, Percent_Identity=31.0185185185185, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787591, Length=261, Percent_Identity=22.9885057471264, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789291, Length=256, Percent_Identity=25.390625, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI2367355, Length=254, Percent_Identity=21.259842519685, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 29079; Mature: 28948

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVDVLSIVVLLSIYLIIRKVGEDKLSELLVRSLGDENLAFMGVRIITYLIYALLIMAF
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LSAIGVPIQNLLMAGGILTLAISFAAQTVISNAISGLFLMIERPLKIGDFVELKNSGISG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
KVQSFNIFSTMLLTIDGELARIPNSTFFNDIIINKTGTVARRLQVRVGVSYSDALKALDV
CEEHHHHHHHHHHHCCCHHHHCCCCCHHHHEEECCCCHHHHHHHHEECCCHHHHHHHHHH
FRSYFSKHPDVLQVPEPEIFIEDFGDFYVLIRLNLWVPTKKWYDMYKSIREEIFKVCQKY
HHHHHHCCCCEEECCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
NIEIPFPIHVVYLKDQK
CCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TPVDVLSIVVLLSIYLIIRKVGEDKLSELLVRSLGDENLAFMGVRIITYLIYALLIMAF
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LSAIGVPIQNLLMAGGILTLAISFAAQTVISNAISGLFLMIERPLKIGDFVELKNSGISG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
KVQSFNIFSTMLLTIDGELARIPNSTFFNDIIINKTGTVARRLQVRVGVSYSDALKALDV
CEEHHHHHHHHHHHCCCHHHHCCCCCHHHHEEECCCCHHHHHHHHEECCCHHHHHHHHHH
FRSYFSKHPDVLQVPEPEIFIEDFGDFYVLIRLNLWVPTKKWYDMYKSIREEIFKVCQKY
HHHHHHCCCCEEECCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
NIEIPFPIHVVYLKDQK
CCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]